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  • Küçük Resim Yok
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    Flor salınımı yapan ortodontik kompozitlerin antibakteriyel etkileri: Pilot çalışma
    (2011) Öztürk, Fırat; Toy, Ebubekir; Arslan, Uğur; Hatunoğlu, Erdem
    Amaç: Bu çalışmanın amacı Direkt Kontakt Testi (DKT) kullanılarak; üç farklı tipte flor salınımı yapan ortodontik kompozitin antibakteriyel özelliklerinin değerlendirilmesidir. Gereçler ve Yöntem: Flor salınımı yapan ortodontik kompozitlerin antibakteriyel özellikleri DKT kullanılarak değerlendirildi. DKT için doksan altı kuyucuklu kültür kabının duvarları her grupta 8 örnek olacak şekilde test materyalleri ile [Transbond Plus (3M Lnitek, Monrovia, ABD) Light Bond (Reliance Ortho Prod. Inc. Itasca, İL, ABDi Kurasper F 'Kuraray Medical Inc., Okayama, laponya)] kaplandı. Pozitif kontrol materyali olarak Kalzinol (çinko oksit öjenol siman) Dentsply, Konstanz, Almanya) kullanıldı. Streptococus mutans süspansiyonu örneklerin yüzey alanına uygulanarak î7C'de bir saat bekletildi. Bakteri üremesi, ısı kontrollü spektrofotometre ile 16 saat boyunca gözlemlendi. Herbir kuyucuktaki üremenin kinetiği her 30 dakikada bir 650 nm düzeyinde sürekli olarak kaydedildi. Veriler tek yönlü ANOVA testinin yanı sıra TamHane'ın T2 çoklu karşılaştırma testi ile incelendi. İstatistiksel önem seviyesi P0.05 olarak belirlendi. Bulgular: DKT sonuçlarının değerlendirilmesi dört grup arasında istatistiksel olarak anlamlı farklar olduğunu ortaya koymuştur. (F 156,951, P 0.000). Kontrol materyali olan Kalzinol bakteri üremesini tamamen engellemiştir (ortalama 0.01 0.05). Çoklu karşılaştırma testi, Kalzinol ve Transbond Plus {ortalama 0.120.17) arasında anlamlı bir fark olmadığını göstermiştir (P 0.509) Transbond Plus, Kalzinol ile hemen hemen benzer etkinlik sergilemiştir (Şekil I). Kurasjjer F (ortalama 0.730.06) ve Light Borxl (ortalama 0.850.02) ise KalzinoVle karşılaştırıldığında istatistiksel olarak anlamlı bir fark göstermiştir (P 0.000) Sonuç: Transbond Plus belirgin antibakteriyel etkinlik göstermiştir. (Türk Ortodonti Dergisi 2011;24:3-12)
  • Küçük Resim
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    Long-term antibacterial properties of fluoride-releasing orthodontic bonding adhesives
    (2012) Öztürk, Fırat; Toy, Ebubekir; Arslan, Uğur; Hatunoğlu, Erdem; Aksakallı, Sertaç
    Objectives: The aim of this study was to compare antibacterial properties of 3 different fluoride-releasing orthodontic adhesives during three months by using the direct contact test (DCT). Materials and Methods: The materials tested in the present study were Transbond Plus (3M Unitek, Monrovia, USA), Light Bond (Reliance Ortho Prod. Inc. Itasca, IL, USA), and Kurasper F (Kuraray Medical Inc., Okayama, Japan). Eight specimens of each material type were prepared for estimation. For the DCT, wells of 96-microtitre plates were coated with each of three bonding adhesives. Eight uncoated wells, using identical inocula size, served as a positive control (bacteria with medium). A Streptococcus mutans suspension was placed on the surface of each specimen that was held for 1 hour at 371/2C. Bacterial growth was monitored for 16 hours with a temperature-controlled microplate spectrophotometer. The kinetics of the growth in each well was recorded continuously at 650 nm in every 30 minutes. Three additional measurements were performed on these tested materials after aging for 1 week, 1 month and 3 months. One-way ANOVA and Tamhane's T2 multiple comparison tests were applied to the data. The level of significance was set as p<0.05. Results: The DCT showed that there were significant differences found in freshly mixed samples between four groups (p<0.001, F=20.901). Freshly mixed samples of Transbond Plus showed antibacterial activity (p<0.001). Kurasper F and Light Bond did not differ from positive control. In the measurements of aged specimens, all materials showed an increase in the logarithmic growth rate of Streptococcus mutans. Conclusion: Only Transbond Plus showed antibacterial properties in freshly mixed specimens. However, none of the tested orthodontics composites showed antibacterial properties after aging.
  • Küçük Resim Yok
    Öğe
    Real-time cell analysis of the cytotoxicity of the components of orthodontic acrylic materials on gingival fibroblasts
    (MOSBY-ELSEVIER, 2011) Öztürk, Fırat; Malkoç, Sıddık; Ersöz, Mustafa; Hakkı, Sema S.; Bozkurt, Buket S.
    Introduction: The aim of this study was to evaluate the cytotoxicity of 3 orthodontic acrylic materials and 2 manipulation methods. Methods: The orthodontic acrylic materials Orthocryl EQ (Dentaurum, Ispringen, Germany), Orthoplast (Vertex Dental, Zeist, The Netherlands), and O-80 (Imicryl, Konya, Turkey) were prepared with 2 polymerization methods (doughing and spray on). Totally, 60 cylinders (5 x 2 mm), fabricated by using a different acrylic and method, were divided into 6 groups. Gingival fibroblasts were isolated from gingival connective tissue of systemically healthy subjects. Materials were incubated in Dulbecco's modified eagle's medium culture medium (Biological Industries, Beit Haemek, Israel) for 72 hours according to ISO 10993-5 standards (surface area to volume ratio of the specimen to cell-culture medium: 3 cm(2)/mL). Gingival fibroblasts were maintained with Dulbecco's modified eagle medium containing 10% fetal bovine serum. A real-time cell analyzer (RT-CES, xCELLigence; Roche Applied Science, Mannheim, Germany, and ACEA Biosciences, San Diego, Calif) was used to evaluate cell survival. After seeding 200 mu L of the cell suspensions into the wells (20,000 cells/well) of the E-plate 96, gingival fibroblasts were treated with bioactive components released by the acrylic materials (1/1 and 1/2 dilutions) and monitored every 15 minutes for 121 hours. For the proliferation experiments, the statistical analyses used were 1-way analysis of variance (ANOVA) and Tukey-Kramer multiple comparisons tests. Results: There was no significant difference between the cell indexes of the control and study groups for the 1/1 and 1/2 dilutions at 21 and 32 hours. When evaluated at 68 hours, all 1/2 dilutions of acrylic materials showed statistically insignificant differences (P >0.05) except for Orthoplast (P <0.05). But all acrylic materials were different from the control group in the 1/1 dilutions (P <0.001). At 121 hours, all test groups were significantly different from the untreated control group (P <0.001). Conclusions: The results indicate that the long cycle increased the cytotoxicity of the tested materials, and there was no significant difference between the spray-on and doughing methods on cytotoxicity. (Am J Orthod Dentofacial Orthop 2011; 140:e243-e249)