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    The Effects of Laser with Different Parameters and Crosshatching on Fibroblast Adhesion and Proliferation to Implant Surfaces
    (QUINTESSENCE PUBLISHING CO INC, 2017) Celebi, Hakki; Arpaci, Pembegul Uyar; Celik, Ilhami; Akman, Serhan
    Purpose: To compare the adhesion and proliferation effect of HGF-1 cells on pure titanium disks when the surfaces are unprocessed (machined surfaces after slicing) or modified with a laser. Materials and Methods: Twenty-eight titanium disks were divided into four groups. Three surface topographies were created using an erbium fiber laser: group 1 (unidirectional application [no crosshatch]), group 2 (crosshatching in two directions), and group 3 (crosshatching in three directions). The samples in group 4 were unprocessed and served as controls. The surface roughness of samples was investigated with scanning electron microscopy (SEM) and measured with a mechanical surface profilometer. HGF-1 cell line was used to analyze the adhesion of fibroblasts on the sample surfaces. The authors used SEM, XTT, and acridine orange tests to determine the adhesion of HGF-1 onto specimen surfaces and the cell morphology after incubation for 72 hours. XTT results and surface roughness values for all specimens were analyzed using the Kruskal-Wallis test. Results: Ra values of group 1 to group 4 (control) were 1.13, 0.26, 0.38, and 0.19, respectively. Crosshatching decreased the surface roughness values compared with unidirectional application (P = .003). Cell morphology observed in SEM showed that the elongation direction of cells resulted in all directions related with additional filopodia extentions within the crosshatch groups. Therefore, the highest cell viability was also detected in the three-directional crosshatch group (group 3) (P = .000) in XTT assay. According to the acridine orange test, higher cell numbers were seen in group 3, similar to the XTT findings (P = .86). Conclusion: Crosshatching significantly increased the cell-covered implant surfaces compared with the unidirectional group. Decreasing the surface Ra values via crosshatching helped spread the fibroblast over the implants in any direction, thus increasing cell proliferation and adhesion.
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    Effects of Novel Root Repair Materials on Attachment and Morphological Behaviour of Periodontal Ligament Fibroblasts: Scanning Electron Microscopy Observation
    (WILEY-BLACKWELL, 2016) Akbulut, Makbule Bilge; Arpaci, Pembegul Uyar; Eldeniz, Ayce Unverdi
    The aim of this study was to evaluate the adhesion of periodontal ligament fibroblasts (PDLs) on newly proposed root repair materials [Biodentine, MM-MTA, polymethylmethacrylate (PMMA) bone cement, and SDR], in comparison with contemporary root repair materials [IRM, Dyract compomer, ProRoot MTA (PMTA), and Vitrebond]. Five discs from each material were fabricated in sterile Teflon molds, and the specimens were aged and prewetted in cell culture media for 96 hours. Three material discs were used for scanning electron microscopy (SEM) for the assessment of the attachment, density, and morphological changes in the PDLs, while two samples were used for energy dispersive x-ray spectroscopy (SEM-EDX) to determine the elemental composition of the materials. Human PDLs were plated onto the materials at a density of 10,000/well, and incubated for 3 days. The SEM micrographs were taken at different magnifications (5003 and 50003). In the SEM, the cells were attached and well spread-out on the surfaces of the Biodentine, PMTA, and Dyract compomer, while varied cell densities and morphological alterations were observed in the Vitrebond, IRM, MM-MTA, SDR, and PMMA bone cement groups. The SEM-EDX analysis revealed a maximum calcium percentage in the PMTA specimens, as well a maximum silicon percentage in the Dyract compomer specimens. This in vitro study demonstrated that the Biodentine and Dyract compomer supported PDL cell adhesion and spreading. The PMTA presented a favorable scaffold for better attachment of the PDL cell aggregates. Therefore, the calcium and silicon content of a material may enhance the PDL cell attachment.