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Öğe Brucella suşlarının identifikasyonu ve biyotiplendirilmesi(2009) Aras, Zeki; Ateş, Mehmet; Uçan, Uçkun SaitDünyanın önemli zoonoz hastalıklarından olan Brusellozis; hayvanlarda genital organlara yerleşerek yavru atmalara ve infertiliteye neden olmakta, endemik olduğu ülkelerde ekonomiyi olumsuz yönde etkilemektedir. Bu derlemede hayvanlarda brusellozisin teşhisi ve etkenlerin tiplendirilmesi ile ilgili moleküler metotlar aktarıldı. Serolojik metodlar derlemenin kapsamı dışındadır.Öğe Hayvansal Dokularda Anti?bi?yoti?k Kalıntılarının Agar Di?ffüzyon Tekni?ği? ile Tayi?ni?(Selçuk Üniversitesi, 1987 Ocak) Acet, Ahmet; Ateş, Mehmet; Erganiş, OsmanAfter oxytetracyline (20 mg/kg) and tetracycline (50 mg/ kg) were given orally to the broiler chickens, antibiotic residues in tissues were determined by agar diffusion technique. Bacillus cereus (ATCC 11778) was used as a sensitive strain. Petri dishes punched at 8 mm in diameter were incubated at 37°C overnight. A total of 124 tissues was tested. The oxytetracycline and tetracycline residues that have been found in tissues were respectively 0.32-2.56 µg/gr and 0.080-0.240 g/gr. The limites of the test strain sensitivity were determined 0.04 pg and 0.01 g levels for oxytetracycline and tetracycline respectively.Öğe İneklerde Mastitislerin Patolojik ve Bakteriyolojik İncelenmesi(Selçuk Üniversitesi, 1996) Erer, Hüdaverdi; Ateş, Mehmet; Kıran, Metin Münir; Çiftçi, M. Kemal; Kaya, OsmanDiese Arbeit wurde bei im Schlachthof (Konya) zum Schlachten vorgestellten Kühen durcgeführt. Das Ziel der Arbeit war die Haufigkeit der Mastitis und die pathologisch-anatomische sowie hi- stopathologische Veränderungen an der Euterentzündungen. Von 883 zur Untersuchung vorgestellten Kühen wurde bei 118 Kühen (13.36 %) mittels CMT und klinische Untersuchungen eine Euterentzündung festgestellt. Von diesen erkranten Kühen wurden 232 Euterviertel zur Untersuchung vorgestellt. Aus den CMT positiven Eutervierteln wurden Miichproben gesammelt. Diese Milchproben wurden im aeroben, anaeroben und mikroaerophilen Bedingungen gezüchtet und danach bakteriologisch und mykologisch untersucht. Von den CMT positiven 125 Eutervierteln wurden pathogene Mikroorganismen isoliert und in Reihenfolge; Staph aureus (47.3 %), C. pyogenes (16.3%), E. coli (8.2 %), C. albicans (6.5%) Str. agalactiae (6%). Staph. epidermidis (4.3 %), Kl. pneumoniae (3.8%), B. subtilis (2.2%). Str. dysgalactiae (2.2%) Flavobact, spp. (1.6%), B. cereus (1.1. %) und Pr. mirabilis (0.5%) identifiziert. Nach den histopathologischen Untersuchungen wurden folgende Mastitisformen festgestellt: Akute katarrhalisce Mastitis und Galaktophoritis (10), Chronische katarrhalische Mastitis und Galaktophoritis (142), Abszessbildende Mastitis (39), Interstitielle nichteitrige Mastitis (21). Chronische Mastitis (12), Haemorrhagisch-nekrotisierende Mastitis (5). Lobularinfiltrierende Eutertuberkulose (3).Öğe Isolation of Ornithobacterium Rhinotracheale From Chickens and Turkeys(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY, 2002) Erganiş, Osman; Ateş, Mehmet; Hadimli, H. H.; Çorlu, MehmetA total of 251 tracheal swabs and a total of 102 tracheae, lungs, airsacs, livers and spleens were collected from commercial layer, broiler and broiler parent stock flocks with respiratory infection. From turkey flocks, 96 tracheal swabs and 5 tissue samples from the trachea, lung, airsac, liver and spleen were also taken. Cultivations on Blood Agar supplemented with 51 10%sheep blood, on Chocolate Agar and on MacConkey Agar were incubated at 37degreesC for 24-72 hours in the presence of 10%CO(2)Gram staining was carried out for the smooth and non-hemolytic colonies 1-3 mm in diameter and for those showing grayish pigmentation after approximately 48 hours of culture. Cram negative pleomorphic rods were considered to be Ornithobacterium rhinotracheale (ORT) and other conventional tests were made as follows: hemolysis, motility, spore formation, growth on MacConkey and TSI agars, catalase, indol, sorbitol, inositol, rafinose, trehalose, xylose, dulcitol, nitrate, methyl red, oxidase, beta-D galactosidase, urease, glucose, galactose, lactose, maltose, fructose and Voges-Proskauer. ORT was isolated from none of the samples taken from turkeys, broiler or broiler parent stock flocks. However, two ORT isolations were achieved from two commercial pullets aged 12 and 15 weeks. One of these isolates was serotyped as "B" by the ACP test while the other was not found to belong to any of the serogroups. Both of the isolates were found to be susceptible to oflaxacin, erythromicin, lincomycin, amoxicillin, and amoxicillin+clavulonic acid by antibiotic suspectibility tests.Öğe Isolation of Ornithobacterium Rhinotracheale From Chickens and Turkeys(2002) Erganiş, Osman; Ateş, Mehmet; Hadimli, Hasan Hüseyin; Çorlu, MehmetAbstract: A total of 251 tracheal swabs and a total of 102 tracheae, lungs, airsacs, livers and spleens were collected from commercial layer, broiler and broller parent stock flocks with respiratory infection. From turkey flocks, 96 tracheal swabs and 5 tissue samples from the trachea, lung, airsac, liver and spleen were also taken. Cultivations on Blood Agar supplemented with 5- 10%sheep blood, on Chocolate Agar and on MacConkey Agar were incubated at 37°C for 24-72 hours in the presence of 10%CO2- Gram staining was carried out for the smooth and non-hemolytic colonies 1-3 mm in diameter and for those showing grayish pigmentation after approximately 48 hours of culture. Gram negative pleomorphic rods were considered to be Ornithobacterium rhinotracheale (ORT) and other conventional tests were made as follows: hemolysis, motility, spore formation, growth on MacConkey and TSI agars, catalase, indol, sorbitol, inositol, rafinose, trehalose, xylose, dulcitol, nitrate, methyl red, oxidase, B-D galactosidase, urease, glucose, galactose, lactose, maltose, fructose and Voges-Proskauer. ORT was isolated from none of the samples taken from turkeys, broiler or broiler parent stock flocks. However, two ORT isolations were achieved from two commercial pullets aged 12 and 15 weeks. One of these isolates was serotyped as "B" by the AGP test while the other was not found to belong to any of the serogroups. Both of the isolates were found to be susceptible to oflaxacin, erythromicin, lincomycin, amoxicillin, and amoxicillin+clavulonic acid by antibiotic suspectibility tests.Öğe Konya Bölgesi?ndeki? I?shalli? Buzağılardan I?zole Edi?len E. Coli?'leri?n, Bi?yoki?myasal, Anti?bi?yoti?klere Duyarlılık ve Bulaşıcı Tip Plazmi?d (R-Faktör) Taşıma Özelli?kleri?ni?n I?ncelenmesi?(Selçuk Üniversitesi, 1987 Ocak) Ateş, Mehmet; Sezen, İ. Yavuz; Erganiş, Osman; Çorlu, MehmetIn this study, some biochemical characteristics, different antibiotics resistance states and R-factor transmissibility of 51 E. coli strains isolated from calves with diarrhea up to one week age were examined. Biochemical characteristics of the strains were found to be as follows: The rates for glucose, lactose, mannitol dulsitol, arabinose and adonitol fermentation tests were 100 %, 100%, 100%, 54.9 %, 98.1 % and 21.6 % respectively and the rates for urease production, citrate utilization, tryptophane deaminase, H&S, indol and motility tests were 100 %, 0.0 %, 0.0%, 0.0 %, 100%, 0.0 %, 96.1 % and 80.4% respectively. It was observed that only one of the whole strains (1.96 %) possessed a hemolytic activity. According to the results of disk diffusion method, antibiotic resistance rates of E. coli strains were determined as follows 70.5 % for tetracyline, 86.2% for oxytetracycline and neomisin, 98.1 % for erytromisin, 54.9% for ampisilin, 49.0% for streptomisin, 23.4 % for chloramphenicole and 5.9% for nalidixic acide. Of the strains 94.1 per cent were resistant to more than 3 antibiotics. The rates of R plasmid transmission of the strain were found to be 27.1 per cent.Öğe Konya bölgesinde tavuklarda Marek Hastalığı, Lenfoid Löykoz ve Retiküloendoteliozis' in ELISA ve histopatolojik yöntemler ile araştırılması(2003) Uçan, Uçkun Sait; Ateş, Mehmet; Kıran, M. Metin; Hadımlı, H. Hüseyin; Ortatatlı, Mustafa; Kuyucuoğlu, E. ÖzlemKanatlı hayvanların etiyolojisi bilinen neoplastik hastalıklar grubu içerisinde yer alan 3 tane viral kökenli neoplazması vardır. Bunlar, Marek Hastalığı (MH), Lenfoid Löykoz (LL) ve Retiküloendoteliozis (RE)'dir. Bunlardan MH'da kötü huylu T hücre transformasyonu, LL'da ise iyi huylu ve kötü huylu tümör oluşumu görülür. RE lenfomaların oluşumu ile karakterize bir enfeksiyodur. Çalışmanın hayvan materyali, anket çalışması ile belirlendi. Konya ve Çevresindeki ticari ve damızlık sürülerden 540 adet kan ve serum örneği ile 15 doku (karaciğer, böbrek, dalak, bursa Fabricius, pankreas, akciğerler, bezsel ve kaslı mide, Nervus ischiadicus, deri, beyin ve beyincik) örneği toplandı. Bu çalışmada histopatoloji, ve serolojik (ELISA) tanı yöntemleri kullanılarak, seçilen işletmelerden elde edilen örneklere ait kan ve dokulardan her iki yöntem ile kesin tanı konan enfeksiyonlara ait bazı epidemiyolojik veriler derlendi. Çalışmada, tanı amacıyla kullanılabilecek histopatolojik metod ile ELISA arasında istatistiksel bir ilişki olduğu görüldü (p0,05). ELİSA ile, 56 serumda anti-MHV antikoruna rastlanırken, 1 serum örneğinde anti-LLV antikoru tespit edildi. Çalışmadan elde edilen epidemiyolojik bulgular; MH ve LL'un prevalansları sırası ile % 10 ve % 0.2 olarak tespit edildi, incelenen örneklerde RE varlığına dair bir bulguya rastlanmadı.Öğe Konya I?l Mekezi?ni?n Deği?şi?k Semtleri?nden Toplanan Toz Numuneleri?ni?n Bakteri?yoloji?k Yönden Araştırılması(Selçuk Üniversitesi, 1986 Ocak) Sezen, İ. Yavuz; Çorlu, Mehmet; Erganiş, Osman; Ateş, MehmetEs wurden 52 Bodenproben Umgebung von Konya über bakteriologische Erreger untersucht. Aus 52 Proben wurden 138- Erreger isoliert, davon 117 (%85) konnten identifiziert werden. Die identifizierten Bakterien sind Mycobacterium sp., Enterobacter sp., Salmonella sp., E. coli, Serratia, Bacillus cereus, Staphylococcus sp.Öğe Konya ilinde atık yapmış koyun sürülerinde brusellozisin seroprevalansı ve risk faktörlerinin değerlendirilmesi(2009) Aras, Zeki; Ateş, MehmetBrusellozis; hayvanlarda özellikle genital organlara yerleşerek yavru atmalara ve infertiliteye neden olan zoonoz bir hastalıktır. Konya bölgesindeki atık yapmış ve Brucella melitensis (B. melitensis) izole edilmiş koyun sürülerinde, Brusellozis’in yaygınlığını serolojik olarak belirlemek amacıyla 24 ayrı sürüden alınan 588 adet kan serumu RBPT, Mikro Serum Aglütinasyon Testi (mSAT) ve ELISA testleri ile yoklandı. Ayrıca, sürülere ait risk faktörlerinin değerlendirilmesi amacıyla, sürü sağlığı ve yönetimi ile ilgili bilgileri amaçlayan anket hazırlandı. Kan serumlarının 242’si (% 41.1) RBPT ile, 219’u (% 37.2) mSAT ile ve 254’ü (% 43.2) ELISA testi ile pozitif olarak belirlendi. Anket ile sürülere ait epidemiyolojik veriler ortaya konuldu. Konya’da B. melitensis’e bağlı atık görülen koyun sürülerinde % 24-81.8 gibi yüksek oranlarda enfekte hayvan bulunduğu ve bu yüksek oranın halk sağlığı ve ülke hayvancılığı açısından tehdit oluşturduğu kanısına varıldı.Öğe Koyun Masti?ti?sleri? Üzeri?nde Mi?krobi?yolojik İncelemeler(Selçuk Üniversitesi, 1990 Ocak) Ateş, Mehmet; Erganiş, Osman; Kaya, Osman; Çorlu, MehmetThis investigation was performed on 800 milch sheep belonged two state farm in Konya region. Sheep were examined by clinical procedures and tested with California Mastitis test (CMT). Milk samples taken from sheep with mastitis (CMT scores, +++++) were cultured in aerobic, microaerophlic and anaerobic conditions for bacteriological and mycological examination bacteria isolated and identified were tested against Ampicillin, Streptomycin, Tetracyclin, Penicillin, Kanamycin, Erytromycin, Gentamycin and Chloramphenicol discs (Difco). In this area, in the mammary glands of 13.5 % of the tested sheep, mastitis was detected. Although the rates of clini cal mastitis was 0.75 percent, subclinical mastitis in sheep was 12.75 percent. Microbiologic isolation were succeded from (75.0 %) 81 samples of 108 mastitic milk. But, there was mix infection in 8 samples. The percent values of isolated microorganisms were found as below 57.3 % Staph. aureus, 9.0 % for E. coli, 7.9 % for Bacillus ssp. 5.0 % for B. cereus, 5.0 % for C. pyogenes (Actinomyces pyogenes), 3.4 % for Str. agalactiae, 3.4 % for C. pyogenes (Actinomyces pyogenes), 3.4 % for Str. agalacti- ac, 3.4 % for K. pneumoniae, 3.4 % for Candida albicans, 2.2 % for B. subtilis, 1.1 % for Str. dysgalactiae and 1.1 for Ente- robacter ssp. In antibiotic susceptibility tests, Staph. aureus isolates were found to be sensitive to Tetracyclin, Chloramphenicol and Erytromycin, at the rates of 76.0 %, 58.0 % and 38.0 %, respectively, E. coli strains were sensitive to Streptomycin (100%) and Tetracyclin (100 %), C. pyogenes strains were sensitive at the rates of 100 %, 80.0 % and 80.0 %, for Penicil- lin, Streptomycin and Chloramphenicol, respectively.Öğe Masti?ti?sli? I?nek Sütleri?nden I?zole Edi?len Staphylococcus Aureus'larda, Peni?si?li?n Rezi?stansi ile Bazı Enzi?m Karakterleri? ve Bi?yoki?myasal Testler Üzeri?nde Çalışmalar(Selçuk Üniversitesi, 1986 Ocak) Sezen, İ. Yavuz; Erganiş, Osman; Çorlu, Mehmet; Ateş, MehmetNineteen strains of Staph. aureus isolated from cows milk with mastitis were tested for their ability to produce coagulase, phosphatase, catalase, B-lactamase, clumping factor, oxydation/fermentation (O/F) and resistance of penicillin and ampicillin. All of the strains have produced B-lactamase and their resistance rates to penicillin and ampicillin were 94.9%, 47.9% respectively. Catalase, phosphatase and O/F producing rate of all Staph. aureus were positive 100%. The Coagulase tests found to be positive at the rate of 89.8% with rabbit plasma, 73.7% with cow plasma, 100% with mixture of rabbit-cow plasma. The rate of positive clumping factors was also found to be 89.8%, 94.9%, 100% with rabbit, cow and mix plasma, respectively. 73.7% of all strains have grown on Mannitol Salt Agar plates. 12 strains produced - golden cooured colonies. All of the 6 strains were intracutaneously inoculated to rabbits reduced necrosis.Öğe Masti?ti?sli? Süt I?nekleri?nden I?zole Edi?len Stafilokokların ß-Laktamaz Akti?vi?teleri? ve Anti?bi?yoti?klere Duyarlılıkları(Selçuk Üniversitesi, 2001) Ateş, Mehmet; Güler, Leyla; Öncel, Taner; Hadimli, H. Hüseyin; Kav, KürşatBu çalışmada, mastitisli süt ineklerinden izole edilen 82 koagulaz pozitif (78 Staphylococcus aureus, 4 S. intermedius) ve 25 koagulaz negatif (15 S. epidermidis, 4 S. simulans, 2 S. chromogenes, 2 S. saprophyticus, 2 S. caprae) toplam 107 stafilokok suşunun 3-laktamaz aktiviteleri ve antibiyotiklere duyarlılıklan belirlendi. Koagulaz pozitif sta filokoklardan 36'sı (% 46.1) (S. aureus) ve koagulaz negatif suştanın 12'si (% 48) (10 S. epidermidis. 2 S. simulans) - laktamaz pozitif bulundu. Antibiyotik duyarlılık testlerine göre; 107 stafilokok suşunun 105'i (% 98.1) enrofloksastin'e, danofloksasilin'e, amoksisilin+ klavulonik asit'e, 104'ü (% 97.1) sefaperazon+sulbaktam'a, 981 (% 91.5) kloksasilin'e. 93'u (%) 86.9) selaperazon'a, 86'sı (% 80.3) oksasilin'e, 85i (% 79.4) oksitetrasiklin'e, 83'ü (% 77.5) ampisilin+sulbaktam'a, 801 (% 74.7) gentamisin'e ve 76's (% 71.0) trimetoprim+ sulfametaksazola duyarlı bulundu. Ayrıca, stafilokok suşlarının 67'si (% 62 3) ampisilin'e, 66'sı (% 61.7) penisilin'e, 50'si (% 46.8) eritromisin'e, 42'si (% 39.3) amoksisiline dirençli olduğu tespit edildi.Öğe Mastitisli Süt İnerlerinden İzole Edilen Stafilokokların ß-Laktamaz Aktiviteleri ve Antibiyotiklere Duyalılıkları(2001) Hadimli, H. Hüseyin; Ateş, Mehmet; Güler, Leyla; Kav, Kürşat; Öncel, TanerBu çalışmada, mastitisli süt ineklerinden izole edilen 82 koagulaz pozitif (78 Staphylococcus aureus, 4 S. in-termedius) ve 25 koagulaz negatif (15 S. epidermidis, 4 S. simulans, 2 S. chromogenes, 2 S. sapmphyticus, 2 S. caprae) toplam 107 stafilokok suşunun \beta -laktamaz aktiviteleri ve antibiyotiklere duyarlılıkları belirlendi. Koagulaz pozitif sta-filokoklardan 36'sı (% 46.1) (S. aureus) ve koagulaz negatif suşların 12'si (% 48) (10 S. epidermidis, 2 S. simulans) \beta -laktamaz pozitif bulundu. Antibiyotik duyarlılık testlerine göre; 107 stafilokok suşunun 105'i (% 98.1) enrofloksasilin'e, da-nofloksasilin'e, amoksisilin klavulonik asit'e, 104'ü (% 97.1) sefaperazonsulbaktam'a, 98'i (% 91.5) kloksasilin'e, 93'ü (% 86.9) sefaperazon'a, 86'sı (% 80.3) oksasilin'e, 85'i (% 79.4) oksitetrasiklin'e, 83'ü (% 77.5) ampisilinsulbaktam'a, 80'i (% 74,7) gentamisin'e ve 76'sı (% 71.0) trimetoprim şulfametaksazol'a duyarlı bulundu. Ayrıca, stafilokok suşlarının 67'si (% 62.3) ampisilin'e, 66'sı (% 61.7) penisilin'e, 50'si (% 46.8) eritromisin'e, 42'si (% 39.3) amoksisiline dirençli olduğu tespit edildi.Öğe Tavuk ve Hindilerden Ornithobacterium Rhinotracheale İzolasyonu(2002) Erganiş, Osman; Ateş, Mehmet; Hadimli, Hasan Hüseyin; Çorlu, MehmetA total of 251 tracheal swabs and a total of 102 tracheae, lungs, airsacs, livers and spleens were collected from commercial layer, broiler and broiler parent stock flocks with respiratory infection. From turkey flocks, 96 tracheal swabs and 5 tissue samples from the trachea, lung, airsac, liver and spleen were also taken. Cultivations on Blood Agar supplemented with 5-10%sheep blood, on Chocolate Agar and on MacConkey Agar were incubated at 37\circC for 24-72 hours in the presence of 10%CO_2. Gram staining was carried out for the smooth and non-hemolytic colonies 1-3 mm in diameter and for those showing grayish pigmentation after approximately 48 hours of culture. Gram negative pleomorphic rods were considered to be Ornithobacterium rhinotracheale (ORT) and other conventional tests were made as follows: hemolysis, motility, spore formation, growth on MacConkey and TSI agars, catalase, indol, sorbitol, inositol, rafinose, trehalose, xylose, dulcitol, nitrate, methyl red, oxidase, b-D galactosidase, urease, glucose, galactose, lactose, maltose, fructose and Voges-Proskauer. ORT was isolated from none of the samples taken from turkeys, broiler or broiler parent stock flocks. However, two ORT isolations were achieved from two commercial pullets aged 12 and 15 weeks. One of these isolates was serotyped as "B" by the AGP test while the other was not found to belong to any of the serogroups. Both of the isolates were found to be susceptible to oflaxacin, erythromicin, lincomycin, amoxicillin, and amoxicillinclavulonic acid by antibiotic suspectibility tests.
