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    Analyses of fruit attributes by multidimensional scaling method of apple genetic resources from coastal zone of North Eastern Anatolia, Turkey
    (ELSEVIER SCIENCE BV, 2018) Dumanoglu, Hatice; Aygun, Ahmet; Delialioglu, Rabia Albayrak; Erdogan, Veli; Serdar, Umit; Kalkisim, Ozgun; Bastas, Kubilay
    North Eastern Anatolia had a significant contribution to development of cultivated apple. A lot of local apple genotypes have been successfully grown without any pesticide applications despite to high rainfall (900-2300 mm) and humidity (70-85%) especially at the coastal part of the Eastern Black Sea Region of North Eastern Anatolia. We have investigated and selected local apple genotypes from the region which could be of importance for apple breeding by fruit attributes. In this study, sensory characteristics (186 accessions) and quantitative characteristics (176 accessions) determined based on Apple Descriptor, and were analyzed by Multidimensional Scaling analysis. Harvest maturity dates classified the genotypes into early season (19), mid season (13) and late season (154) apples. Among the genotypes, 39.25% had medium-sized fruits, 53.23% had intermediate attractiveness, 50% had ground color of green-yellow, 59.68% had skin over color of red, 61.29% had good eating quality and 53.76% had fine flesh texture. Quantitative characters showed large variation that fruit weight ranged from 27.4 g to 246.4 g, SSC from 8.6% to 15.4%, TA from 0.08% to 2.24%. Multidimensional Scaling analysis indicated that the genotypes harvested in early and mid-season exhibited larger variation for sensory and quantitative characteristics than did late season apples. Although several genotypes were differentiated for investigated characteristics and/or variables in early and late season groups, mid-season apples did not tend to form any cluster.
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    Effectiveness of Host Resistance Inducers and Humic Acid for Fire Blight Control and Shoot Growth on Apple Cultivars with M9 Rootstock
    (AMER SOC HORTICULTURAL SCIENCE, 2009) Bastas, Kubilay
    [Abstract not Available]
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    Exploring the interaction between small RNAs and R genes during Brachypodium response to Fusarium culmorum infection
    (ELSEVIER SCIENCE BV, 2014) Lucas, Stuart James; Bastas, Kubilay; Budak, Hikmet
    The present study aims to investigate small RNA interactions with putative disease response genes in the model grass species Brachypodium distachyon. The fungal pathogen Fusarium culmorum (Fusarium herein) and phytohormone salicylic acid treatment were used to induce the disease response in Brachypodium. Initially, 121 different putative disease response genes were identified using bioinformatic and homology based approaches. Computational prediction was used to identify 33 candidate new miRNA coding sequences, of which 9 were verified by analysis of small RNA sequence libraries. Putative Brachypodium miRNA target sites were identified in the disease response genes, and a subset of which were screened for expression and possible miRNA interactions in 5 different Brachypodium lines infected,with Fusarium. An NBS-LRR family gene, 1g34430, was polymorphic among the lines, forming two major genotypes, one of which has its miRNA target sites deleted, resulting in altered gene expression during infection. There were siRNAs putatively involved in regulation of this gene, indicating a role of small RNAs in the B. distachyon disease response. (C) 2013 Elsevier B.V. All rights reserved.