Yazar "De Luca, Elisa" seçeneğine göre listele

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    Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent
    (TAYLOR & FRANCIS LTD, 2017) Campestre, Cristina; Locatelli, Marcello; Guglielmi, Paolo; De Luca, Elisa; Bellagamba, Giuseppe; Menta, Sergio; Zengin, Gökhan
    This paper reports the MEPS-HPLC-DAD method for the simultaneous determination of 12 azole drugs (bifonazole, butoconazole, clotrimazole, econazole, itraconazole, ketoconazole, miconazole, posaconazole, ravuconazole, terconazole, tioconazole and voriconazole) administered to treat different systemic and topical fungal infections, in biological samples. Azole drugs separation was performed in 36 min. The analytical method was validated in the ranges as follows: 0.02-5 mu g mL(-1) for ravuconazole; 0.2-5 mu g mL(-1) for terconazole; 0.05-5 mu g mL(-1) for the other compounds. Human plasma and urine were used as biological samples during the analysis, while benzyl-4-hydroxybenzoate was used as an internal standard. The precision (RSD%) and trueness (Bias%) values fulfill with International Guidelines requirements. To the best of our knowledge, this is the first HPLC-DAD procedure coupled to MEPS, which provides the simultaneous analysis of 12 azole drugs, available in the market, in human plasma and urine. Moreover, the method was successfully applied for the quantitative determination of two model drugs (itraconazole and miconazole) after oral administration in real samples.
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    Analytical Procedures for Secondary Metabolites Determination: Recent Trends and Future Perspectives
    (BENTHAM SCIENCE PUBL LTD, 2018) Menghini, Luigi; Bellagamba, Giuseppe; Fabiana, Marcelli; De Luca, Elisa; Armellani, Luca; Cifelli, Roberta; Granese, Arianna
    Background: Many studies have shown the benefits deriving from plants secondary metabolites to human health also for the prevention of several diseases associated to oxidative stress including cancer, cardiovascular diseases and diabetes mellitus. Nowadays there is a high attention on plant secondary metabolites and analytical techniques related to their identification and characterization. Conclusion: This review aims to report analytical procedures, instrumental configurations, and extraction approaches devoted to secondary metabolites analyses. In this direction, an introduction to secondary metabolites is made with emphasis on their formation, biological and pharmaceutical roles. Extraction, characterization and analytical procedures are described in the second part of this review, while a comparison of the analytical techniques will be discussed in the third part. This review could provide a better understanding of the analytical procedure and techniques for secondary metabolites and their applications in the scientific and industrial areas including food, food supplements, cosmetic, and pharmaceutical, especially related to quality control analyses and to chemical fingerprint elucidation, all in order to standardize these plant-derived extracts before in vitro and in vivo studies.
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    Comparative study of biological activities and multicomponent pattern of two wild Turkish species: Asphodeline anatolica and Potentilla speciosa
    (TAYLOR & FRANCIS LTD, 2016) Zengin, Gökhan; Menghini, Luigi; Malatesta, Luciano; De Luca, Elisa; Bellagamba, Giuseppe; Uysal, Şengül; Aktümsek, Abdurrahman
    The multicomponent pattern and biological characterization of plant material are essential for pharmaceutical field, in the food supplements quality control procedures and to all plant-based products. These nutrients often show valuable effects related to their consumption due to the occurrence of secondary metabolites that show useful properties on health. In this framework, researches performed on this topic play a central role for human health and drug development process. The aim of this study was to compare phenolics and free anthraquinones multicomponent pattern of two wild Turkish species: Asphodeline anatolica and Potentilla speciosa using validated high-performance liquid chromatography-photogiode array (HPLC-PDA) assays, coupled to biological evaluation. Even if some variances related to biological and enzymatic inhibition activities can be ascribed to other phytochemicals, the reported data support traditional use of Asphodeline anatolica and Potentilla speciosa roots as valuable natural font for the development of novel natural-derived drug formulations and/or food supplements with health and nutritional benefits.
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    Cytotoxic and Enzyme Inhibitory Potential of Two Potentilla species (P-speciosa L. and P-reptans Willd.) and Their Chemical Composition
    (FRONTIERS MEDIA SA, 2017) Uysal, Şengül; Zengin, Gökhan; Locatelli, Marcello; Bahadori, Mir B.; Mocan, Andrei; Bellagamba, Giuseppe; De Luca, Elisa
    In this work, the biological and chemical fingerprints of three extracts (ethyl acetate, methanol, and water) from two Potentilla species (Potentilla reptans and P. speciosa) were investigated. Antioxidant, enzyme inhibitory, and cytotoxic activities were performed for the biological fingerprint. For the chemical characterization, total bioactive components, and individual phenolic components were determined using photometric and HPLC methods, respectively. The main identified phenolic compounds in these extracts were rutin and catechin. Methanol and water extracts contained the highest total phenolic and flavonoid content. The results of antioxidant assays showed that methanol and water extracts displayed higher antioxidant activity compared to the ethyl acetate extract. Generally, methanol and water extracts exhibited higher biological activities correlated with higher levels the bioactive components. For P. speciosa, the methanol extract exhibited the highest enzyme inhibitory activity (except BChE inhibitory activity). P. reptans exhibited also high antiproliferative activity against MCF-7 cells whilst P. speciosa had weak to moderate activity against both of A549 and MCF-7 cell lines. The results suggest that Potentilla species could be potential candidates for developing new phyto-pharmaceuticals and functional ingredients.
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    Multicomponent pattern and biological activities of seven Asphodeline taxa: potential sources of natural-functional ingredients for bioactive formulations
    (TAYLOR & FRANCIS LTD, 2017) Locatelli, Marcello; Zengin, Gökhan; Uysal, Ahmet; Carradori, Simone; De Luca, Elisa; Bellagamba, Giuseppe; Aktümsek, Abdurrahman
    The current study was carried out to evaluate multicomponent pattern, biological and enzymatic activities of seven Asphodeline taxa root extracts as useful ingredients, due to the fact that these plants are commonly used as traditional food supplements in Turkish regions. The extracts were characterized for free anthraquinones and phenolics to obtain a specific chemical fingerprint useful for quality control. These analyzes were coupled to biological and enzymatic activities in order to obtain comprehensive information of the natural product. Free anthraquinones and phenolics were determined using validated HPLC-PDA methods. Antioxidant properties were determined by different procedures including free radical scavenging, reducing power, phosphomolybdenum and metal chelating assays. Ames assay was performed to evaluate mutagenic/antimutagenic properties. Enzyme inhibitory activities were tested against cholinesterase, tyrosinase, alpha-amylase and alpha-glucosidase. From the herein reported results, Asphodeline could be valuable for the production of bioactive products or food supplements for cosmetic and pharmaceutical industries.