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    Onosma aucheriana: A source of biologically active molecules for novel food ingredients and pharmaceuticals
    (ELSEVIER, 2015) Maskovic, Pavle Z.; Diamanto, Lazari D.; Vujic, Jelena M.; Cvetanovic, Aleksandra D.; Radojkovic, Marija M.; Gadiuric, Slobodan B.; Zengin, Gökhan
    The phenolic profile, antioxidant activity and cytotoxicity of O. aucheriana aqueous extract were investigated. HPLC-DAD (high performance liquid chromatography-diode array detector) technique was used to define the profile of phenolic compounds of the resultant extract. Antioxidant properties were evaluated using different methods, including phosphomolybdenum, free radical scavenging and lipid peroxidation assays. Rosmarinic, gallic and p-hydroxybenzoic acids were determined as the major phenolic compounds. Human rhabdomyosarcoma (RD), human cervix carcinoma (Hep2c) and murine fibroblast (L2OB) cell lines were used to examine the cytotoxicity of the extract. The IC50 (inhibitory concentration at 50%) values ranged from 25.54 to 40.34 mu g/mL. These results suggest that this species may be considered as a valuable candidate for preparing new food and drug formulations. (C) 2015 Elsevier Ltd. All rights reserved.
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    Optimization of the Extraction Process of Antioxidants from Orange Using Response Surface Methodology
    (SPRINGER, 2016) Maskovic, Pavle Z.; Diamanto, Lazari D.; Cvetanovic, Aleksandra; Radojkovic, Marija; Spasojevic, Miroslav B.; Zengin, Gökhan
    Different extraction conditions may significantly influence extraction of particular compound groups. In the present study, response surface methodology (RSM) based on Box-Behnken design was used to define the best combination of extraction temperature (20-60 A degrees C), ethanol concentration (10-90 %) and extraction time (60-180 min) for maximum yield of antioxidant compounds and maximum antioxidant activity of orange extracts. Experimental values of total phenol yields were in the range from 3.10 to 3.72 mgGAE/ml, while total flavonoid content was in the range from 1.42 to 2.13 mgRE/ml. Antioxidant activity expressed as the 50 % inhibition concentration (IC50 value) was in the range from 0.03 to 0.04 mg/ml. The experimental results were fitted to a second-order quadratic polynomial model, and they have shown a good fit to the proposed model (R (2) > 0.90). Determined optimized conditions for maximizing yield of antioxidant compounds were within the experimental range.