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Öğe Antibacterial effect of chlorhexidine-cetrimide combination, Salvia officinalis plant extract and octenidine in comparison with conventional endodontic irrigants(JAPANESE SOC DENTAL MATERIALS DEVICES, 2016) Guneser, Mehmet Burak; Akbulut, Makbule Bilge; Eldeniz, Ayce UnverdiThe aim of the present study was to compare the antimicrobial effect of sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), a CHX/cetrimide solution (CHX+CTR), octenidine hydrochloride (OCT) and Salvia officinalis plant extract against Enterococcus faecalis. Seventy decoronated single -rooted human teeth were infected and divided into 6 test (n,=10) and 2 control groups (n=5) (negative, sterile samples and positive, infected samples). Following irrigants were then applied to test groups: 2.5% NaOCl, 5.25% NaOCl, CHX, CHX+CTR, S. officinalis extract and OCT. The dentin chips were obtained from inner root canal walls and analyzed by counting the number of colony forming units (CFU). The 2.5% NaOCl, 5.25% NaOCl, CHX and OCT groups presented no bacterial growth (CFU=0). S. officinalis and CHX+CTR groups reduced the number of E. faecalis cells but could not eliminate all. OCT may have potential as an endodontic irrigant in treatment of infected root canals.Öğe Assessment of the sealing abilities of several root canal sealers and filling methods(TAYLOR & FRANCIS LTD, 2013) Ozcan, Erhan; Eldeniz, Ayce Unverdi; Aydinbelge, Hale AriObjective. To evaluate the sealing abilities of two root canal sealers (epoxy resin-based AH Plus (R) and polydimethylsiloxane-based GuttaFlow (R)) and of five root filling techniques (lateral condensation, matched taper single gutta-percha point, laterally condensed-matched taper gutta-percha point, Thermafil (R) and continuous wave of condensation), using a bacterial leakage model. Materials and methods. One hundred and seventy-four single-rooted human teeth were randomly divided into 10 test groups (n = 15) and two control groups (n = 12). The roots that were filled with the test material, using the different root filling techniques, were mounted in a two-chamber bacterial leakage model and Enterococcus faecalis was added to the upper chambers. The lower chambers of all of the specimens were checked every day during the test period (100 days). The day of turbidity was recorded for each sample. Statistical analysis was performed using the Kaplan-Meier and log-rank tests. Results. There was no significant difference between the epoxy resin-based and the polydimethylsiloxane-based sealers, irrespective of the filling techniques used (p > 0.05). The continuous wave of condensation technique was found to be superior to the other techniques (p < 0.05). The difference between the other groups was insignificant (p > 0.05). Conclusions. AH Plus and GuttaFlow sealers showed similar levels of sealing ability. The continuous wave of condensation technique had the best sealing capability when compared to the other techniques.Öğe Comparative antifungal efficacy of light-activated disinfection and octenidine hydrochloride with contemporary endodontic irrigants(SPRINGER LONDON LTD, 2015) Eldeniz, Ayce Unverdi; Guneser, Mehmet Burak; Akbulut, Makbule BilgeThe aim of this study was to evaluate the antifungal effects of light-activated disinfection (LAD) in comparison with contemporary root canal irrigation solutions: sodium hypochlorite and 2 % chlorhexidine gluconate and a new wound antiseptic, octenidine hydrochloride. Seventy extracted teeth having single root canals were contaminated with Candida albicans for 14 days. The samples were divided into five experimental (n = 10) and two control (positive and negative) groups (n = 10): (1) LAD with toluidine blue O, (2) octenidine hydrochloride (OCT), (3) 2.5 % sodium hypochlorite (2.5 % NaOCl), (4) 5.25 % sodium hypochlorite (5.25 % NaOCl) and (5) 2 % chlorhexidine. Five millilitres of each test solution was applied for 3 min, and irradiation time used for LAD was 30 s. After treatment, the dentin chips were collected from inner canal walls into vials containing phosphate buffered saline, vortexed, serially diluted, seeded on Tryptic Soy Agar plates and incubated (37 A degrees C, 48 h). The number of colony-forming units was then counted. Differences between LAD group and positive control group were statistically significant (P < 0.05). All Candida cells were totally eliminated in root canals irrigated with OCT, 2.5 % NaOCl, 5.25 % NaOCl and 2 % chlorhexidine groups (CFU = 0). Within the limitations of this ex vivo study, LAD had minimal antimicrobial effect on C. albicans when used 30 s, and further modifications in LAD protocol are required to improve its antifungal capability. A new wound antiseptic, octenidine hydrochloride, demonstrated better potential than LAD in elimination of Candida albicans cells and may be a promising alternative to NaOCl and chlorhexidine solutions in future.Öğe A comparative evaluation of sealing ability of a new, self-etching, dual-curable sealer: Hybrid Root SEAL (MetaSEAL)(MOSBY-ELSEVIER, 2008) Belli, Sema; Ozcan, Erhan; Derinbay, Oznur; Eldeniz, Ayce UnverdiObjective. To assess the long-term sealing ability of a new dual-curable, self-etching, 4-META containing resin-based sealer: Hybrid Root SEAL (MetaSEAL in the United States) and compare with RealSeal and AH Plus sealers. Methodology. Root canals of 44 extracted and decoronated single-rooted human teeth were instrumented using a crown-down technique with ProFile 0.04 tapered NiTi rotary instruments to ISO size 30 and then to size 45 with K-hand files. Four roots were selected and used as positive and negative controls (n = 2), the rest were randomly divided into 4 groups (n = 10) and filled using 0.04 tapered size 45 cones as follows: Group 1: AH Plus with gutta-percha; Group 2: Hybrid Root SEAL (MetaSEAL) with gutta-percha; Group 3: Hybrid Root SEAL (MetaSEAL) with Resilon point; and Group 4: RealSeal with Resilon point. The quality of seal of each specimen was measured after 1, 4, 12, and 24 weeks using a fluid transport model. Measurements were made at 2-minute intervals for 8 minutes. The data were calculated as Lp and statistically analyzed using 2-way repeated measures of ANOVA and Bonferroni pairwise comparison tests (alpha = 0.05). Results. There were no significant differences among test materials in terms of fluid microleakage values (P = .126). There were statistically significant differences among the time periods (P = .009) and observed between 1- and 24-week test periods. There was significant interaction between root canal filling materials and time of testing (P = .048). Conclusion. Within the limitations of this in vitro study, it was concluded that recently introduced Hybrid Root SEAL (MetaSEAL) showed similar sealing performance with RealSeal and AH Plus sealers when used either with gutta-percha or Resilon at 24 weeks. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 106: e45-e52)Öğe Effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with Bioaggregate apical plugs(TAYLOR & FRANCIS LTD, 2016) Akman, Melek; Akbulut, Makbule Bilge; Guneser, Mehmet Burak; Eldeniz, Ayce UnverdiTo evaluate the effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with DiaRoot BioAggregate (BA) when used as apical plugs. Forty single-rooted teeth were prepared using Peeso reamers. The samples were divided into four groups. The intracanal medicaments were applied to the root canals as follows: Group1: a combination of metronidazole-ciprofloxacin-cefaclor, Group2: a combination of metronidazole-ciprofloxacin, Group3: calcium hydroxide, and Group4: no medication. After 21 days, the medicaments were removed. The apical part of each root was horizontally sectioned into 1-mm thick slices. The samples were divided into two subgroups, and the following materials were placed: Biodentine, DiaRoot-BioAggregate. After 48-h incubation, the push-out bond strength was measured. The data were analyzed by a two-way ANOVA. Biodentine showed a significantly higher mean push-out bond strength value than DiaRoot-BioAggregate (P=0.00). The medications have an effect on the push-out bond strength of both materials (P=0.002). Biodentine showed better adhesive performance as an apical plug than DiaRoot-BioAggregate.Öğe Effect of temporary filling materials on repair bond strengths of composite resins(WILEY-LISS, 2008) Erdemir, Ali; Eldeniz, Ayce Unverdi; Belli, SemaEndodontic access cavities sometimes can be prepared through a permanent composite restoration. Between the appointments, temporary cements are used to seal access cavities and may have negative effect on bonding of further composite restoration. The purpose of this study was to compare shear bond strength of composite to composite which had been in contact with various temporary filling materials. Standard cavities were prepared on 160 acrylic resin blocks, obturated with composite resin (Clearfil AP-X, Kuraray, Japan) and randomly divided into eight groups (n = 20). Group 1 received no treatment. From group 2-8, composite surfaces were covered with the following cements temporarily: Zinc-oxide/calcium-sulphate (Cavit-G, ESPE, Germany), two different Zinc-Oxide-Eugenol materials (ZnOE, Cavex, Holland and IRM, Dentsply, USA), Zinc-phosphate cement (Adhesor, Spofa-Dental, Germany),Zinc-polycarboxylate cement(Adhesor-Carbofine,Spofa-Dental, Germany), Glass-Ionomer-Cement (Argion-Molar, Voco, Germany), or light curing temporary material (Clip, Voco, Germany). The cements were removed mechanically after 1 week storage in distilled water at 37 degrees C and composite surfaces were treated with a self-etch adhesive system (SE-Bond, Kuraray, Japan). Composite resin build-ups were created on composite surfaces. Shear bond strength values were measured using universal testing machine at crosshead speed of 1 mm/min. The data was calculated in MPa and statistically analyzed using one-way ANOVA and Tukey tests. Eugenol-containing cements significantly reduced shear bond strengths of composite to composite (p < 0.05), while the other temporary materials had no adverse effect on shear bond strength (p > 0.05). These findings suggested that temporary filling materials except eugenol-containing materials have no negative effect on composite repair bond strengths. (c) 2007 Wiley Periodicals, Inc.Öğe Effect of Various Endodontic Irrigants on the Push-out Bond Strength of Biodentine and Conventional Root Perforation Repair Materials(ELSEVIER SCIENCE INC, 2013) Guneser, Mehmet Burak; Akbulut, Makbule Bilge; Eldeniz, Ayce UnverdiIntroduction: The aim of this study was to evaluate the effect of various endodontic irrigants on the push-out bond strength of Biodentine (Septodont, Saint Maur des Fosses, France) in comparison with contemporary root perforation repair materials. Methods: Midroot dentin of canine teeth was horizontally sectioned into 1-mm-thick slices. The canal space of each dentin slice was enlarged with a diamond bur to 1.4 mm in diameter. The samples were divided into 5 groups (n = 40), and the following materials were placed, respectively: Biodentine, ProRoot MTA (Dentsply Tulsa Dental, Tulsa, OK), amalgam, Dyract AP (Dentsply DeTrey, Konstanz, Germany), and intermediate restorative material (IRM, Dentsply DeTrey). The samples were wrapped in wet gauze for 10 minutes and divided into 3 subgroups (n = 10) to-be immersed into 3.5% sodium hypochlorite, 2% chlorhexidine gluconate (CHX), or saline for 30 minutes. No irrigation was performed in the controls (n = 10), and a wet cotton pellet was placed over each test material. After incubation for 48 hours, the dislodgement resistance of the samples was measured using a universal testing machine. The samples were examined under a stereomicroscope to determine the nature of the bond failures. Results: Biodentine showed significantly higher push-out bond strength than MTA (P < .05). The statistical ranking of push-out bond strength values was as follows: Dyract AP > amalgam >= IRM >= Biodentine > MTA. The push-out bond strength of Dyract AP, amalgam, IRM, and Biodentine was not significantly different when immersed in NaOCl, CHX, and saline solutions, whereas MTA lost strength when exposed to CHX. Conclusions: Biodentine showed considerable performance as a perforation repair material even after being exposed to various endodontic irrigants, whereas MTA had the lowest push-out bond strength to root dentin. (J Endod 2013;39:380-384)Öğe Effects of Novel Root Repair Materials on Attachment and Morphological Behaviour of Periodontal Ligament Fibroblasts: Scanning Electron Microscopy Observation(WILEY-BLACKWELL, 2016) Akbulut, Makbule Bilge; Arpaci, Pembegul Uyar; Eldeniz, Ayce UnverdiThe aim of this study was to evaluate the adhesion of periodontal ligament fibroblasts (PDLs) on newly proposed root repair materials [Biodentine, MM-MTA, polymethylmethacrylate (PMMA) bone cement, and SDR], in comparison with contemporary root repair materials [IRM, Dyract compomer, ProRoot MTA (PMTA), and Vitrebond]. Five discs from each material were fabricated in sterile Teflon molds, and the specimens were aged and prewetted in cell culture media for 96 hours. Three material discs were used for scanning electron microscopy (SEM) for the assessment of the attachment, density, and morphological changes in the PDLs, while two samples were used for energy dispersive x-ray spectroscopy (SEM-EDX) to determine the elemental composition of the materials. Human PDLs were plated onto the materials at a density of 10,000/well, and incubated for 3 days. The SEM micrographs were taken at different magnifications (5003 and 50003). In the SEM, the cells were attached and well spread-out on the surfaces of the Biodentine, PMTA, and Dyract compomer, while varied cell densities and morphological alterations were observed in the Vitrebond, IRM, MM-MTA, SDR, and PMMA bone cement groups. The SEM-EDX analysis revealed a maximum calcium percentage in the PMTA specimens, as well a maximum silicon percentage in the Dyract compomer specimens. This in vitro study demonstrated that the Biodentine and Dyract compomer supported PDL cell adhesion and spreading. The PMTA presented a favorable scaffold for better attachment of the PDL cell aggregates. Therefore, the calcium and silicon content of a material may enhance the PDL cell attachment.Öğe Evaluation of antibacterial effects of contemporary orthodontic bonding materials(TAYLOR & FRANCIS LTD, 2017) Baka, Zeliha Muge; Eldeniz, Ayce Unverdi; Guneser, Mehmet Burak; Basciftci, Faruk AyhanAim: The aim of this study was to evaluate the antibacterial effects of five orthodontic bonding materials commonly used in orthodontics. Materials and Methods: The antibacterial effects of five orthodontic bonding materials were evaluated against Streptococcus mutans and Streptococcus salivarius by direct contact test (DCT). Using the DCT technique, quintet specimens of Transbond XT, Blugloo, Grengloo, Light Bond, and Opal Bond were applied to the bottom and the walls of the five wells of a 96-microtiter plate with a height of 2 mm. Samples were prepared in two sets: 1-day samples and 7-day samples. Ten microliters of bacterial suspension were added to each well for direct contact with each material for 1h at 37 degrees C. Bacterial growth was then measured in a microplate spectrophotometer hourly at 600 nm for 24h. Five uncoated wells with identical inoculum sizes served as positive controls. The data obtained at the end of 24h was statistically analyzed with one-way ANOVA, and post hoc comparisons were done using Tamhane's T2 test. Results: Blugloo generally showed better antibacterial activity than the other materials. Transbond XT did not show any antibacterial activity. There was a statistically significant difference between Transbond XT and Light Bond in the 1-day sample against S. mutans (p<0.05). No statistically significant differences were found among the other groups (p>0.05). Conclusion: Blugloo had the most potent antibacterial activity against S. salivarious.Öğe Evaluation of pH and calcium ion release of Acroseal sealer in comparison with Apexit and Sealapex sealers(MOSBY-ELSEVIER, 2007) Eldeniz, Ayce Unverdi; Erdemir, Ali; Kurtoglu, Firuze; Esener, TimurObjective. This is an in vitro assessment of pH level and calcium ion release exhibited by 3 calcium hydroxide-based root canal sealers-Sealapex, Apexit, and Acroseal. Study design. The materials were prepared according to the manufacturers' instructions and placed in 1 cm long and 4 mm diameter tubes. The tubes were then immersed in a glass flask containing 10 mL bidistilled water (n = 15), which was sealed and stored at 37 degrees C before the materials had set. The control group contained bidistilled water with empty tubes (n = 12). At predetermined time intervals (24 h, 96 h, and 7, 15, and 28 days) the pH of the bidistilled water was tested with a pH meter and for released calcium by using spectrophotometry. The data were statistically analyzed using 1-way analysis of variance for the comparison of the materials at each time point. If the difference was significant, individual comparisons were performed by Tukey multiple comparisons test (alpha = .05). Results. Sealapex produced higher pH and released significantly higher calcium amounts than the other 2 sealers at all periods (P < .05). Apexit showed higher calcium release than Acroseal at the end of 15 days (P < .05). There was no significant difference in the pH between Apexit and Acroseal (P > .05). Conclusion. The new Acroseal sealer presented the least calcium ion release and pH than Sealapex and less calcium ion release than Apexit sealer.Öğe Fracture resistance of roots obturated with a novel calcium silicate-based endodontic sealer (BioRoot RCS)(TAYLOR & FRANCIS LTD, 2016) Guneser, Mehmet Burak; Akman, Melek; Kolcu, Inci Baser; Eldeniz, Ayce UnverdiThe aim of this ex vivo study was to evaluate the vertical-fracture resistance of roots obturated with a newly developed tricalcium silicate cement (BioRoot RCS; Septodont, Saint Maur Des Fosses, France) using cold lateral compaction technique (LC) or matched-taper single-cone gutta-percha technique (SC). Decoronated 82 single-rooted mandibular premolars were chemo-mechanically prepared and then randomly divided into 6 experimental groups (n = 12) and 2 control groups (n = 5): Group 1 iRoot SP-LC; Group 2 iRoot SP-SC; Group 3 MTA Fillapex-LC; Group 4 MTA Fillapex-SC; Group 5 BioRoot RCS-LC and Group 6 BioRoot RCS-SC. In the positive-control group, roots were instrumented but not filled, and in the negative-control group, roots were neither instrumented nor filled. All samples were incubated for two weeks and then subjected to vertical loading force (1 mm/min) until fracture. The force required to fracture each specimen were determined, and the data were statistically analyzed. The highest significant fracture resistance was recorded for the iRoot SP-LC, iRoot SP-SC, BioRoot RCS-LC, and BioRoot RCS-SC groups, with no significant difference among them (p > 0.05) when compared with the positive-control group (p < 0.05), whereas the lowest significant values were observed in the MTA Fillapex-LC and MTA Fillapex-SC groups (p < 0.05). The vertical fracture resistance of roots obturated with BioRoot RCS and iRoot SP sealers using either LC or SC technique was found to be similar to that of intact teeth. BioRoot RCS, newly developed tricalcium silicate cement, might have the potential to reinforce the instrumented teeth against vertical root fracture.Öğe In vitro antimicrobial activity of different electrochemically-activated solutions on enterococcus faecalis(ISTANBUL UNIV PRESS, ISTANBUL UNIV RECTORATE, 2019) Akbulut, Makbule Bilge; Eldeniz, Ayce UnverdiPurpose The aim of this in vitro study was to assess and compare the antimicrobial efficacy of different electrochemically-activated solutions (ECA) and contemporary irrigants, in root canals infected with Enterococcus faecalis, used with or without EndoActivator (EA). Materials and methods A hundred single-rooted human teeth were prepared. Ninety of the root segments were infected with E. faecalis for four weeks, and divided into eight test groups (n = 10) (four with and four without EA sonication) and a positive control (n = 10). The irrigants tested were electrochemically-activated solutions produced by the Medilox (R) (ECA-MX) and Envirolyte (R) devices (ECA-EN), 2% CHX and 2.5% NaOCl. The root specimens were irrigated with 5 mL of the test solution, with additional sonic agitation applied to the EA groups. The dentine samples that were obtained from the walls were cultured, and the antibacterial efficacy was evaluated by counting the colony-forming units. Results The ECA-EN, 2.5% NaOCl and 2% CHX were more effective than the ECA-MX (p < 0.05) with the addition of EA sonication, showing no statistical difference in the elimination of E. faecalis. Conclusion The ECA-EN shows potential as an endodontic irrigant, while EA usage gives no benefit in reducing bacteria from root canals.Öğe Push-out bond strength of a new post system after various post space treatments(JAPANESE SOC DENTAL MATERIALS DEVICES, 2016) Akman, Melek; Eldeniz, Ayce Unverdi; Ince, Selen; Guneser, Mehmet BurakTo assess the effect of post-space treatment with chelating agents on the push-out bond-strength of a glass fiber post-system. Fortyeight human teeth were decoronated. The roots were prepared to size 40 and obturated. The post-spaces were prepared with PeesoReamer drills. The post-spaces were irrigated with (i) NaOCl and EDTA with chlorhexidine (QMix), NaOCl and EDTA, NaOCl and Citric acid, (iv) NaOCl and saline as a control group. i-TFC glass fiber posts were then luted with i-TFC bond system. The samples were horizontally sectioned. The displacement resistance was measured. Push-out bond-strength (MPa) was calculated. Data were analyzed. NaOCl/QMix group showed highest values to dentine whereas NaOCl/Citric acid group showed lowest values. i-TFC post-system demonstrated equal bond strength values when post-space treated with either NaOCl/EDTA or NaOCl/Saline. It can be concluded that post-space could be treated with NaOCl and QMix in order to increase adhesion of i-TFC post-system to root dentine.Öğe Tooth discoloration effects of calcium silicate based barrier materials used in revascularization and treatment with internal bleaching(ELSEVIER TAIWAN, 2017) Akbulut, Makbule Bilge; Terlemez, Arslan; Akman, Melek; Buyukerkmen, Begum; Guneser, Mehmet Burak; Eldeniz, Ayce UnverdiBackground/purpose: Usage of barrier materials is an important step in revascularization procedure. One of the undesired properties of these barrier materials is to cause coronal tooth discoloration. The aim of this in vitro study was to evaluate the tooth discoloration induced by ProRoot MTA (PMTA), Biodentine, and MM-MTA, as well as the efficacy of internal bleaching on this discoloration. Materials and methods: Forty-two maxillary incisor teeth were prepared. Triple antibiotic paste (TAP) was placed in the root canals and incubated for 3 weeks. After removing the TAP, blood embedded spongostans were inserted into the root canals, and PMTA, Biodentine, or MM-MTA was placed over them. The teeth were incubated for 4 weeks at 37 degrees C; then, the internal bleaching agent was sealed for one week. The tooth color was measured throughout the study and the color change values (Delta E) of each specimen were calculated, and the data was statistically analyzed using the one-way ANOVA and Tamhane's T2 tests. Results: The TAP significantly decreased the luminosity of the teeth (p < 0.05); however, no significant differences were observed between the tooth discolorations induced by the PMTA, Biodentine, and MM-MTA (p > 0.05). The teeth in the Biodentine group were more whitened than those of the PMTA and MM-MTA groups (p < 0.05). Conclusion: Although the PMTA, Biodentine, and MM-MTA caused similar color alterations in the teeth, more bleaching was observed on those teeth discolored using TAP + blood + Biodentine. (C) 2017 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.
