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    Hepatoprotective effects of coriandrum sativum essential oil against acute hepatotoxicity induced by carbon tetrachloride on rats
    (University of Istanbul, 2016) Özbek H.; Kırmızı N.İ.; Cengiz N.; Erdoğan E.
    The aim of this study was to evaluate effect of Coriandrum sativum (CS) essential oil in rat model of carbon tetrachloride (CCl4) induced liver toxicity. Experimental groups were formed as follows: isotonic saline solution (ISS), silibinin, CS-1 (0,3ml/ kg), CS-2 (0,6 ml/kg). Agents were administered intraperitoneally. Blood and liver tissues were collected at the end of the study ended. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were measured. Liver tissues were evaluated histopathologically. One-way analysis of variance (ANOVA) was used for statistical analyses.As a result silibinin and CS-2 decreased blood AST and ALT levels of their groups and these biochemical results were supported by histopathological results. In conclusion this study has provided evidence that Coriandrum sativum essential oil has significant hepatoprotective effect on carbon tetrachloride induced liver toxicity in rats. © 2016, University of Istanbul. All rights reserved.
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    A new method in CNS (Central Nervous System) in vitro cultures in the mouse: Study of effectiveness
    (Veteriner Fakultesi Dergisi, 2010) Erdoğan E.; Öztürk G.; Rağbetli M.C.
    In this study; to evaluate the effectiveness of collagen coating method, using in the peripheral nervous system cultures, and its involving factors caused from manipulations in central nervous system (CNS) cultures was aimed. Via frontal approach, brains, transected from young Swiss albino mice, were taken into artificial cerebro-spinal fluid immediately and made blocks in agarose gel. With a vibration microtome, 200 ?m thickness horizontally live slices were taken in to the dishes filled with culture medium. Tissue sections were analyzed as two groups. In the group 1 (control): fresh slices were evaluated directly. In the group 2: sections were covered with collagen gel (Type I) and left in the incubator (5% CO2) for 3 days. These sections were dyed with calcein and propidium iodide for viability and non-viability and then observed with confocal laser scanning microscope. Images were captured digitally and examined. Since negative effects of high melting temperature of standard agar on the livability, using low melting agar to tissue blocking and high frequency - low speed vibrotome setting to cut were more preferably. In the 3 days cultures, viability/nonviability rates were indicated better values. It is concluded that, in the CNS slicing cultures, collagen coating method was an easier, effective, useful and alternative method to present techniques.