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Öğe Comparison of immunogenicities of 3 different inactivated Newcastle vaccines (Manisa, Solvay ve Intervet) using in Turkey(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY, 1999) Erganis, O; Ok, U; Coskun, B; Inal, F; Tifik, AMA total of 1134 laying hens (Hysex-Brown hibrid), 3 different groups bearing 378 laying hens at each group was used, in this study done to compare immunological effects of 3 different (Solvay, Intervet and Manisa) inactivated Newcastle vaccines with mineral oil adjuvant. Same vaccination program and rations were applied to chickens during the study. Inactivated Newcastle vaccines were used according to the prospectuse by subcutaneously at 17 th weeks before laying period. Blood samples were taken from 24 hens per group, by intracardiac punction, at 3 th weeks, 5 th weeks, 9 th weeks, 13 th weeks, 17 th weeks, 22 nd weeks, 27 th weeks, 32 nd weeks, 37 th weeks, 45 th weeks and 52 nd weeks after vaccination. All sera were tested by micro hemaglutinasyon-inhibition (mHI) test Antibody titers were recorded as log2 and statical analysis was calculated by varians analysis. No significant differences were found in antibody titers among the vaccines for first nine months period (until 32 nd weeks). In the last measurements (at 37 th and 45 th weeks), higher antibody titers were obtained in Manisa group. This vaccine (Manisa) found at international quality must to combine with other vaccines (such as IB, Gumboro, EDS 76, E. coli, Salmonella, ext.,) for widespread using at poultry flocks in Turkey.Öğe Comparison of rose bengal plate test antigens prepared from Brucella abortus, Brucella melitensis and Brucella suis(NATL VETERINARY RESEARCH INST, 2005) Erganis, O; Hadimli, HH; Solmaz, H; Corlu, MRose bengal plate test (RBPT) antigens from Brucella melitensis and Brucella suis S 2 were prepared and compared with RBPT antigen prepared from classical Brucella abortus S 99. A total of 54 sera samples, of which 7 were collected from humans with brucellosis and 47 from infected sheep, were studied by using RBPT and serum agglutination test (SAT). RBPT and SAT results showed that RBPT antigens prepared from Br. melitensis and Br. suis S 2 were compatible with RBPT antigen prepared from Br. abortus S 99. Therefore, we concluded that RBPT antigens can be prepared from Br. melitensis and Br. suis S 2 and used for epidemiological surveilance of human and sheep brucellosis.Öğe Detection of Brucella antibodies in sheep sera using dot-immunobinding assay and Rose Bengal plate agglutination test(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY, 1997) Gurturk, K; Ilhan, Z; Erganis, OIn this study, a dot-immunobinding assay (DIA) using nitrocellulose membrane was performed for the detection of Brucella antibodies in 206 sheep sera. Extract of Brucella abortus strain S99 obtained by heat treatment was used as antigen. The results were compared with those of Rose Bengal plate agglutination test (RBPAT). Of the 96 sera collected from the 8 aborted sheep flocks, 46 were positive by both tests and 17 were positive only in DIA. Of the 110 sera collected from slaughtered sheep, 13 were positive by both tests and 4 were positive only in DIA. Of the total 206 Sera tested, 147 were negative by RBPAT and only 126 were negative in DIA. All the sera giving positive reactions in RBPAT were also positive in DIA. it is concluded that DIA would be useful to apply for the detection of Brucella antibodies in sheep sera.Öğe Efficacies of liposome-encapsulated enrofloxacin against Staphylococcus aureus infection in Anatolian shepherd dog monocytes in vitro(M H SCHAPER GMBH CO KG, 2005) Bas, AL; Simsek, A; Erganis, O; Corlu, MThe aim of this study was to evaluate the intracellular activity of two types of liposome-encapsulated enrofloxacin (LE) compared with free enrofloxacin and non-treated control against Staphylococcus aureus, phagocytosed by monocytes in healthy Anatolian Shepherd dogs. Enrofloxacin was encapsulated with two different types of liposome in multilamellar large vesicles (MLV). Type A MLV were composed of 15 mg phosphatidylcholine and 35 mg cholesterol, Type B MLV were composed of phosphatidylcholine, cholesterol and enrofloxacin in a molar ratio of 1:1:1. Intracellular activity was estimated by comparing the numbers of bacteria surviving intracellularly in monocytes exposed to free enrofloxacin and LE for 4 h at the doses of 0.25, 0.5 and 1 mu g/ml, with those surviving intracellularly in untreated control monocytes. All three forms of enrofloxacin (free, Type A and B liposomes) increased the intracellular killing of S. aureus in a concentration dependent manner. Comparison of 1 mu g/ml Type B LE revealed that killing activity was significantly higher than those of other concentrations. The results showed that LE was superior in reducing the number of intracellularly located bacteria compared to the free drug and control. The beneficial effect of liposomal encapsulation is presumably due to the fact that both liposomes and bacteria are localized at the same spot in phagocytic cells.Öğe Evaluation of a combined vaccine against staphylococcal mastitis in ewes(NATL VETERINARY RESEARCH INST, 2005) Hadimli, HH; Erganis, O; Kav, K; Sayin, ZThe effectiveness of combined staphylococcal vaccine (CSV), prepared from Staphylococcus aureus and coagulase negative staphylococci (CNS: Staphylococcus epidermidis, S. simulans, S. saprophyticus), on staphylococcal mastitis in sheep was determined. The group of pregnant sheep was vaccinated subcutaneously into supramammary lymph node twice at 3 week interval. Similarly, sterile saline was administered to controls. Blood and milk samples were collected before and at days 21 and 51 after the vaccination. The titre of S. aureus and CNS antibodies in serum and whey was determined using a modified ELISA. Levels of staphylococcal antibodies in serum and whey of the vaccinated animals were significantly higher compared with the controls. Intramammary infection rate by S. aureus was lower in the CSV group (7.70%) compared with the controls (30.76%) While intramammary infection rate for CNS was 19.23% in the controls, no isolate was demonstrated in the CSV group. Therefore, the effectiveness of CSV was found to be useful to decrease intramammary infection rate of S. aureus and CNS in ewes.
