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Öğe BIOCONTROL OF BOTRYTIS CINEREA ON STRAWBERRY FRUIT BY PLANT GROWTH PROMOTING BACTERIA(PAKISTIAN AGRICULTURAL SCIENTISTS FORUM, 2011) Donmez, M. F.; Esitken, A.; Yildiz, H.; Ercisli, S.In the present study, a total of 186 bacterial strains isolated from various soil sources and plant species from Eastern Anatolia region in Turkey were evaluated for their ability to suppress gray mold (Botrytis cinerea Pers. ex Fr.) occurred on strawberry cv. Fern. Among 186 bacterial strains, 36 were found effective to inhibit of development B. cinerea under in vitro conditions, and thirteen of them which have greater inhibition zone were selected as biocontrol agent. These antagonistic strains were identified as Bacillus lentimorbus, B. megaterium, B. pumilis, B. subtilis, Enterobacter intermedius, Kurthia sibirica, Paenibacillus polymyxa and Pantoea agglomerans. The inhibition zones among bacteria against B. cinerea were found between 0.50 (Bacillus C6, Brevibacterium MFD-47 and Pantoea MFD-232) and 3.75 cm (Enterobacter MFD-81) in vitro. The strawberry fruits were inoculated with B. cinerea in laboratory trials and Bacillus MFDU-2 (14.41 mm) was found more effective to prevent mycelial development on strawberry fruits in comparison to the control (19.20 mm). In terms of conidia germination on strawberry fruits, the lowest disease incidence was observed in MFD-45 treatment (20.8%), while disease incidence rate for the control was 79.2%. It was shown that antagonistic bacterial strains inhibited B. cinerea and that they have a potential use in sustainable strawberry production.Öğe Molecular and morphological characterization of pomegranate (Punica granatum L.) genotypes sampled from Coruh Valley in Turkey(FUNPEC-EDITORA, 2014) Orhan, E.; Ercisli, S.; Esitken, A.; Sengul, M.The pomegranate is one of the oldest fruits that are traditionally consumed by the local inhabitants of the Coruh Valley, Turkey. In this study, the molecular and morphological characteristics of 19 promising pomegranate genotypes selected from the Coruh Valley were evaluated. For the morphological evaluation, 22 quantitative fruit characteristics were used. For the molecular evaluation, 47 random amplified polymorphic DNA (RAPD) primers were used for polymerase chain reaction analysis. The principle component analysis of 22 quantitative fruit characteristics revealed that fruit weight and skin color are dominant traits for genotype discrimination. The unweighted average distance cluster of fruit characteristics revealed 3 distinct groups. Among the 47 RAPD primers, 9 exhibited reliable polymorphic patterns, and generated a total of 63 RAPD bands, of which 49.2% were polymorphic. The similarity matrix showed that the highest (0.920) and lowest (0.556) genetic similarities occurred between the APS13 and APS28 genotypes and the APS12 and APS42 genotypes, respectively. We determined clear discrepancies between the morphological and molecular data; consequently, the differences obtained among genotypes for fruit characteristics did not support genetic relationships among genotypes. In conclusion, molecular data provided the most reliable results at the DNA level.