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Öğe Comparison of a Newly Developed Automated and Quantitative Hepatitis C Virus (HCV) Core Antigen Test with the HCV RNA Assay for Clinical Usefulness in Confirming Anti-HCV Results(AMER SOC MICROBIOLOGY, 2011) Kesli, Recep; Polat, Hakki; Terzi, Yuksel; Kurtoglu, Muhammet Guzel; Uyar, YavuzHepatitis C virus (HCV) is a global health care problem. Diagnosis of HCV infection is mainly based on the detection of anti-HCV antibodies as a screening test with serum samples. Recombinant immunoblot assays are used as supplemental tests and for the final detection and quantification of HCV RNA in confirmatory tests. In this study, we aimed to compare the HCV core antigen test with the HCV RNA assay for confirming anti-HCV results to determine whether the HCV core antigen test may be used as an alternative confirmatory test to the HCV RNA test and to assess the diagnostic values of the total HCV core antigen test by determining the diagnostic specificity and sensitivity rates compared with the HCV RNA test. Sera from a total of 212 treatment-naive patients were analyzed for anti-HCV and HCV core antigen both with the Abbott Architect test and with the molecular HCV RNA assay consisting of a reverse transcription-PCR method as a confirmatory test. The diagnostic sensitivity, specificity, and positive and negative predictive values of the HCV core antigen assay compared to the HCV RNA test were 96.3%, 100%, 100%, and 89.7%, respectively. The levels of HCV core antigen showed a good correlation with those from the HCV RNA quantification (r = 0.907). In conclusion, the Architect HCV antigen assay is highly specific, sensitive, reliable, easy to perform, reproducible, cost-effective, and applicable as a screening, supplemental, and preconfirmatory test for anti-HCV assays used in laboratory procedures for the diagnosis of hepatitis C virus infection.Öğe Distribution of ıntestinal parasites for age and gender in the 13 to 18 years age group at the Nigde Orphanage(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2010) Ozkalp, Birol; Celik, Battal; Kurtoglu, Muhammet Guzel; Kesli, RecepThe present study was aimed at the determination of the rate of infection with intestinal parasites in children cared for at the Nigde Orphanage, and the demonstration of the distribution of infection in these children for age and gender. Faecal samples collected from 110 children, aged 13 to 18 years, were examined by the native method using physiological saline. Of the 110 faecal samples examined, 41 (37.3%) contained intestinal parasites, and of the samples containing parasites, 25 (61%) contained helminths and 16 (39%) contained protozoa. The number of faecal samples identified to contain a single parasite species was 39 (95%), while 2 (5%) samples contained more than one parasite species. As regards the distribution of the intestinal parasites for gender, it was ascertained that of the 75 females 30 (40%) individuals and of the 35 males 11 (31.5%) individuals were infected with intestinal parasites. The difference between the two sexes was considered to be insignificant. Faecal examination revealed the presence of the following parasite species at the indicated rates: Ascaris lumbricoides (34.1%), Entamoeba histolytica (29.3%), Taenia saginata (17.1%), Giardia intestinalis (9.8%), Enterobius vermicularis (7.3%) Trichuris trichiura (2.4%). The present study demonstrated that the environment of the orphanage was favourable for autoinfection and the spread of infection by direct contact. Furthermore, it was determined that the rate of parasitic infection was higher in the age group of 13 to 14 years, compared to the age group of 15 to 18 years.Öğe INVESTIGATION OF THE SUSCEPTIBILITIES OF MYCOBACTERIUM TUBERCULOSIS COMPLEX STRAINS TO MAJOR ANTITUBERCULOSIS DRUGS WITH BACTEC MGIT 960 SYSTEM(NOBEL ILAC, 2011) Kurtoglu, Muhammet Guzel; Kesli, Recep; Terzi, Yuksel; Baykan, MahmutObjective: The study was designed to investigate retrospectively the resistance rates of tuberculosis-causing mycobacteria, isolated in the microbiology and clinical microbiology laboratories of Konya Research and Education Hospital. Material and Method: Mycobacterium tuberculosis complex strains were isolated from various clinical samples of 1666 patients applying to Konya Research and Education Hospital between May 2007 and December 2009, and the resistance rates of Mycobacterium tuberculosis complex strains against first generation anti tuberculosis drugs were investigated. After homogenization and decontamination, the samples investigated were cultured using BACTEC Mycobacteria Growth Indicator Tube-960 (MGIT-960) system. Susceptibility rates of the strains determined with production were investigated with the same system versus streptomycin (SM), Isoniazid (INH), Rifampin (RIF) and Ethambutol (ETB) (SIRE). Results: From 1666 patients prediagnosed with tuberculosis, 70 Mycobacterium tuberculosis complex strains were isolated. While no drug resistance was found in 17 (24%) of them, resistance to one or two drugs was found in 26 (37%) strains (24% to INH, 20% to SM, 6% to ETB and 4% to RIF). While resistance was found to be only against one drugs in 15 of these (21%), two drugs in 11 of these (16%), no resistant strains could be determined against three or four drugs together Among the patients with resistance, 81% (57/70) displayed primary and 18% (13/70) secondary tuberculosis, and 2 patients were found to display resistance to isoniazid and rifampin together (MDR-TB). Conclosion: It was seen that the findings in the study were consistent with those determined by other studies in our country. Preventing the resistance to antituberculosis drugs is possible by enlightening the distribution rates of drug resistant strains in public, defining appropriate drug regimes and increasing the quality of tuberculosis control programs. Therefore, it is essential that regular and continuous scanning of antituberculosis drugs should be performed.Öğe Isolation of citrobacters in various infections and their antimicrobial sensitivity rates(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2011) Kurtoglu, Muhammet Guzel; Opus, Aysegul; Ozdemir, Mehmet; Baysal, BulentThe samples for culture from the patients admitted to Konya Research and Education Hospital between January 2009 and July 2010 were investigated. The obtained Citrobacter strains were studied for antimicrobial sensitivity rates and their distribution ratesto the clinics. The samples obtained were inoculated onto related culture media. Aseptic body samples were incubated at 37 degrees C into bottles of BACTEC 9120 blood culture system. For the identification and antimicrobial sensitivities of yielding bacteria, panels of Phoenix-100 automized identification device were used. Mean age rate of patients in whom Citrobacter strains were determined was 41.29 +/- 4.345. Of all samples with Citrobacter strains, 48% were isolated from urine, 29% from surgical wounds, 11% from sputum, 2% from peritoneal fluid, and 2% from vaginal samples. Of total 52 Citrobacter strains, the species level distribution was 46% C. freundii, 21% C. youngae, 15% C. koseri, 10% C. braakii, 6% C. farmeri and 2% C. wermanii. The distribution of samples with Citrobacter strains to the clinics were 29% adult intensive care unit (ICU), 21% pediatric, 11% general surgery, 10% in neonatal ICU, 10% plastic surgery, 7% urology, 6% burn unit, and 6% nephrology department. The most sensitive antimicrobial agents to Citobacter strains were amikacin (100%), meropenem (100%), imipenem (96%) and piperacillin/tazobactam (96%).Öğe Isolation Rate of Mycobacterium tuberculosis Complex from Patients with Suspected Tuberculosis and Identification of the Strains with BACTEC (TM) NAP and Immunochromatographic TB Ag MPT64 Rapid (TM) Tests(ANKARA MICROBIOLOGY SOC, 2011) Kurtoglu, Muhammet Guzel; Ozdemir, Mehmet; Kesli, Recep; Ozkalp, Birol; Baysal, BulentTuberculosis (TB) which is still one of the important infectious diseases in the world as well as Turkey, results in high morbidity and mortality. Clinical mycobacteriology laboratories have crucial roles in the identification, typing and susceptibility testing of Mycobacterium tuberculosis. The aims of this study were the investigation of the isolation rate of M.tuberculosis complex (MTC) from the clinical specimens of TB-suspected patients and to compare identification of mycobacteria isolated from solid and/or liquid media by using BACTEC NAP and immunochromatographic TB Ag MPT64 rapid test. A total of 1670 patients who were admitted to outpatients clinics of our hospital and prediagnosed as TB, have been included in the study. All the patients were anti-HIV seronegative. NALC-NaOH method were used for decontamination/homogenization, and preparations from samples were stained with Erlich-Ziehl-Neelsen method to detect acid-resistant bacilli (ARB) in direct microscopy. All of the samples were inoculated into BACTEC (TM) MGIT-960 (Becton Dickinson, USA) and Lowenstein-Jensen (14) media for cultivation and incubated at 37 degrees C for 6-8 weeks. Mycobacteria that were grown in the media have been identified by BACTEC (TM) NAP (Becton Dickinson, USA) and TB Ag MPT64 rapid test (SD Bioline Ag MPT64 Rapid (TM); Standard Diagnostics, Korea). The culture positivity in the samples of TB-suspected patients was found to be 3.7% (63/1670) with LJ and/or MGIT-960 methods, whereas ARB positivity rate was 1.6% (28/1670). Fifty-three (84%) out of culture positive 63 samples have been identified as MTC by BACTEC NAP test, while 61(97%) were found as MTC by TB MPT64 test. Considering BACTEC NAP test as the reference method, TB MPT64 test identified all the MTC strains correctly (sensitivity: 100%), however the false positivity rate was estimated as 12.7% (specificity: 87%). Of 53 MTC positive samples, 36 were sputum, four were bronchoalveolar lavage, four were urine, three were gastric fluid, three were pleural fluid, and one of each were abscess, peritoneal fluid and cerebrospinal fluid samples. ARB positivity rate was detected as 41.5% (22/53) among MTC culture positive samples. Of the patients who were infected with MTC, 72% (38/53) were male and 98% (52/53) were adults (age range: 20-85 years). Our data indicating 3.1% (53/1670) isolation rate of MTC from TB-suspected patients in our region were in concordance with the other results reported from Turkey. In conclusion, immunochromatographic TB Ag MPT64 test which seemed to be useful for the rapid identification of mycobacteria grown on solid and/or liquid, was practical to perform and had high sensitivity, however further larger-scaled studies are needed to support our data in our country.
