Yazar "Leporini, Lidia" seçeneğine göre listele

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    A comparative assessment of biological effects and chemical profile of Italian asphodeline lutea extracts
    (MDPI, 2018) Melucci, Dora; Locatelli, Marcello; Locatelli, Clinio; Zappi, Alessandro; De laurentiis, Francesco; Carradori, Simone; Campestre, Cristina; Leporini, Lidia; Zengin, Gökhan; Picot, Carene Marie Nancy; Menghini, Luigi; Mahomoodally, Mohamad Fawzi
    The present study aims to highlight the therapeutic potential of Asphodeline lutea (AL), a wild edible plant of the Mediterranean diet. Roots, aerial parts, and flowers of AL at two different phenological stages were collected from three locations in Italy. The inhibitory activities of extracts on strategic enzymes linked to human diseases were assessed. The antioxidant properties were evaluated in vitro, using six standard bioassays. The phenolic and anthraquinone profiles were also established using HPLC-PDA. Zinc, cadmium, lead, and copper contents were also determined. All the samples inhibited acetylcholinesterase (from 1.51 to 2.20 mg GALAEs/g extract), tyrosinase (from 7.50 to 25.3 mg KAEs/g extract), and alpha-amylase (from 0.37 to 0.51 mmol ACAEs/g extract). Aloe-emodin and physcion were present in all parts, while rhein was not detected. The phenolic profile and the heavy metals composition of specimens gathered from three different regions of Italy were different. It can be argued that samples collected near the street can contain higher concentrations of heavy metals. The experimental data confirm that the A. lutea species could be considered as a potential source of bioactive metabolites, and its consumption could play a positive and safe role in human health maintenance.
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    Crocus Sativus, Serenoa Repens and Pinus Massoniana Extracts Modulate Inflammatory Response in Isolated Rat Prostate Challenged with LPS
    (BIOLIFE SAS, 2017) Chiavaroli, Annalisa; Recinella, Lucia; Ferrante, Claudio; Locatelli, Marcello; Carradori, Simone; Macchione, Nicola; Zengin, Gökhan; Leporini, Lidia; Leone, Sheila; Martinotti, S.; Brunetti, L.; Vacca, M.; Menghini, Luigi; Orlando, Giulia
    Prostatitis is a common prostate disease that could be promoted by bacterial or non-bacterial infectious agents. In addition, inflammatory pathways involved in prostatitis have been increasingly studied, and herbal extracts endowed with anti-inflammatory effects are under investigation, individually or in combination, for their efficacy in alleviating the burden of inflammation, with possible improvements in symptoms. Serenoa repens (Serenoa), in combination with Crocus sativus (Crocus) and Pinus massoniana (Pinus), has previously shown to improve sexual function and limit urinary symptoms in patients suffering from concomitant erectile dysfunction and lower urinary tract symptoms. In this context, the aim of the present study is to evaluate the efficacy of Serenoa, Crocus and Pinus extracts, either alone or in combination, on immortalized prostate cells (PC3) and in an experimental model of bacterial prostatitis constituted by ex vivo prostate specimens challenged with lipopolysaccharide (LPS). We found that the tested extracts were able to reduce ROS production by PC3 cells and NF kappa B and PGE(2) activity in prostate specimens challenged with LPS. In addition, the pharmacological association of the extracts displayed synergistic effects indicating a rational use of the mixture of the tested extracts as a novel anti-oxidant and anti-inflammatory formulation in bacterial prostatitis. Finally, we performed analytical and in vitro evaluation to better characterize the phytochemical profile and the mechanism of action of selected secondary metabolites.
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    Multiple pharmacological approaches on hydroalcoholic extracts from different parts of Cynoglossum creticum Mill. (Boraginaceae)
    (TAYLOR & FRANCIS LTD, 2019) Menghini, Luigi; Ferrante, Claudio; Zengin, Gökhan; Mahomoodally, Mohamad Fawzi; Leporini, Lidia; Locatelli, Marcello; Cacciagrano, Francesco; Recinella, Lucia; Chiavaroli, Annalisa; Leone, Sheila; Brunetti, Luigi; Orlando, Giustino
    Cynoglossum creticum Mill (Boraginaceae) is used traditionally as a remedy to manage several human ailments. In this context, the present study aimed to perform multiple pharmacological investigations on the hydroalcoholic extracts prepared from Cynoglossum roots and aerial parts (leaves and flowers). We evaluated the antioxidant and enzyme inhibitory (against cholinesterases, alpha-glucosidase, alpha-amylase, lipase and tyrosinase) activity of the extracts. The protective effect(s) of the extracts on cardiomyocyte C2C12 and intestinal HCT116 cell lines challenged with hydrogen peroxide (H2O2) was studied. We found that the aerial parts harbored the highest amount of phenolic compounds. Generally, aerial parts showed significant antioxidant and enzyme inhibitory effects. Leaves exhibited the best lipase inhibitory activity (173.15 mgOE/g extract), followed by flowers and roots. The root and aerial extracts were equally able to blunt intracellular H2O2 induced reactive oxygen species production from both C2C12 and HCT116 cell lines. Both cells lines could be treated with scalar concentrations of root and flower extracts in the range 50-300 mu g/mL without interferences on cell viability. In conclusion, the present study showed protective effects exerted by Cynoglossum extracts, which could serve as a foundation for the development of pharmaceuticals and nutraceuticals derived from Cynoglossum.
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    New pharmacological targets of three Asphodeline species using in vitro and ex vivo models of inflammation and oxidative stress
    (TAYLOR & FRANCIS LTD, 2019) Zengin, Gökhan; Locatelli, Marcello; Ferrante, Claudio; Menghini, Luigi; Orlando, Giustino; Brunetti, Luigi; Recinella, Lucia; Chiavaroli, Annalisa; Leone, Sheila; Leporini, Lidia; Aumeeruddy, Muhammad Zakariyyah; Mahomoodally, Mohamad Fawzi
    This study explored the efficacy of the methanolic extract of three Asphodeline species (A. damascena subsp. rugosa, A. tenuior subsp. tenuiflora var. tenuiflora, and A. cilicica) to protect against hydrogen peroxide (H2O2)-induced lactate dehydrogenase (LDH) activity in HCT116 cells, and also any protective effects against lipopolysaccharides (LPS)-induced nitrite levels, prostaglandin E2 (PGE2) and 8-iso-prostaglandin F2 alpha (8-iso-PGF2 alpha) levels, 5HIAA/5-HT ratio, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 gene expression in rat colon specimens. Interestingly, A. tenuior extract was most effective in improving the tested biomarkers, by reducing LDH activity and nitrite level. On the other hand, A. damascena was the only species able to blunt LPS-induced TNF-alpha gene expression in rat colon specimens. The present findings highlighted the protective effects of Asphodeline extracts via in vitro and ex vivo models of inflammation and oxidative stress, adding new insights to the pharmacological actions of these medicinal plant species.