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Öğe Acute oral toxicity of Nerium oleander distillate in rats(WILEY-BLACKWELL, 2012) Dik, B.; Uney, K.; Ozdemir, O.; Demirci, S.; Yazihan, N. A.; Bas, A. L.[Abstract not Available]Öğe Detection of argyrophil nucleolar organizer regions (AgNORs) and proliferating cell nuclear antigen (PCNA) in epithelial skin tumours from domestic animals(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2009) Hatipoglu, F.; Ozdemir, O.; Kiran, M. M.The objective of this study was to compare the chemical characterization of the AgNOR bodies and the immunohistochemical detection of the PCNA for differentiating benign and malignant epithelial tumours (n = 21) front various animal Species (mainly dogs and cattle). Fibropapillomas / papillomas (n = 3) and fibropapillomatosis / papillomatosis (n = 3) in cattle. and perianal gland adenomas (n = 3), keracanthomas (n = 2) and epithelioma (n = 1) in dogs constituted the group of benign tumours whereas the identified malignant tumours were squamous cell carcinomas (n = 7) in cattle and in one sheep and carcinomas in dogs (n = 2). A complete AgNOR characterization with determination of the NAI (nuclear area index), the AgNAI (AgNOR area index). the AgNCI (AgNOR count index). the AgNAI/NAI (relative AgNOR area), the AgNAI/AgNCI (mean AgNOR area) and the AgNCI/NAI (A,,NOR density) was performed and PCNA expression was, explored in parallel by immunohistochemistry. Significant increases of AgNAI, AgNCI and AgNAI/NAI were evidenced in malignant tumours compared to benign ones whereas no significant difference of the PCNAI (numbers of PCNA positive cells) was found. Significant and positive correlations found between NAI and AgNAI or mean AgNOR area and between AgNAI and the mean AgNOR area or the relative AgNOR area in both malignant and benign tumours suggest that the AgNOR size is an important parameter for cell proliferation. By contrast, the lack of correlation between AgNCI and the relative AgNOR area and between the AgNOR density and NAI or AgNCI or AgNAI/AgNCI in malignant tumours contrary to benign tumours would be Clue to the under-estimation of the enumeration of the AgNOR bodies in malignant tumours mainly regrouped as AgNOR clusters.Öğe Effect of laminarin and chitosan gel formulations on the treatment of hydrofluoric acid induced corneal burns in the rabbits(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2008) Hatipoglu, F.; Ogurtan, Z.; Sezer, A. D.; Uney, K.; Erol, M.; Ozdemir, O.; Bas, A. L.Corneal burns were induced in 36 New Zealand white rabbits by instillation in both eyes of 0.05 mL of 2% hydrofluoric acid (HF) for 60 seconds. Following this, the eyes were irrigated with 500 mL isotonic saline (ISOT) and then the rabbits were divided into 4 treatment groups (1) = 9 rabbits each) including: laminarin solution (LS), chitosan hydrogel (CHG), chitosan hydrogel containing laminarin (CHG+L) and ISOT as the control. For each treatment group, one drop of each regimen was instilled 2 times a day and for periods of 2, 7 and 14 days respectively. Thus, 3 rabbits were used for each treatment period in each treatment group. The eyes were clinically examined immediately after the chemical burning and at days 1, 2, 7 and 14 of the follow up periods. The animals were euthanatized at the end of the follow-up periods and eyes were processed for histopathological examination. Clinical and histopathological results revealed that while LS or ISOT was effective, CHG and CHG+L were not effective in the treatment of HF corneal burns. LS had a better therapeutic effect than ISOT. CHG and CHG+L treatment had no accelerating effect on the healing of: corneal erosion throughout the experimental procedures.Öğe Effects of Tarantula cubensis alcoholic extract and Nerium oleander distillate on experimentally induced colon cancer(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2019) Er, A.; Ozdemir, O.; Coskun, D.; Dik, B.; Bahcivan, E.; Faki, H. Eser; Yazar, E.The aim of this research was to determine the effects of Tarantula cubensis alcoholic extract (TCAE) and Nerium oleander distillation (NOD) on the colon aberrant cryptal foci (ACF) score, serum cytokines [Tumour necrosis factor (TNF)-alpha, interleukin (IL)-2, IL-6, IL-10], prostaglandin E2, and oxidative status parameters (malondialdehyde, superoxide dismutase, catalase, glutathione peroxidase) in an experimentally induced colon cancer model in rats. Male rats (38 in total) were divided into 4 groups: Control group (n = 8), Colon Cancer group (CC, n = 10), CC+TCAE group (n = 10), and CC i NOD group (n = 10). Except for the Control group, the rest of rats received azoxymethane (15 mg/kg, SC) once a week for two weeks to induce colon cancer. TCAE was administered once a week at 0.2 mL/kg (SC) and NOD was administered in water to CC+TCAE and CC+NOD groups for 18 weeks. After blood samples were collected, the rats were euthanized, colons were removed, washed with saline, then fixed with 10% formaldehyde, and stained with 0.2% methylene blue. ACFs were scored by light microscopy. Serum levels of cytokines, prostaglandin E2, and oxidative status parameters were determined with ELISA. ACF score of the CC group was higher than CC+TCAE and CC+NOD groups. The prostaglandin E2 level of the Control group was lower than the CC i TCAE group, while IL-2 and IL-10 levels of the Control group were lower than all other groups. In conclusion, it can be stated that TCAE and NOD applications may be beneficial in the treatment of colon cancer.Öğe The prevalence of Familial Mediterranean Fever common gene mutations in patients with simple febrile seizures(VERDUCI PUBLISHER, 2014) Ozen, F.; Kocak, N.; Kelekci, S.; Yildirim, I. H.; Hacimuto, G.; Ozdemir, O.BACKGROUND: Febrile seizures (FS) represent the most common form of childhood seizures that occurs in 2-5 % of the children younger than 6 years. There have been many recent reports on the molecular genetic and pathogenesis of FC. It has been recognized that there is significant genetic component for susceptibility of FC with different reported mutation. FEB1, FEB2, FEB4, SCNA1, SCNA2, GABRG2 and IL-1 beta are related to with febrile convulsions (FCs). Interleukin 1 beta (IL-1 beta) is a cytokine that contributes to febrile inflammatory responses. There are conflicting results on increasing this cytokine in serum during FC. AIM: The determine the association between mutations of MEFV gene product pyrine and febrile seizures. PATIENTS AND METHODS: The study was carried out on 104 children that were diagnosed as FS and 96 healthy children. MEFV gene mutations were detected and analyzed with Pyro-Mark Q24. PCR was performed using the Pyro-Mark PCR Kit and pyrosequencing reaction was conducted on instrument instructions. RESULTS: M694V is the most common mutation in our patient group and we found a significant association between MEFV gene mutations and FSs. Of 104 patients, 68 were heterozygotes for any mutation and 10 patients were compound. 17.7% of control group were heterozygotes for any studied mutation. Statistical analyses showed that there was strongly significant statistical difference between results obtained from FS and control group (X = 46.20, p < 0.0001). CONCLUSIONS: MEFV gene mutations, especially M694V mutation, are positively associated with FSs.Öğe The Preventive Effects of Different Doses of Glucomannan on Experimental Aflatoxicosis in Japanese Quails(FACTA-FUNDACIO ARNCO CIENCIA TECNOLOGIA AVICOLAS, 2017) Yavuz, O.; Ozdemir, O.; Ortatatli, M.; Atalay, B.; Hatipoglu, F.; Terzi, F.This experimental study was performed to investigate whether there is a protective effect of different doses of Glucomannan using against aflatoxicosis in Japanese quail, and pathological changes and relative organ weights were compared. In the experiment, 60 one-day old male Japanese quails were used as divided into six different groups. Experimental groups were designated as Control(C), aflatoxin(A), glucomannan(GM), 2-fold dose of glucomannan(2GM), aflatoxin+glucomannan(A+GM) and aflatoxin+2-fold dose of glucomannan(A+2GM). While control group quails fed the standard ration as ad libitum, other groups were fed with the administrations additionally to standard diet respectively; 2mg/kg of aflatoxin to group A, 1g/kg of glucomannan to group GM, 2g/kg of glucomannan to group 2GM, 2mg/kg of aflatoxin and 1g/kg glucomannan to group A+GM, 2mg/kg of aflatoxin and 2g/kg glucomannan to group A+2GM. All quails were euthanized at day 21 of the study and organs, (liver, spleen, kidney, thymus and bursa of Fabricius) were removed, weighed and subjected to routine histopathological procedures. Although any important macroscopic changes were not observed in the C, GM and 2GM groups, significant pathological changes were found in the groups of A, A+GM and A+2GM. In the A+GM group, the partial reduction in the severity of microscopic lesions were seen in liver, bursa of Fabricius, thymus and spleen, however a significant reduction in severity of lesions was noticed in A+2GM group. As a result of the study, 2g/kg of glucomannan has been found pathologically to be more effective than 1g/kg glucomannan in terms of the protection against aflatoxicosis by giving orally.
