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    The morphometric properties of the lumbar spinal cord segments in horses
    (Medwell Journals, 2014) Selcuk M.L.; Bahar S.
    The aim of this study was to determine morphometric features of lumbar segments of the spinal cord of horses. Spinal cords of six horses from different breeds and sexes were used. Lumbar parts of the cords were dissected out from vertebral columns and segmentation was performed. Macroscopic measurements were done on the segments and then routine histological procedure was applied. Segments were sliced transversally at 3.8 mm thickness. Samples were cut at 10 ?m thickness and stained with May-Grunwald-Giemsa. Slides images were used by stereo investigator. Diameter measurements were carried out and area and volume calculations were performed by using Cavalieri principle. The weight, length and volume ratios of the lumbar parts to spinal cord were 14.7, 13 and 14%, respectively. A decrease in lengths of the segments from L1 to L6 was observed. In the microscopic analysis, L4 and L5 had the biggest transverse diameters however L6 had the largest vertical diameter. L5 had the largest values of cross-sectional and white matter area however L6 had the largest value of gray matter. Volumes of all segments except for L6 were similar to each other and the largest volume of gray matter and the largest volume of white matter were observed in L5 and L3, respectively. It was determined that the volume ratio of gray matter to the volumes of segments increased from L1 to L6 whereas volume of white matter was decreased in those segments. In conclusion, the data obtained will contribute to the morphology of related region and the further studies. © Medwell Journals, 2014.
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    Selective gray and white matter staining of the horse spinal cord
    (Veteriner Fakultesi Dergisi, 2012) Bolat D.; Bahar S.; Sur E.; Selcuk M.L.; Tipirdamaz S.
    The ratio of gray and white matter is an important clinical parameter in the diagnosis of diffuse and compressive diseases of the spinal cord. Although histological methods are used to determine this parameter, there are some difficulties encountered in histological studies related to tissue size. The aim of this study was to evaluate possible modifications to overcome these difficulties. In the study, nine tissue samples taken from the C6 segment of a female Shetland pony and selected by systematic random sampling were used. The dehydration process of the spinal cord of the horse was supported by applying a vacuum. Paraffin blocks were prepared and cut into 10 ?m sections to be stained separately with the different staining methods. Six different staining methods, including Modified May - Grunwald - Giemsa (MMGG), were compared and used to image entire slides. The stains, Hematoxylin & eosin (H&E), May-Grunwald-Giemsa (MGG), Masson's trichrome (MT), AgNORs, Kluver Barrera (KB) and MMGG, were evaluated macroscopically and microscopically by participants who were unaware of which staining methods had been used. The staining methods were scored from worst (1) to best (5) using a Likert scale. Vacuum application was found to reduce the difficulties related to inadequate tissue dehydration. MMGG was selected as the best staining method in differentiating gray and white matter in the spinal cord of the horse.