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Öğe Bi?r Köpekte Multiple Pri?mer Tümör Olgusu(Selçuk Üniversitesi, 1993 Ocak) Erer, Hüdaverdi; Kıran, Metin Münir; Tekeli, TevfikIn this case multiple primary neoplasia was described. Mixed tumor and papillary adenocarsinoma of mammary gland, intrahepatic cystic cholangiocellular adenoma, vaginal fibramatosis and nodular hyperplasia of spleen were detected in a 10 years old setter bitch This case was the first report on multiple primary tumors in different organs of dogs in Turkey.Öğe Bir inekte karşılaşılan yalancı meme başı fistülünün real-time ultrasonografi ile ayırıcı tanısı(1996) Tekeli, Tevfik; Erdem, Hüseyin; Uçar, MehmetBu olguda 2.5 yaşında ye bir doğum yapmış Holstein ırkı bir ineğin sol ön meme başının posteriorunun üst 2/3'ünde karşılaşılan meme başı fistülünün real-time ultrasonografi ile ayırıcı tanısı üzerine bilgi verilmiştir.Öğe Determination of milk and plasma progesterone concentrations for early diagnosing pregnancy in cows(Selçuk Üniversitesi, 1987) Tekeli, Tevfik; Sezer, A. Nuri; Alaçam, ErolSummary: The aim of this study was to investigate the pregnancy diagnosis rates by blood and milk serum progesterone assays at the first and second estrous periods after the natural servings in cows and heifers. Fourty Swedish red cows and 10 heifers, between 2- ll years old, were used as materials. Blood and milk samples w ere collecteld from cows in addi tion to blood samples taken from heifers on the days of 21, 38 and 45 days after serving. Blood and milk samples were centrifugated for 20 minutes at the 2000 and 5000 rpm respectively and were kept in at- 20 until the determination of progesterone by radioimmunoassay method.Öğe Effect of PRID applications and cloprostenol injections on various fertility parameters in dairy cows [Sütçü Ineklerde PRID ve Cloprostenol Uygulamalarinin Çeşitli Fertilite Parametrelerine Etkisi](1996) Alaçam, Erol; Tekeli, Tevfik; Dinç, D. Ali; Güler, Mehmet; Işık, KürşatThis study was designed to determine the fertility parameters in dairy cows after timed insemination and simultaneous treatment with PRID or cloprostenol. Treatments commenced days 40 and 52 post partum. A total of 36 cows was randomly assigned to three groups. Cows in the each group were treated either with progesterone releasing intravaginal device (PRID) for 12 days, or two injections of 500 mcg cloprostenol at interval of 11 days, or left as controls. Cows in treatment groups were inseminated 60. and 72. hours after the end of treatments and in spontane estrous of controls. The events of first and following two inseminations were discussed. In the groups of PRID, cloprostenol and controls: pregnancy rates were 41.6, 66.6 and 50.0%, durations of calving-conception 91.4±8.4, 68.1±5.3 and 93.5±10.4 days, and inseminations per conception were 2.2, 1.2 and 1.6 respectively. Looking the more advantageous results of cloprostenol group, the injections of prostaglandin F2 alpha analoques 11 days apart is better of choise procedure in the planning of fertility programmes for dairy herds.Öğe Effect of Seasonal Conditions on Oestrus Occurrence and Postpartum Period in Anatolian Water Buffaloes(M H Schaper Gmbh CO Kg, 2002) Aksoy, Melih; Kaya, Abdullah; Uçar, Mehmet; Lehimcioğlu, Necdet Cankat; Tekeli, TevfikOestrus and calving records of Anatolian buffaloes were analyzed to investigate influence of climatic conditions on oestrus occurrence and postpartum period. Oestrus records showed a seasonal pattern concentrated between July and September which is the warmest period of the year. Likewise, more than half (52.7%) of the total calvings occurred between May and August. Month of calving significantly influenced the length of calving to first oestrus interval and open period (P < 0.01). The average length of open period was significantly shorter in autumn and summer calvers compared to those calved in spring and winter (P < 0.05) during long days. The humidity rate was negatively correlated with both calving to first oestrus interval and open period (P < 0.05). In conclusion, exposure to high environmental temperatures around 20 degreesC exerted no suppressive influence on ovarian activity in Anatolian buffaloes. Although humidity rate (P < 0.05) and ambient temperature (P < 0.01) was found to be correlated with the duration of postpartum period, day length might be the main factor regulating reproductive biorhythm and postpartum events in Anatolian buffalo cows.Öğe Effect of site of insemination on bovine fertility(1997) Ataman, Mehmet B.; Taşal, İbrahim; Aksoy, Melih; Karaca, Fikret; Kaya, Abdullah; Tekeli, TevfikBu çalışmada, suni tohumlama yapılan inek ve düvelerde sperma bırakılma yerinin gebelik oranları üzerine etkisi araştırıldı. Çalışmada 82 baş inek ve 18 baş düve olmak üzere toplam 100 baş hayvan cervical, corpus uteri, Graaf folliküiünün iokalizasyonuna göre ipsilateral ve kontralateral cornu tohumlama bölgesi olmak üzere 4 eşit gruba ayrılarak tohumlandılar. Gebelik oranları gruplarda sırasıyla %88, %72, %84 ve %72 olarak tespit edildi. Sonuç olarak, spermanın corpus uteri veya ipsilateral cornuya depo edilmesinin gebelik oranını arttırdığı, cervical yolla tohumlamanın herhangi bir avantajı olmadığı kanısına varıldı.Öğe Estimation of early pregnancy by electrical resistance values of vaginal mucosa in cows and heifers(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2005) Taşal, İbrahim; Ataman, Mehmet Bozkurt; Aksoy, Melih; Kaya, Abdullah; Karaca, F; Tekeli, TevfikThe aim of this study was to investigate the availability of vaginal resistance values to estimate early pregnancy in heifers and cows on days 18-21 post-insemination. Seven Brown Swiss cows and ten heifers without any palpable reproductive disorders were used. Cloprostenol, a synthetic analogue of PGF(2 alpha), was administered intramuscularly twice (500 mu g) 11-days apart. After second cloprostenol administration, animals were observed for the overt signs oestrus and, inseminated twice with frozen semen after confirmation of oestrus by rectal palpation. Electrical conductivity of vaginal mucosa was measured through ventral wall of vagina adjacent to caudal end of cervix uteri by a handy-held instrument. Vaginal conductance of animals was measured twice, with a 12 h interval, on days 0 (day of oestrus), 3, 6, 10, 11 19 20 and 21 in cows, and on days 0 (day of oestrus), 3, 6, 10, 13, 18, 19 and 20 in heifers. To estimate plasma progesterone levels, jugular blood samples (10 mL) were also collected on days 19, 20 and 21 for cows, and 18, 19 and 20 for heifers. Pregnant animals were detected by rectal palpation 60 days after insemination. In pregnant cows, electrical resistance of vaginal mucosa measured between days 19-21 was significantly higher than non-pregnant animals, while vaginal resistance values determined on the day of insemination, and on days 3, 6, 10 and 13 post-insemination were similar in both groups. Likewise, vaginal impedance readings in pregnant heifers determined between days 18-20 were significantly higher than those non-pregnant animals. Vaginal resistance measurements in cows (between days 19-21) and heifers (between days 18-20) were significantly correlated with plasma progesterone concentrations both in pregnant and non-pregnant groups. It was concluded that vaginal resistance values might be used for the diagnosis of early pregnancy as a contributor or predictor method in cows and heifers.Öğe Estrus and Pregnancy Rates of Konya Merino Ewes Treated with a Double Injection of Cloprostenol at Different Intervals(ARCHIV FUR TIERZUCHT, 1997) Tekeli, Tevfik; Aksoy, Melih; Semacan, A.; Karaca, F.; Ayar, A.The effect of a double injection of cloprostenol given at different intervals on estrus and pregnancy rates after artificial insemination were evaluated in cycling Konya Merino ewes. Two injections of 125 mu g of cloprostenol were given to the ewes in group I (n=30), II (n=30) and III (n=31) at an interval of 8, 11 or 14 days, respectively. Ewes in group IV (n=28) served as control and received a double injection of saline at an interval of 8 days. Estrus ewes were detected by teaser rams during five days, 17 days in group IV, following the second treatment and intracervically inseminated by fresh semen. Estrus and pregnancy rates after first insemination in group I II, III and IV were 86.7, 100.0, 83.9 and 89.3% and 50.0, 60.0, 80.6 and 75.0%, respectively. It was concluded that an interval of 14 days may provide better pregnancy rates compared to those 8 and II days intervals in artificially inseminated ewes.Öğe Fare Ovumlarının In Vitro Fertilizasyonu Üzeri?nde Çalışmalar(Selçuk Üniversitesi, 1990 Ocak) Tekeli, Tevfik; Güler, Mehmet; Aksoy, MelihThe aim of this study was to test the recommended techniques of in vitro fertilization in mice as an experimental animal. Adult female mice of Swiss albino strain were superovulated by intraperitonal Injection of PMSG and HCG 48 hours apart. They were killed 16 hours after injection of HCG and their eggs were recovered under warm oil in a plastic dish containing 0.4 ml of incubation medium. Sperms recovered from cauda epididymis of adult males of the same strain were suspended in a medium and incubated at 37 deg C under % 5 CO? in air for 1.5-2 hours before they were used for insemination. The incubation medium was THY me- dium containing glucose, sodium pyruvate, bovine albumin and antibiotics. At the time of insemination, a drop of sperm suspension (10-20 ?l) was added to the medium containing egg clot. Eggs and sperms were incubated in incubation medium for 6 hours. In the first group, fertilized eggs were left into the same medium. In the second group they were transferred to the development medium (Modified Whitten's) without sodium lactate and incubated until they were examined under stereomicroscope. As a conclusion, in this study fertilized eggs and 2-cell stage embryos were obtained but further stage of embryos (morulae, blastocyst) couldn't be reached.Öğe Geç emriyonal ve erken fötal dönemde Konya merinosu koyunlarda gebelik tanısı ve fötal sayım amacıyla transabdominal ultrasonografik muayene tekniğinin etkinliğinin değerlendirilmesi(2008) Erdem, Hüseyin; Sarıbay, M. Kemal; Tekeli, TevfikSunulan çalışmada Konya Merinosu ırkı koyunlarda gebelik tanısı ve fötal sayım amacıyla geç embriyonal ve erken fötal dönemde sağ (R) ve sol (L) inguinal bölgeden yapılan transabdominal ultrasonografik muayenenin (TAUM) etkinliğinin belirlenmesi amaçlandı. Bu amaçla aşım sonrası 34. günde transrektal ultrasonografik muayene (TRUM) ile 45 baş tek ve 39 baş ikiz embriyo taşıyan gebe koyun belirlendi. Toplam 84 baş gebe koyun 34 ve 50. günlerde, RTAUM ve LTAUM yoldan farklı saatlerde muayene edilerek, bulgular TRUM ve doğum kayıtları ile karşılaştırıldı. TRUM ve doğum kayıtlarına göre 34. günde tekli gebelik RTAUM ve LTAUM olarak sırasıyla % 62 (28/45) ve % 64 (29/45), 50. günde de sırasıyla % 88 (40/45) ve % 91 (41/45) oranında doğru olarak belirlendi. İkiz gebelikler ise RTAUM ve LTAUM olarak 34. günde sırasıyla % 33 (13/39) ve % 28 (11/39) oranında belirlenebilirken; 50. günde sırasıyla % 53 (21/39) ve % 46 (18/39) oranında doğru olarak belirlendi. Bulgulara göre gerek muayene günleri ve gerekse muayene bölgeleri arasında istatistiki farklılık görülmedi. Sonuç olarak ultrasonografik muayenede ikiz gebeliklerin ilk 50 günde RTAUM ve/veya LTAUM olarak yüksek oranda belirlenemediği; fötal sayımın önemli olmadığı durumlarda ise muayenelerin 34. günden itibaren transabdominal (TA) yolla yapılabileceği kanısına varıldı.Öğe GnRH Response Test and Libido Scores in Normal and Low-Quality Sperm Producing Rams(BLACKWELL WISSENSCHAFTS-VERLAG GMBH, 1993) Aksoy, Melih; Tekeli, Tevfik; Çoyan, K.; Güven, B.; Özar, S.; Alan, M.; Ayar, AliTesticular tissues are responsible for the androgen synthesis as well as the spermatogenesis. Production rate of androgens is affected by many factors. In addition to the hypothalamo- pituitary control, possibly a local substance, which is released by damaged seminiferous tubules, also plays an important role in androgen production of the Leydig cells (Aoki & Fawcett, 1978). Testosterone synthesis can be stimulated by the injection of exogenous gonadotropins and releasing hormones (Hocherau et al., 1990; Post et al., 1987 a,b). Lunstra & Schanbacher (1988) showed that cryptorchid rams had lower basal testosterone levels and a lesser ability to secrete testosterone in response to exogenous LH. Byers & Glover (1984) investigated the effect of scrotal insulation on hormone levels and pointed out that the mean and basal testosterone levels were significantly reduced after 14 days following scrotal insulation. Veeramachaneni et al. (1986) suggested that azoospermic bulls have lower baseline testosterone levels and testosterone response rates to GnRH injections than nor- mozoospermic bulls. GnRH induced testosterone levels in stallions are used as a diagnostic tool to differentiate cryptorchid and castrated animals (Nachrainer, 1986). Post et al. (1987b) reported that GnRH induced testosterone levels might give a valuable information on the selection of the bulls which are adequate to reproductive potential. The present study was designed to evaluate the GnRH response test results of low quality sperm producing and normozoospermic rams comparatively. Libido tests were also performed to assess the libido level of rams with normal or low sperm output.Öğe Holstein Irkı Düvelerde Tohumlamayı İzleyen 12. Günde GnRH Uygulamalarının Fertilete Üzerine Etkisi(2002) Erdem, Hüseyin; Tekeli, Tevfik; Yenice, MustafaThe effect of GnRH analogue on the pregnancy rate was studied in heifers in Konya province. A total of 40 Holstein heifers aging between 15-16 months was included in the study. The heifers in oestrus detected by rectal palpation were divided into 2 groups randomly. First group received 10 \mug buserelin (GnRH) intramuscularly on the 12th day after the first insemination, and second group had 2.5 ml placebo intramuscularly. Pregnancy rates in the GnRH and placebo groups after the first insemination were 70 and 45 % respectively (p<0.001). All of the heifers were pregnant on the 4th insemination and per pregnancy rates were 1.35 and 1.85 insemination in GnRH and placebo groups, respectively. Blood was taken in order to determine the plasma progesterone levels on the 0 (insemination day), 4, 8, 12, 14, 16, 18, 20 and 22 days. There were no statiscally significant differences on the progesterone levels between the GnRH and placebo groups in the pregnant heifers. On the other hand, GnRH group had statiscally lower (p<0.05) progesterone levels than placebo group in the nonpregnant heifers on the 14th day. It was concluded that embryonic deaths resulting from luteal insufficiencies can be prevented with application of GnRH analogue on the 12th day of insemination, but sperm quality, anovulation and delayed ovulation as ovulatory defect as a cause of wrong insemination time might effect the results, therefore in this type of studies, endochrinologic examinations must also be carried out.Öğe Holstein ırkı ithal gebe düvelerden oluşan sürünün doğum sonrası dölverimi performansının değerlendirilmesi(1998) Tekeli, Tevfik; Erdem, Hüseyin; Uçar, Mehmet; Aksoy, Melih; Yenice, MustafaSunulan çalışmada, Konya'da bulunan özel bir sütçü işletmeye ait, tamamı Türkiye'ye ithal edilen 270 baş damızlık gebe düveden oluşan bir sürünün doğumlarından sonra dölverimi performansı değerlendirildi. İthalat sonrası 10. ayda düvelerin yaptığı doğum şekli, suni tohumlamalarda elde edilen gebelik oranı ve infertilitenin etiyolojisi rektal palpasyon ve ultrasonografık muayenelerle belirlendi. Sürüde ithalat sonrası normal doğum, abortus, güç doğum, ölü doğumların oranları sırasıyla % 81.3, 6.0, 10.1, 2.6 olarak bulundu. Sürünün ithalat sonrası 10. ayda yapılan muayenesinde gebelik oranı % 57.8 olarak tespit edildi. Gebe olmadığı belirlenen ineklerin % 15.9'unda uterus enfeksiyonu, % 8.9'unda ovaryum kisti ve % 17.4'ünde de dölverimi ile ilgili diğer sorunlar belirlendi. Birinci, ikinci ve üçüncü tohumlamada gebelik oranları sırasıyla % 25.9, 50 ve 57 olarak tespit edildi. Gebe kalan ineklerde gebelik başına yapılan tohumlama sayısı 1.67 olarak bulundu. İlk üç tohumlamada gebe kalan bütün inekler ile birinci, ikinci ve üçüncü tohumlamalarda gebe kalan ineklerde doğum-ilk tohumlama aralığı sırasıyla 76.41.9, 79.63.1, 74.42.7, 71.74.6 gün olarak belirlendi. İkinci ve üçüncü tohumlamalarda gebe kalan ineklerde gözlenen toplam 103 östrüs siklusunun % 34.0'nün normal sayılan 18-24 günlük sınırlar içerisinde, % 66.0'nın bu sınırların dışında olduğu tespit edildi. Sonuç olarak, ithal edilen gebe düvelerde bakım ve idari sorunların yanı sıra Türkiye koşullarına adaptasyon güçlüğüne bağlı olarak dölverimi ile ilgili büyük sorunlarla karşılaşıldığı; bu nedenle düve veya inek ithalatı yerine en azından adaptasyon sorunlarının giderilebilmesi amacıyla donmuş embriyo ithalatının daha başarılı olacağı kanısına varıldı.Öğe I?n Vi?tro Döllenmi?ş Fare Ovumlarının Geli?şmesi? Üzeri?nde EDTA'nın Etki?si?(Selçuk Üniversitesi, 1992 Ocak) Tekeli, TevfikThe present study was undertaken to wheth-er EDTA exerts beneficial effect on the development of mouse embryos derived from eggs fertilized in vitro. Superovulated eggs were collected from ddY females and were inseminated with epididymal sperm obtained from ddY males. At 6 hours after insemination the fertilized eggs were transferred to the Whitten's medium with or without EDTA and then cultured for 24 to 120 hours. The development of embryos beyond the 2- cell stage at 48 hours was significantly enhanced by the presence of 10 mM EDTA. Fifty seven per cent of embryos cultured for 96 hours in the medium containing EDTA developed morulae stage. Only 13% of embryos cultured for 96 hours in the medium without EDTA developed into morulae stage. On the other hand, 27% of embryos cultured in the medium with EDTA developed into blastocyst stage. As a conclusion, the development of in vitro fertilized mouse embryos beyond the 2 cell stages in Whitten's medium was enhanced by the presence of EDTA.Öğe I?nek ve Mandalarda Subkli?ni?k Masti?ti?sleri?n Tanısı, Etkenleri?n I?zolasyonu ve Bunlara Karşı Etkili? Anti?bi?yoti?kleri?n Beli?rlenmesi?(Selçuk Üniversitesi, 1989 Ocak) Alaçam, Erol; Tekeli, Tevfik; Erganiş, Osman; İzgi, A. NedimIn this study, diagnosis, isolation of microbiological agents and antibiotic susceptibility results were comparatively determined on subclinically mastitic cows and buffalos in the same farm at differents seasons. The cell counts of the milk samples collected according to the scores of CMT in cows and buffalos were 1.7 * 0 = 320 and 1.34 .000 ne 290.000 respectively The incidence of subclinical mastitis cases were between 6.73-17.25 % in cows and 4.68-116125 % in buffalos during the period of study. On the other hand high incidence was observed during summer months. The staphylococcus spp were on the top levels as a 26.19 % in cows and 37.50% in buffalos. Following this, Corynebacterium spp rates in cows and buffalos were 30.95 % and 8.33% respectively. In additon to these, Streptococcus spp, E. coli, K. pneumonia, E. aeruginosa, B. cereus, Anaerobic gram (+) bacillus, Aspergillus spp, Penicillum spp, Candida spp were also isolated in low rates. Antibiotic susceptibility test results were screened on the table. The differencies statistically was not important in the subclinical mastitis incidence rates, microorganisms and effective antibiotics between the cows and buffalos.Öğe I?neklerde Corpus Luteumun Morfolojik Özellikleri ve Endokrinolojik Aktivitesi Arasındaki İlişkinin Araştırılması(2000) Kaya, Abdullah; Erdem, Hüseyin; Aksoy, Melih; Tekeli, TevfikBu çalışmada luteal evredeki ineklerde corpus luteum (CL)' un morfolojik özellikleri ile endokrinolojik aktivitesi arasındaki ilişki belirlendi. Araştırma kesim amacıyla mezbahaya getirilen toplam 135 baş Holstein ırkı inek üzerinde gerçekleştirildi. İnekler önce rastlansal olarak numaralandırıldı ve kesimden önce kan örnekleri toplandı. Kesim sonrasında her ineğin ovaryum ve uterusları toplanarak laboratuvara getirildi. Gebe olan, inaktif ovaryumlu veya seksüel siklusun diöstrüs evresi dışında bulunan inekler çıkartıldıktan sonra gözlenebilir aktif CL' a sahip ve plazma progesteron düzeyi l ng/ml' den yüksek 60 baş inek araştırma grubuna alındı ve bulgular değerlendirildi.Corpus luteum çapı. ağırlığı ve plazma progesteron düzeyi arasında önemli pozitif korelasyon tespit edildi (pO.Ol). Çalışmada incelenen toplam 60 baş inekte kavile formasyonu gözlenen CL' ların görülme oranı % 25 olarak belirlendi. Kaviteli ve kavitesiz CL' a sahip inekler arasında plazma progesteron düzeyi. CL çapı ve CL ağırlığı arasında farklılık bulunamazken kaviteli CL' larda endokrin aktivite (plasma progesteron düzeyi (ng/ml) / CL ağırlığı (gr) düzeyi önemli oranda düşük bulundu (p0.05). Araştırmada incelenen 3 farklı CL tipi arasında endokrin aktivite yönünden farklılık bulunamadı.Sonuç olarak, CL' un kavite formasyonunun ultrasonografik olarak incelenmesinin yanısıra ağırlığı ve çapının da endokrin aktivitesi hakkında değerli bilgiler verebileceği kanısına varıldı.Öğe I?neklerde Ovaryum Palpasyonu Yöntemiyle Seksüel Siklus Dönemlerinin Belirlenebilirliğinin Araştırılması(2000) Kaya, Abdullah; Erdem, Hüseyin; Çoyan, Kenan; Tekeli, TevfikBu araştırmanın amacı ineklerde östrüs siklusu evrelerinin belirlenmesinde rektal yolla ovaryum palpasyonunun etkinliği araştırıldı. Araştırmada toplam 88 baş siklik Holstein ırkı inek kullanıldı. İnekler, seksüel siklus evrelerinin belirlenmesi amacıyla önce rektal palpasyon ile muayene edildiler. Buna ilave olarak plazma progesteron düzeylerini ölçmek amacıyla kesimden önce ineklerin kan örneklen alındı, kesimden sonra ise uterus ve ovaryumlar toplandı. İnekler.'ovaryum ve corpus luteumların gözlenmesi ve plazma progesteron düzeyleri esas alınarak folliküler, ovulasyon sonrası ve luteal dönem olmak üzere 3 evreye ayrıldı. Buna göre ineklerde folliküler. ovulasyon sonrası ve luteal evrelere dağılım oranları sırasıyla % 19.3, % 12.5 ve % 68.2 olarak bulundu. Foliiküler. ovulasyon sonrası ve luteal dönemde bulunan inekler ovaryum palpasyonu yöntemiyle sırasıyla % 70.6, % 45.4 ve % 83.4 oranında doğru, olarak belirlendi. Ortalama plazma progesteron konsantrasyonu folliküler, ovulasyon sonrası ve luteal dönemde sırasıyla , 0.22 0.07, 0.29 0.10 ve 2.64 0.19 ng / mi olarak bulundu. Bu sonuçlar ovaryum palpasyonu ile ineklerin bulundukları seksüel siklus evrelerini belirlemede bazı zorlukların bulunduğunu ve çeşitli oranlarda hatalı tanı yapılabileceğini göstermiştir. Bu nedenle yalnızca rektal palpasyon yöntemiyle ineklerin siklus dönemlerinin tanınmasının her zaman mümkün olmadığı sonucuna varılmıştır.Öğe I?neklerde Subkli?ni?k Masti?ti?sleri?n Bi?yoki?myasal ve Mi?krobi?yoloji?k Yönden I?ncelenmesi?(Selçuk Üniversitesi, 1989 Ocak) Nizamlıoğlu, Mehmet; Tekeli, Tevfik; Erganiş, Osman; Başpınar, NuriThe aim of this study was to investigate some biochemical and microbiological values for the early diagnosis of subclinical mastitis in healthy and mastitic cows. In the present study, a total of 30 Brown-Swiss cows, in 5-7 years old, supplied by Konya Animal Research Centre, were used as materials. Blood samples were taken from the animals at the same time as the milk. All the biochemical and microbiological determinations were performed on the same day. ALP, albumin, Na and K values were determined in the blood serum samples. The counting of the PMN, LDH, ALP, Na and K values were measured in the milk samples. In the subclinical mastitic cows, milk LDH, ALP, Na ve K values were found statistically significant. Furthermore, the counting of the PMN increased significantly. Blood serum sample values were not found statistically significant. As a result, it was useful to determine of the LDH, ALP, Na, K and the counting of the PMN in milk for the early diagnosis subclinic mastitis. Other tests, blood serum albumin, Na and K values were not found mainly important for the early diagnosis of the subclinic mastitis.Öğe I?neklerde Subkli?ni?k Masti?ti?sleri?n Kuru Dönemde Peni?si?li?n - Streptomi?si?n Kombi?nasyonuyla Sağıtımı Üzeri?nde Araştırmalar(Selçuk Üniversitesi, 1985 Ocak) Tekeli, Tevfik; Baysal, Tülay; Gökçay, YılmazIn this study, the effects of penicilin - streptomycin on subclinical mastitis in dairy cattle was investigated during the dry period. The milk samples of 39 cows that were at the end of the lactation period were collected aseptically. They were examined bacteriologically whether they were infected or not. Pathogenic bacteriae were isolated from 46 milk samples. Then, 46 infected quarters were treated by penicillin - streptomycin combatination. To determine the effectiveness of the treatment, milk samples were collected from the mammary quarters after calving and were examined bacteriologically. After calving 84.78% of these infected quarters were found to be free from infection. Streptococci and E. Coli were eliminated by 100% and staphylococci and C. bovis infections were eliminated 85% during the same period.Öğe In vitro culture of in vivo Saanen goat embryos by vitrification(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2016) Kose, Ayse Merve; Tekeli, TevfikThe purpose of this study was to investigate the survival rate of high-quality embryos (n: 101) derived in vivo from Saanen goats (n: 15) in culture following their vitrified freezing and thawing during the breeding season. The in vitro postthaw survival rates of 101 vitrified embryos at 24, 48, and 72 h were 59.4%, 33.6%, and 25.7%, respectively. According to the developmental stage of the embryos, the survival rates were 51.3%, 20.5%, and 15.4% in the morula, and 64.5%, 41.9%, and 32.3% in the blastocyst, respectively. The survival rates of the blastocysts and morulae at 48 and 72 h were significantly different (P < 0.05). While the survival rates of the embryos of Grade 1 quality at 24, 48, and 72 h were 78.6%, 46.4%, and 32.1%, Grade 2 quality survival rates were 35.5%, 17.8%, and 17.8%, respectively. The survival rates of Grades 1 and 2 embryos at 24 h (P < 0.001) and 48 h (P < 0.05) were significantly different. As a result, it was concluded that the embryos to be frozen should be selected according to their development stage and quality, and preferably should be of Grade 1 quality in the blastocyst stage for the cryopreservation of in vivo-derived goat embryos by vitrification.
