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Öğe Antioxidative effects of cysteamine, hyaluronan and fetuin on post-thaw semen quality, DNA integrity and oxidative stress parameters in the Brown Swiss bull(WILEY, 2015) Sariozkan, S.; Tuncer, P. B.; Buyukleblebici, S.; Bucak, M. N.; Canturk, F.; Eken, A.The aim of this study was to compare the effectiveness of antioxidants including cysteamine (2.5, 7.5mm), hyaluronan (0.25, 1mgml(-1)) and fetuin (5, 10mgml(-1)) in the freezing of Brown Swiss bull semen. The best percentages of CASA motilities were achieved with 10mgml(-1) of fetuin and 2.5mm of cysteamine. For sperm morphology, 10mgml(-1) of fetuin and 2.5mm of cysteamine had better protective effects (P<0.001). The results of hypo-osmotic swelling test showed that the percentage values of membrane integrity in all the groups, excluding that supplemented with 5mgml(-1) of fetuin, were higher than those of the control group (P<0.001). Results obtained for the DNA damage of sperm cells demonstrated that 0.25mgml(-1) of hyaluronan, and 2.5 and 7.5mm of cysteamine led to lower rates of spermatozoa with damaged DNA, compared with the control group (P<0.001). The maintenance of superoxide dismutase and glutathione peroxidase antioxidant activities following freeze-thawing with 2.5 and 7.5mm of cysteamine and 10mgml(-1) of fetuin was demonstrated to be at a higher level in comparison with the control group (P<0.001). Malondialdehyde formation was found to be lower in the groups supplemented with 0.25mgml(-1) of hyaluronan and 7.5mm of cysteamine after the freeze-thawing process (P<0.001).Öğe Effects of curcumin and dithioerythritol on frozen-thawed bovine semen(WILEY, 2012) Bucak, M. N.; Başpınar, Nuri; Tuncer, P. B.; Coyan, K.; Sarıozkan, S.; Akalın, Pınar Peker; Büyükleblebici, S.The aim of this study was to determine the effects of curcumin and dithioerythritol added into bull semen extender on sperm parameters, lipid peroxidation, total glutathione and antioxidant potential levels of bull spermatozoa following the freeze/thawing process. Twenty-seven ejaculates obtained from three bulls were included in the study. Each ejaculate that was splitted into five equal groups and diluted in a Tris-based extender containing curcumin (0.5 and 2 mm), dithioerythritol (0.5 and 2 mm) and no additive (control) was cooled to 5 degrees C and frozen in 0.25-ml French straws. The extender supplemented with 0.5 mm dose of curcumin led to lower percentage of total abnormality (20.40 +/- 2.36%) when compared to the control (30.60 +/- 1.47%, P < 0.05). Curcumin and dithioerythritol at 0.5 mm provided a greater protective effect in the membrane functional integrity (54.40 +/- 2.09% and 50.00 +/- 2.68%), in comparison with control (37.20 +/- 1.77%, P < 0.001). Supplementation with antioxidants did not significantly affect the lipid peroxidation and antioxidant potential levels, while the maintenance of total glutathione levels in curcumin 0.5 mm was demonstrated to be higher than that of control, following the freeze/thawing (P < 0.05). Supplementation with these antioxidants prior to the cryopreservation process may be recommended to facilitate the enhancement of sperm cryopreservation techniques.Öğe Effects of some cryoprotectants and antioxidants on frozen/thawed bull sperm motility and motion characteristics(WILEY-BLACKWELL, 2011) Tuncer, P. B.; Bucak, M. Numan; Buyukleblebici, S.; Kuzuguden, F.; Sariozkan, S.; Tasdemir, U.; Kinet, H.[Abstract not Available]
