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    Cell death induced by eluates from hypoallergenic denture base acrylic resins in NIH-3T3 fibroblast cells
    (ELSEVIER TAIWAN, 2014) Kansu, Gulay; Kalyoncuoglu, Tugba; Uyar, Pembegul; Uzun, Esra
    Background/purpose: The use of a hypoallergenic denture base material for dental patients is susceptible to trigger allergic reactions. The aim of this study was to compare the cytotoxicity at the cellular level of hypoallergenic denture base materials and polymethyl methacrylate denture base materials polymerized in different ways. Materials and methods: Comparisons were done with the MTT (3-4,5-dimethylthiazol-2-yl)-2,5-dephenyltetrazolium bromide) results of eluates, which were derived from all test materials by immersing for 24 and 48 hours in various ratios of the test materials' weights to the volume of Dulbecco's modified Eagle's medium without supplements (0.2, 0.4, and 0.8 g/mL) and incubating NIH-3T3 cells for 24 and 48 hours (N = 9). Then the apoptotic effects of the materials were detected, using the TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay. Results: The 24-hour eluates of all materials exhibited strong cytotoxicity, and cytotoxicity increased with time but the difference was not statistically significant. In terms of cell death rates, the materials were ranked as follows: QC 20, Puran HC, Acron MC, Promysan Star. As a result of the apoptotic index of the materials, the ratio of necrotic cells was found from highest to lowest: QC 20, Puran HC, Acron MC, and Promysan Star. Conclusion: It was determined that the test materials have cytotoxic effects on fibroblasts, and polymethyl methacrylate-based test materials have a more necrotic effect on cells; however, other test materials induced apoptosis at a higher range. Copyright (C) 2013, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. All rights reserved.
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    Fluorescent labelling of DNA on superparamagnetic nanoparticles by a perylene bisimide derivative for cell imaging
    (ELSEVIER SCIENCE BV, 2015) Maltas, Esra; Malkondu, Sait; Uyar, Pembegul; Ozmen, Mustafa
    N,N'-Bis[tris-(2-aminoethyl) amine]-3,4,9,10-perylenetetracarboxylic diimide (PBI-TRIS), nonfluorescent dye was used to fluorescent labelling of DNA. For this aim, (3-aminopropyl) triethoxysilane (APTS) modified superparamagnetic iron oxide nanopartides (SPIONs) were synthesized to provide a suitable surface for binding of DNA. Amine functionalized nanoparticles showed a high immobilization capacity (82.70%) at 25 mg of nanoparticle concentration for Calf thymus DNA. Binding capacity of PBI-TRIS to DNA-SPION was also found as 1.93 mu M on 25 mg of nanoparticles by using UV-vis spectroscopy. Binding of PBI-TRIS to DNA onto nanoparticles was also characterized by scanning electron microscopy and infrared spectroscopy. The confocal images of PBI-TRIS labelled DNA-SPION and breast cells were taken at 488 and 561.7 nm of excitation wavelengths. Cell image was also compared with a commercial dye, DAPI at 403.7 nm of excitation wavelength. Results showed that PBI-TRIS can be used for cell staining. (C) 2014 Elsevier B.V. All rights reserved.