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Serological investigation of bluetongue virus infection by serum neutralization test and ELISA in sheep and goats
(NATL VETERINARY RESEARCH INST, 2006) Bulut, Oya; Yavru, Sibel; Yapkic, Orhan; Simsek, Atilla; Kale, Mehmet; Avci, Oguzhan
Altogether 562 sheep and goat blood serum samples were collected from livestock located in Konya, Burdur, and their environs in Turkey. The samples were tested for bluetongue virus antibodies by ELISA and serum neutralization test (SNT). Fifty-four (17.1%) out of 315 sheep serum samples from Konya and 1 (1.5%) out of 66 sheep serum samples from Burdur were detected as seropositive. Seventy-three (60%) out of 121 goat serum samples from Konya and 36 (60%) out of 60 goat serum sample from Burdur were found positive by ELISA. Fifty-three (16.8%) out of 315 sheep serum samples from Konya and none of 66 sheep serum samples from Burdur were detected to be seropositive. Sixty-five (53.7%) out of 121 goat serum samples from Konya and 32 (53.3%) out of 60 goat serum samples from Burdur were found as positive by SNT. The results demonstrate that ELISA is more sensitive than SNT.