Yazar "Yildirim, Sibel" seçeneğine göre listele
Listeleniyor 1 - 14 / 14
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Characterization of dental pulp defect and repair in a canine model(MOSHER & LINDER, INC, 2011) Yildirim, Sibel; Can, Alp; Arican, Mustafa; Embree, Mildred C.; Mao, Jeremy J.Purpose: To explore a relationship between the size of pulp chamber perforation and reparative dentin formation in a canine model. Methods: Pulp defects were created in the pulp chambers of maxillary and mandibular premolars (N=64) in 17 healthy mongrel dogs in three different sizes (diameter/depth: 1/1, 2/1, and 2/2 mm(3)) with sterile round burs under general anesthesia. The perforations were immediately capped with hard-setting calcium hydroxide (CH) in the control group or sealed with Teflon membrane (TM) in the experimental group, followed by restoration with reinforced zinc oxide eugenol cement in vivo. Seven and 30 days after pulp chamber perforation and restoration all treated and control premolars were extracted and prepared for histomorphometric and statistical analyses. Results: Reparative dentin formation was more pronounced for defect sizes up to 2/1 mm(3) when treated with CH, and completely bridged the surgically created dentin defects only after 30 days. However, reparative dentin upon CH treatment failed to completely bridge pulp chamber exposure for 2/2 defects. By contrast, TM treatment only yielded mild reparative dentin bridging for defects up to 1/1, but not for either 2/1 or 2/2 defects at 30 days. Inflammatory responses of the exposed dental pulp tissue were more robust with the TM group than with the CH group. Thus, dental pulp tissue possesses a capacity for spontaneous repair by the formation of reparative dentin in this preclinical model, but only up to a defect size of similar to 2 mm in diameter and 1 mm in depth. All observations are based on 30 days post-treatment in the canine model. These findings may serve as baseline for regenerative endodontic studies (Am J Dent 2011;24:331-335).Öğe Öğe Öğe Öğe Öğe Öğe Öğe Öğe Effect of avulsion storage media on periodontal ligament fibroblast differentiation(WILEY, 2017) Bag, Irem; Yildirim, SibelBackground/AimAn avulsed tooth must be stored in a solution which maintains periodontal ligament (PDL) cell viability. The aim of this study was to compare the effects of Hank's balanced salt solution (HBSS) and milk on the differentiation of PDL fibroblasts. Materials and methodsEighteen extracted third molars, (n=3 for each group), were immersed in HBSS, milk, and Dulbecco's modified Eagle medium-Ham's F12 (DMEM-F12) at 4 degrees C for 30- to 60 minutes or 12hours. The growth dynamics of PDL fibroblasts were evaluated with cell proliferation graphics and population doubling time (PDT) values. Runt-related transcription factor 2 (RUNX2), receptor activator of nuclear factor kappa-B ligand (RANKL), and collagen type XII (COL12) expression were used to evaluate the differentiation of PDL fibroblasts. ResultsThe percentage of cell numbers and PDT values of groups were statistically insignificant. In the HBSS groups, RUNX2 expression increased showing a direction to osteogenic differentiation of PDL fibroblasts. In the DMEM-F12 groups, RANKL expression increased, indicating there was a tendency for osteoclastogenic differentiation. In the milk groups, RUNX2 expression decreased while other markers were stable showing PDL fibroblasts could protect fibroblast identity. ConclusionsIn terms of protecting fibroblast identity and resistance to differentiation, milk was more effective than HBSS.Öğe Öğe Isolation Methods of Dental Pulp Stem Cells(SPRINGER, 2013) Yildirim, Sibel[Abstract not Available]Öğe Nonsurgical endodontic treatments in molar teeth with large periapical lesions in children: 2-year follow-up(MOSBY-ELSEVIER, 2007) Kusgoz, Adem; Yildirim, Sibel; Gokalp, AlparslanProfound caries lesions may lead to invasion of microorganisms to the dental pulp, and periapical areas can promote the development of dentoalveolar abscess and periapical bone loss. Treatment options to manage large periapical lesions range from nonsurgical root canal treatment and/ or apical surgical procedure to extraction. Young molar teeth with pulp necrosis and large periapical lesions in children are frequently treated with root canal treatment because the therapy is more difficult in multirooted teeth. In these case reports, nonsurgical endodontic treatments performed on 6 molar teeth with large periapical lesions, by repeated intracanal dressing with calcium hydroxide, are presented. Radiographs displayed significant bony healing at the end of the second year. In conclusion, large periapical lesions can respond favorably to nonsurgical treatment, and complex and difficult endodontic treatments in children might not be required.Öğe Reprogramming of DPSC to Induced Pluripotent Stem Cells(SPRINGER, 2013) Yildirim, Sibel[Abstract not Available]Öğe
