A Simple Semi-preparative Reversed-phase HPLC/PDA Method for Separation and Quantification of Glycyrrhizin in Nine Samples of Glycyrrhiza glabra Root Collected from Different Geographical Origins

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dc.contributor.authorBasar, Norazah
dc.contributor.authorTalukdar, Anupam D.
dc.contributor.authorNahar, Lutfun
dc.contributor.authorStafford, Angela
dc.contributor.authorKushiev, Habibjon
dc.contributor.authorKan, Asuman
dc.contributor.authorSarker, Satyajit D.
dc.date.accessioned2020-03-26T18:49:16Z
dc.date.available2020-03-26T18:49:16Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIntroduction - Glycyrrhiza glabra L. (Fabaceae), commonly known as 'liquorice', is one of the most popular ingredients in several traditional herbal medicinal preparations, and glycyrrhizin is the major glycoside present in this plant. The content of glycyrrhizin may vary among G. glabra samples collected from various geographical origins, which may affect the therapeutic efficacy. Thus, quantification of glycyrrhizin in G. glabra samples is important. Objective - To develop and validate a simple semi-preparative reversed-phase HPLC with photodiode array (PDA) method for separation and quantification of glycyrrhizin in nine samples of G. glabra root collected from various geographical origins. Methods - Dried and ground root of G. glabra was Soxhlet-extracted sequentially with n-hexane and methanol (MeOH). The separation and quantification of glycyrrhizin was achieved on a C-18 reversed-phase semi-preparative column using a gradient mobile phase, 30-100% solvent B in solvent A in 30 min (solvent A: 0.1% v/v trifluoroacetic acid (TFA) in water and solvent B: 0.1% v/v of TFA in MeOH), at a flow rate of 3.00 mL/min and UV detection at 254 nm. Results - A simple semi-preparative reversed-phase HPLC/PDA method allowing clear separation and quantification of glycyrrhizin content in nine samples has been validated in terms of linearity, selectivity, limits of detection, precision, accuracy and detection. Concentration levels of glycyrrhizin were between 0.177 and 0.688% w/w of dry materials. Conclusion - This method is precise, less time consuming and more cost effective, and can be used for the quality control of any G. glabra sample with regard to its glycyrrhizin contents. Copyright (C) 2014 John Wiley & Sons, Ltd.en_US
dc.description.sponsorshipMinistry of Education (MOE), Malaysiaen_US
dc.description.sponsorshipThe authors acknowledge Ministry of Education (MOE), Malaysia for financial support (NB) and EPSRC National Mass Spectrometry Service Centre (Department of Chemistry, University of Wales Swansea, Swansea, Wales, UK) for the MS analysis of glycyrrhizin.en_US
dc.identifier.doi10.1002/pca.2507en_US
dc.identifier.endpage404en_US
dc.identifier.issn0958-0344en_US
dc.identifier.issn1099-1565en_US
dc.identifier.issue5en_US
dc.identifier.pmid24585378en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage399en_US
dc.identifier.urihttps://dx.doi.org/10.1002/pca.2507
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30570
dc.identifier.volume25en_US
dc.identifier.wosWOS:000341149500002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofPHYTOCHEMICAL ANALYSISen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHPLC/PDAen_US
dc.subjectsemi-preparativeen_US
dc.subjectliquoriceen_US
dc.subjectglycyrrhizinen_US
dc.subjectGlycyrrhiza glabraen_US
dc.titleA Simple Semi-preparative Reversed-phase HPLC/PDA Method for Separation and Quantification of Glycyrrhizin in Nine Samples of Glycyrrhiza glabra Root Collected from Different Geographical Originsen_US
dc.typeArticleen_US

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