Visualisation of the Uptake of Prolactin (PRL) in Rat Embryonic Tissues

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dc.contributor.authorKarabulut, A. K.
dc.contributor.authorUlger, H.
dc.contributor.authorPratten, M. K.
dc.date.accessioned2020-03-26T16:27:43Z
dc.date.available2020-03-26T16:27:43Z
dc.date.issued1999
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractObjective: Evidences implicate roles for prolactin (PRL) in the regulation of embryonic growth. To clarify the roles of PRL in rat embryogenesis we examined the uptake and expression of the hormone in embryonic tissues. Methods: Nine and a half day postimplantation rat embryos were cultured in vitro for 44 h in rat serum and serum depleted of low molecular weight molecules (retenate). The embryos were transferred to M199 for the last 4 h, and 12.8 ng/ml rat PRL was added to culture medium for different times (4 h - 15 min) and/or different temperatures (37°C and 4°C). As a control tissue, pituitary glands from 11.5 and 18.5d pregnant rats were used. Embryos and tissues were then examined by an indirect immunofluorescence protocol. Results: The pituitary glands showed positive immunoreactivity for anti-PRL antibody whilst there was no stain in the control brain tissue. Immunoreactivity was observed in embryos grown in rat serum, and intensity was much greater in the presence of additional rat PRL, whilst there was no immunoreactivity detected in those grown in retenate only. Shorter incubations and incubations at 37°C caused a greater immunoreactivity for PRL, suggesting that this is an active and temperature dependent metabolic process. Conclusion: These results show the uptake and distribution of PRL by the yolk sac and embryonic tissues which might be interpreted for the presence of PRL receptors.en_US
dc.identifier.citationKarabulut, A. K., Ulger, H., Pratten, M. K., (1999). Visualisation of the Uptake of Prolactin (PRL) in Rat Embryonic Tissues. Marmara Medical Journal, 12(1), 11-19.
dc.identifier.endpage19en_US
dc.identifier.issn1019-1941en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage11en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/17045
dc.identifier.volume12en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKarabulut A.K.
dc.language.isoenen_US
dc.relation.ispartofMarmara Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectDevelopmenten_US
dc.subjectEmbryo cultureen_US
dc.subjectOrganogenesisen_US
dc.subjectProlactin receptorsen_US
dc.titleVisualisation of the Uptake of Prolactin (PRL) in Rat Embryonic Tissuesen_US
dc.typeArticleen_US

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