Selection of reliable reference genes for qRT-PCR analysis on head and neck squamous cell carcinomas

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dc.contributor.authorYiğin, Aysel Kalaycı
dc.contributor.authorÇora, Tülin
dc.contributor.authorAcar, Hasan
dc.contributor.authorKurar, Ercan
dc.contributor.authorKayış, S. A.
dc.contributor.authorÇolpan, Bahar
dc.contributor.authorÖztürk, Kayhan
dc.contributor.authorHitit, Mustafa
dc.date.accessioned2020-03-26T19:43:57Z
dc.date.available2020-03-26T19:43:57Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe choice of reliable reference genes as an internal control is inevitable to obtain accurate results. Here we present an assessment of 7 reference genes (18S rRNA, 28S rRNA, ACTB, GAPDH, TUBA1, YWHAZ, and SDHA) to normalize gene expression data in Head and Neck Squamous Cell Carcinomas (HNSCCs). We attempted to determine a reliable set of reference genes to use in the normalization of gene expression data in Head and Neck Squamous Cell Carcinomas (HNSCCs) and normal mucosal tissues. Malignant and non-malignant tissue samples were collected from 12 patients with primary untreated HNSCC. geNorm and NormFinder software packages were used for data evaluations. Results obtained by geNorm indicated that average expression stability values (M) of all candidates genes were smaller than 1.5 (accepted M value for geNorm), showing that all the evaluated genes can be employed as HKGs, although GAPDH and ACTB were reported to be the most stable. Similarly, NormFinder results were in agreement with geNorm’s results. GAPDH and ACTB are considered to be most suitable reference genes to evaluate novel gene expression in the tissues several of HNSCCs. © 2017, Scientific Publishers of India. All rights reserved.en_US
dc.identifier.endpage2018en_US
dc.identifier.issn0970938Xen_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage2014en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35790
dc.identifier.volume28en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherScientific Publishers of Indiaen_US
dc.relation.ispartofBiomedical Research (India)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGeNormen_US
dc.subjectHead and neck squamous cell carcinomaen_US
dc.subjectHousekeeping geneen_US
dc.subjectNormFinderen_US
dc.titleSelection of reliable reference genes for qRT-PCR analysis on head and neck squamous cell carcinomasen_US
dc.typeArticleen_US

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