Comparison of ?-glucosyl hesperidin of citrus fruits and epigallocatechin gallate of green tea on the Loss of Rotavirus Infectivity in Cell Culture

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dc.contributor.authorLipson S.M.
dc.contributor.authorOzen F.S.
dc.contributor.authorLouis S.
dc.contributor.authorKarthikeyan L.
dc.date.accessioned2020-03-26T19:08:14Z
dc.date.available2020-03-26T19:08:14Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractA number of secondary plant metabolites (e.g., flavonoids) possess antiviral/antimicrobial activity. Most flavonoids, however, are difficult to study, as they are immiscible in water-based systems. The relatively new semisynthetic ?-glucosyl hesperitin (GH), and the natural plant product epigallocatechin gallate (EGCG) are unique among most flavonoids, as these flavonoids are highly soluble. The antiviral activity of these plant metabolites were investigated using the rotavirus as a model enteric virus system. Direct loss of virus structural integrity in cell-free suspension and titration of amplified RTV in host cell cultures was measured by a quantitative enzyme-linked immunosorbent assay (qEIA). After 30 min. 100 × 103 ?g/ml GH reduced RTV antigen levels by ca. 90%. The same compound reduced infectivity (replication in cell culture) by a similar order of magnitude 3 to 4 days post inoculation. After 3 days in culture, EGCG concentrations of 80, 160, and 320 ?g/ml reduced RTV infectivity titer levels to ca. 50, 20, and 15% of the control, respectively. Loss of RTV infectivity titers occurred following viral treatment by parallel testing of both GH and EGCG, with the latter, markedly more effective. Cytotoxicity testing showed no adverse effects by the phenolic concentrations used in this study. The unique chemical structure of each flavonoid rather than each phenolic's inherent solubility may be ascribed to those marked differences between each molecule's antiviral (anti-RTV) effects. The solubility of EGCG and GH obviated our need to use potentially confounding or obfuscating carrier molecules (e.g., methanol, ethanol, DMSO) denoting our use of a pure system environ. Our work further denotes the need to address the unique chemical nature of secondary plant metabolites before any broad generalizations in flavonoid (antiviral) activity may be proposed. © 2015 Lipson, Ozen, Louis and Karthikeyan.en_US
dc.identifier.doi10.3389/fmicb.2015.00359en_US
dc.identifier.issn1664302Xen_US
dc.identifier.issueAPRen_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://dx.doi.org/10.3389/fmicb.2015.00359
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32805
dc.identifier.volume6en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherFrontiers Research Foundationen_US
dc.relation.ispartofFrontiers in Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectA-glucosyl hesperidinen_US
dc.subjectELISAen_US
dc.subjectEpigallocatechin gallateen_US
dc.subjectInfectivity titersen_US
dc.subjectRotavirusen_US
dc.titleComparison of ?-glucosyl hesperidin of citrus fruits and epigallocatechin gallate of green tea on the Loss of Rotavirus Infectivity in Cell Cultureen_US
dc.typeArticleen_US

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