Exploring the interaction between small RNAs and R genes during Brachypodium response to Fusarium culmorum infection

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dc.contributor.authorLucas, Stuart James
dc.contributor.authorBastas, Kubilay
dc.contributor.authorBudak, Hikmet
dc.date.accessioned2020-03-26T18:50:42Z
dc.date.available2020-03-26T18:50:42Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe present study aims to investigate small RNA interactions with putative disease response genes in the model grass species Brachypodium distachyon. The fungal pathogen Fusarium culmorum (Fusarium herein) and phytohormone salicylic acid treatment were used to induce the disease response in Brachypodium. Initially, 121 different putative disease response genes were identified using bioinformatic and homology based approaches. Computational prediction was used to identify 33 candidate new miRNA coding sequences, of which 9 were verified by analysis of small RNA sequence libraries. Putative Brachypodium miRNA target sites were identified in the disease response genes, and a subset of which were screened for expression and possible miRNA interactions in 5 different Brachypodium lines infected,with Fusarium. An NBS-LRR family gene, 1g34430, was polymorphic among the lines, forming two major genotypes, one of which has its miRNA target sites deleted, resulting in altered gene expression during infection. There were siRNAs putatively involved in regulation of this gene, indicating a role of small RNAs in the B. distachyon disease response. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipResearch Fellowship from the Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipDuring this work, SJL was supported by a Research Fellowship from the Scientific and Technological Research Council of Turkey (TUBITAK). The authors thank Fatma Aydinoglu and Dilay Cansever for practical assistance and Bala Am Akpinar for practical assistance and help in revising the manuscript.en_US
dc.identifier.doi10.1016/j.gene.2013.12.025en_US
dc.identifier.endpage264en_US
dc.identifier.issn0378-1119en_US
dc.identifier.issn1879-0038en_US
dc.identifier.issue2en_US
dc.identifier.pmid24368332en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage254en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.gene.2013.12.025
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30858
dc.identifier.volume536en_US
dc.identifier.wosWOS:000331021800005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofGENEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBrachypodium distachyonen_US
dc.subjectR genesen_US
dc.subjectNBS-LRRsen_US
dc.subjectPlant miRNAsen_US
dc.subjectPlant diseaseen_US
dc.titleExploring the interaction between small RNAs and R genes during Brachypodium response to Fusarium culmorum infectionen_US
dc.typeArticleen_US

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