Smart-Polymer-Functionalized Graphene Nanodevices for Thermo-Switch-Controlled Biodetection

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dc.contributor.authorBalcioglu, Mustafa
dc.contributor.authorBuyukbekar, Burak Zafer
dc.contributor.authorYavuz, Mustafa Selman
dc.contributor.authorYigit, Mehmet V.
dc.date.accessioned2020-03-26T19:07:07Z
dc.date.available2020-03-26T19:07:07Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this work, we have developed a general methodology for constructing an activatable biosensor utilizing a thermoresponsive polymer and two-dimensional nanosheet. We have demonstrated the detection of four different types of biological compounds using the smart PEGMA (poly(ethylene glycol) methyl ether methacrylate), oligonucleotides, and graphene oxide nanoassembly. The activity of the functional nanodevice is controlled with a thermo-switch at 39 degrees C. In this design, the nanosized graphene oxide serves as a template for fluorophore labeled probe oligonucleotides while quenching the fluorescence intensities dramatically. On the other hand, the PEGMA polymer serves as an activatable protecting layer covering the graphene oxide and entrapping the probe oligonucleotides on the surface. The PEGMA polymers are hydrophobic above their lower critical solution temperature (LCST) and therefore interact strongly with the hydrophobic surface of graphene oxide, creating a closed configuration (OFF state) of the nanodevice. However, once the temperature decreases below the LCST, the polymer undergoes conformational change and becomes hydrophilic. This opens up the surface of the graphene oxide (open configuration, ON state), freeing the encapsulated payload on the surface. We have tuned the activity of the nanodevice for the detection of a sequence-specific DNA, miR-10b, thrombin, and adenosine. The activity of our functional system can be decreased by 80% with a thermo-switch at 39 C. Our approach can be extended to other antisense oligonucleotide, aptamer, or DNAzyme based sensing strategies.en_US
dc.description.sponsorshipMinistry of National Education, Republic of TurkeyMinistry of National Education - Turkey; SUNY Albany Start-Up Fundsen_US
dc.description.sponsorshipWe acknowledge the Ministry of National Education, Republic of Turkey, for financial support to M.B. with a full fellowship during his doctoral studies. This work was supported by the SUNY Albany Start-Up Funds.en_US
dc.identifier.doi10.1021/ab500029hen_US
dc.identifier.endpage36en_US
dc.identifier.issn2373-9878en_US
dc.identifier.issue1en_US
dc.identifier.pmid33435080en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage27en_US
dc.identifier.urihttps://dx.doi.org/10.1021/ab500029h
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32560
dc.identifier.volume1en_US
dc.identifier.wosWOS:000369189900006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAMER CHEMICAL SOCen_US
dc.relation.ispartofACS BIOMATERIALS SCIENCE & ENGINEERINGen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectnanodeviceen_US
dc.subjectgrapheneen_US
dc.subjectthermoresponsive polymeren_US
dc.subjectPEGMAen_US
dc.subjectDNAen_US
dc.subjectmiRNAen_US
dc.subjectthrombinen_US
dc.subjectadenosineen_US
dc.subjectaptameren_US
dc.titleSmart-Polymer-Functionalized Graphene Nanodevices for Thermo-Switch-Controlled Biodetectionen_US
dc.typeArticleen_US

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