Role of the nitric oxide on diazoxide-induced relaxation of the calf cardiac vein and coronary artery during cooling

The effects of cooling (to 28 degrees C) on the vasodilation induced by diazoxide (10(-9)-3 x 10(-4) m) on carbachol-pre-contracted calf cardiac vein and coronary artery and the role of nitric oxide in these effects were analyzed. Diazoxide produced concentration-dependent relaxation of calf cardiac vein and coronary artery rings pre-contracted with carbachol (10(-6) m). During cooling, the pIC(50) values, but not the maximal responses, to diazoxide were significantly lower than at 37 degrees C in both preparations. Cooling to 28 degrees C in the presence of N(G)-nitro-L-arginine methyl ester (10(-4) m) did not modify the effect of temperature both in cardiac vein and coronary artery. These results suggest that cooling-induced changes of diazoxide in calf cardiac vein and coronary artery are independent of nitric oxide.