Detection of Brucella antibodies in sheep sera using dot-immunobinding assay and Rose Bengal plate agglutination test

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dc.contributor.authorGurturk, K
dc.contributor.authorIlhan, Z
dc.contributor.authorErganis, O
dc.date.accessioned2020-03-26T16:25:21Z
dc.date.available2020-03-26T16:25:21Z
dc.date.issued1997
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, a dot-immunobinding assay (DIA) using nitrocellulose membrane was performed for the detection of Brucella antibodies in 206 sheep sera. Extract of Brucella abortus strain S99 obtained by heat treatment was used as antigen. The results were compared with those of Rose Bengal plate agglutination test (RBPAT). Of the 96 sera collected from the 8 aborted sheep flocks, 46 were positive by both tests and 17 were positive only in DIA. Of the 110 sera collected from slaughtered sheep, 13 were positive by both tests and 4 were positive only in DIA. Of the total 206 Sera tested, 147 were negative by RBPAT and only 126 were negative in DIA. All the sera giving positive reactions in RBPAT were also positive in DIA. it is concluded that DIA would be useful to apply for the detection of Brucella antibodies in sheep sera.en_US
dc.identifier.endpage344en_US
dc.identifier.issn1300-0128en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage341en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/16572
dc.identifier.volume21en_US
dc.identifier.wosWOS:A1997XQ84100011en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEYen_US
dc.relation.ispartofTURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectdot-immunobinding assayen_US
dc.subjectRose Bengal plate agglutination testen_US
dc.subjectsheepen_US
dc.subjectbrucellosisen_US
dc.titleDetection of Brucella antibodies in sheep sera using dot-immunobinding assay and Rose Bengal plate agglutination testen_US
dc.typeArticleen_US

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