Phylogenetic analysis of Jurinea (Asteraceae) species from Turkey based on ISSR amplification

Jurinea remains a taxonomically complex genus, many of the problems concentrating around J. mollis. In this study, Jurinea species native in Turkey were collected from different localities in Anatolia and DNA was isolated from the collected samples, using a commercial plant DNA extraction kit (Nucleon phytopure). Inter Simple Sequence Repeat (ISSR) was the fingerprinting method preferred due to its high level of reliability and consistency as compared with the Randomly Amplified Polymorphic DNA (RAPD). The data were analyzed with NTSYS-pc 2.1. The phytogeographical distribution of Jurinea was found to be closely related to the Anatolian Diagonal. Based on the Diagonal, two major taxonomic groups emerged, from which J. kileae was clearly separated both morphologically and phylogenetically.