Comparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A viruses

Basit Öğe Kaydı
dc.contributor.authorSevik, Murat
dc.contributor.authorOzturk, Fahri Ferudun
dc.date.accessioned2020-03-26T18:41:19Z
dc.date.available2020-03-26T18:41:19Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, we compared 2 methods, liquid-phase blocking ELISA (LPBE) and solid-phase competition ELISA (SPCE), for the detection of antibodies to the structural proteins of foot-and-mouth disease virus (FMDV). These methods were compared using sera collected from cattle (n = 30) without a history of foot-and-mouth disease infection or vaccination, cattle (n = 180) vaccinated with oil-adjuvanted bivalent vaccine, and international reference sera (positive, weak positive, and negative) for FMDV serotypes O and A. The results showed that SPCE had a better specificity (96.67% for serotype O and 100% for serotype A) than LPBE (90% for serotype O and 93.33% for serotype A). Sensitivity of LPBE (97.22% for serotype O and 98.33% for serotype A) was almost equivalent to that of SPCE (98.33% for serotype O and 98.89% for serotype A). It can be concluded that SPCE is more suitable than LPBE for use as a screening test for the detection of antibodies against structural proteins of FMDV.en_US
dc.description.sponsorshipScientific Research Projects Coordination Center of Selcuk University, Konya, TurkeySelcuk University [09202025]en_US
dc.description.sponsorshipWe thank the FMD Institute (Ankara, Turkey) for the use of laboratory facilities. This study was summarized from a PhD thesis and was supported by the Scientific Research Projects Coordination Center of Selcuk University, Konya, Turkey (Project No: 09202025).en_US
dc.identifier.doi10.3906/vet-1303-26en_US
dc.identifier.endpage528en_US
dc.identifier.issn1300-0128en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage523en_US
dc.identifier.urihttps://dx.doi.org/10.3906/vet-1303-26
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29308
dc.identifier.volume37en_US
dc.identifier.wosWOS:000323607100007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherSCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAKen_US
dc.relation.ispartofTURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCattleen_US
dc.subjectfoot-and-mouth diseaseen_US
dc.subjectstructural proteinen_US
dc.subjectliquid-phase blocking ELISAen_US
dc.subjectsolid-phase competition ELISAen_US
dc.titleComparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A virusesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu