A comparative study for lipase immobilization onto alginate based composite electrospun nanofibers with effective and enhanced stability

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dc.contributor.authorDogac, Yasemin Ispirli
dc.contributor.authorDeveci, Ilyas
dc.contributor.authorMercimek, Bedrettin
dc.contributor.authorTeke, Mustafa
dc.date.accessioned2020-03-26T19:33:24Z
dc.date.available2020-03-26T19:33:24Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, lipase was successfully immobilized on polyvinyl alcohol/alginate and polyethylene oxide/alginate nanofibers that were prepared by electrospinning. Results showed that nanofibers (especially polyvinyl alcohol/alginate) enhanced the stability properties of lipase. When the free lipase lost its all activity after 40-60 min at high temperatures, both lipase immobilized nanofibers kept almost 65-70% activity at the same time. The lipase immobilized poly vinyl alcohol/alginate and polyethylene oxide/alginate nanofibers protected approximately all of their activities until pH 9. Lipase immobilized polyvinyl alcohol/alginate and polyethylene oxide/alginate nanofibers maintained 60% of their activities after 14 and 7 reuses, respectively. The morphology of nanofibers was characterized by Scanning Electron Microscope, Fourier Transform Infrared Spectroscopy and Thermal Gravimetric Analyzer. As a result, this nanofiber production method, electrospinning, is simple, versatile and economical for preparing appropriate carrier to immobilize the enzymes. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipMugla Sitki Kocman University Scientific Research ProjectMugla Sitki Kocman University [13/182]en_US
dc.description.sponsorshipThis work was supported by a grant from the Mugla Sitki Kocman University Scientific Research Project (No: 13/182).en_US
dc.identifier.doi10.1016/j.ijbiomac.2016.11.120en_US
dc.identifier.endpage311en_US
dc.identifier.issn0141-8130en_US
dc.identifier.issn1879-0003en_US
dc.identifier.pmid27932259en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage302en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ijbiomac.2016.11.120
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34711
dc.identifier.volume96en_US
dc.identifier.wosWOS:000393245700034en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectNanofiberen_US
dc.subjectElectrospinningen_US
dc.subjectImmobilizationen_US
dc.subjectLipaseen_US
dc.subjectCompositeen_US
dc.subjectAlginateen_US
dc.titleA comparative study for lipase immobilization onto alginate based composite electrospun nanofibers with effective and enhanced stabilityen_US
dc.typeArticleen_US

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