New Amperometric Cholesterol Biosensors Using Poly(ethyleneoxide) Conducting Polymers

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dc.contributor.authorYıldız, Hüseyin Bekir
dc.contributor.authorDemirkol, Dilek Odacı
dc.contributor.authorSayın, Serkan
dc.contributor.authorYılmaz, Mustafa
dc.contributor.authorKöysüren, Özcan
dc.contributor.authorKamacı, Musa
dc.date.accessioned2020-03-26T18:42:37Z
dc.date.available2020-03-26T18:42:37Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAccumulation of cholesterol in human blood can cause several health problems such as heart disease, coronary artery disease, arteriosclerosis, hypertension, cerebral thrombosis, etc. Therefore, simple and fast cholesterol determination in blood is clinically important. In this study, two types of amperometric cholesterol biosensors were designed by physically entrapping cholesterol oxidase in conducting polymers; thiophene capped poly(ethyleneoxide)/polypyrrole (PEO-co-PPy) and 3-methylthienyl methacrylate-co-p-vinyl benzyloxy poly(ethyleneoxide)/polypyrrole (CP-co-PPy). PEO-co-PPy and CP-co-PPy were synthesized electrochemically and cholesterol oxidase was immobilized by entrapment during electropolymerization. The amperometric responses of the enzyme electrodes were measured by monitoring oxidation current of H2O2 at +0.7 V in the absence of a mediator. Kinetic parameters, such as Km and Imax, operational and storage stabilities, effects of pH and temperature were determined for both entrapment supports. Km values were found as 1.47 and 5.16 mM for PEO-co-PPy and CP-co-PPy enzyme electrodes, respectively. By using these Km values, it can be observed that ChOx immobilized in PEO-co-PPy shows higher affinity towards the substrate.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109T439]; Scientific Research Projects Foundation of Karamanoglu Mehmetbey University (KMU-BAP)Karamanoglu Mehmetbey University [09-M-11]en_US
dc.description.sponsorshipAuthors would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK Grant Number 109T439) and the Scientific Research Projects Foundation of Karamanoglu Mehmetbey University (KMU-BAP Grant Number 09-M-11) for the financial support of this research.en_US
dc.identifier.doi10.1080/10601325.2013.821921en_US
dc.identifier.endpage1084en_US
dc.identifier.issn1060-1325en_US
dc.identifier.issn1520-5738en_US
dc.identifier.issue10en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1075en_US
dc.identifier.urihttps://dx.doi.org/10.1080/10601325.2013.821921
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29672
dc.identifier.volume50en_US
dc.identifier.wosWOS:000324345900008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS INCen_US
dc.relation.ispartofJOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectcholesterol oxidaseen_US
dc.subjectAmperometric biosensorsen_US
dc.subjectenzyme immobilizationen_US
dc.subjectconducting polymersen_US
dc.subjectcholesterol sensorsen_US
dc.titleNew Amperometric Cholesterol Biosensors Using Poly(ethyleneoxide) Conducting Polymersen_US
dc.typeArticleen_US

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