Influence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen

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dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorBodu, Mustafa
dc.contributor.authorBaspinar, Nuri
dc.contributor.authorGungor, Sukru
dc.contributor.authorIli, Pinar
dc.contributor.authorAcibaeva, Begimay
dc.contributor.authorTopraggaleh, Tohid Rezaei
dc.contributor.authorDursun, Şükrü
dc.date.accessioned2020-03-26T20:14:45Z
dc.date.available2020-03-26T20:14:45Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Bilimler Bölümüen_US
dc.description.abstractObjective: The purpose of the present study was to assess the effects of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen. Materials and Methods: In this experimental study, sixty ejaculates from six mature Merino rams were used. In experiment 1, the ejaculates were diluted in base extender contained ellagic acid at 0 (control), 0.5, 1, and 2 mM. In experiment 2, ebselen at 0 (control), 10, 20, and 40 mu M were added to the extender. Sperm motility, viability, mitochondrial membrane potential, DNA integrity, lipid peroxidation (LPO), the antioxidant potential (AOP), and total glutathione (tGSH) were evaluated at 0, 24, 48, and 72 hours of preservation. Results: Supplementation of ellagic acid at 1 and 2 mM resulted in higher sperm motility and viability at 0 hours of storage. Ellagic acid at 2 mM led to higher motility and viability compared to controls after 0, 24, and 48 hours of preservation and increased AOP after 24 and 72 hours. Higher tGSH was at 1 mM ellagic acid, compared to control after 72 hours. Addition of ebselen at a concentration of 40 mu M increased motility at 24 and 48 hours and 10 mu M produced the same effect after 48 and 72 hours of storage as well as higher viability, compared to the controls after 0 hours of storage. Sperm DNA integrity was significantly improved after 24, 48, and 72 hours with the addition of ebselen at 10 mu M, and after 72 hours at 40 mu M. Addition of 40 mM ebselen also reduced the LPO levels after 24 hours of storage compared to the controls. Conclusion: The results showed that supplementation of ellagic acid and ebselen in semen extender has a potential effect on sperm and oxidative stress parameters during liquid preservation of ram semen.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112 O 845]en_US
dc.description.sponsorshipThis study was financially supported by Scientific and Technological Research Council of Turkey (TUBITAK) (Project No: 112 O 845). There is no conflict of interest in this study.en_US
dc.identifier.citationBucak, M. N., Bodu, M., Başpınar, N., Güngör, Ş., İli, P., Acibaeva, B., Topraggaleh, T. R., Dursun, Ş. (2019). Influence Of Ellagic Acid And Ebselen On Sperm And Oxidative Stress Parameters During Liquid Preservation Of Ram Semen. Cell Journal (Yakhteh), 21,(1), 7.
dc.identifier.doi10.22074/cellj.2019.5593en_US
dc.identifier.endpage13en_US
dc.identifier.issn2228-5806en_US
dc.identifier.issn2228-5814en_US
dc.identifier.issue1en_US
dc.identifier.pmid30507083en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage7en_US
dc.identifier.urihttps://dx.doi.org/10.22074/cellj.2019.5593
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37929
dc.identifier.volume21en_US
dc.identifier.wosWOS:000468781400002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBucak, Mustafa Numan
dc.institutionauthorBodu, Mustafa
dc.institutionauthorBaspinar, Nuri
dc.language.isoenen_US
dc.publisherROYAN INSTen_US
dc.relation.ispartofCELL JOURNALen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEbselenen_US
dc.subjectEllagic Aciden_US
dc.subjectPreservationen_US
dc.subjectRamen_US
dc.subjectSperm Parametersen_US
dc.titleInfluence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semenen_US
dc.typeArticleen_US

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