Comparison of newly developed hydroxyl-functionalized monodisperse HILIC columns new HILIC column

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dc.contributor.authorDinc, Saliha
dc.contributor.authorOlmez, Saba Samatya
dc.contributor.authorTuncel, Ali
dc.date.accessioned2020-03-26T19:34:30Z
dc.date.available2020-03-26T19:34:30Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, new hydroxyl-functionalized monodisperse polymeric hydrophilic interaction chromatography (HILIC) columns were developed using different derivatization agents. In addition, the influences of derivatization temperature of the best agent and polymer composition on the separation were investigated under HILIC conditions. Monodisperse-porous hydrophilic particles were synthesized by the seeded polymerization method using 3-chloro-2-hydroxypropile methacrylate (HPMA-CL) and ethylene glycol dimethacrylate (EGDMA) monomers. The chloropropyl terminal ends of the poly(HPMA-Cl-co-EGDMA) particles were derivatized with amine group of ethanolamine (EA), diethanolamine, and triethanolamine (TEA) at 80 degrees C through nucleophilic reaction. The performance of synthesized particles was evaluated with the amount of ligand on the particle surfaces, column backpressure, and separation power under HILIC condition. TEA was found to be the best derivatization agent for the separation of toluene, acrylamide, thymine, adenine, and cytosine in respect to resolution factors (>1.5 for all analytes) and theoretical plate numbers (64.562N/m for acylamide). Upon determination of the best ligand, then the effect of different derivatization temperatures and polymer composition on TEA performance was investigated. Of all the tested polymer compositions, the chromatographic performance of TEA-M-80 (the derivatization of TEA at 80 degrees C together with M polymer composition) was found to be the best.en_US
dc.identifier.doi10.1080/10826076.2017.1343731en_US
dc.identifier.endpage655en_US
dc.identifier.issn1082-6076en_US
dc.identifier.issn1520-572Xen_US
dc.identifier.issue12en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage649en_US
dc.identifier.urihttps://dx.doi.org/10.1080/10826076.2017.1343731
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34907
dc.identifier.volume40en_US
dc.identifier.wosWOS:000414217300005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS INCen_US
dc.relation.ispartofJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectDerivatization conditionsen_US
dc.subjectdifferent ligandsen_US
dc.subjecthydrophilic interaction chromatographyen_US
dc.subjecthydroxyl functionalizeden_US
dc.subjecttriethanolamineen_US
dc.titleComparison of newly developed hydroxyl-functionalized monodisperse HILIC columns new HILIC columnen_US
dc.typeArticleen_US

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