The Growth Promoting Effects of bFGF, PD-ECGF and Vegf on Cultured Postimplantation Rat Embryos Deprived of Serum Fractions

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dc.contributor.authorUlger, Harun
dc.contributor.authorKarabulut, Ahmet K.
dc.contributor.authorPratten, Margaret K.
dc.date.accessioned2020-03-26T16:36:21Z
dc.date.available2020-03-26T16:36:21Z
dc.date.issued2000
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractSerum components in which embryos are cultured in vitro are very important for normal embryonic development. In this study, rat serum was fractionated using Macrosep filters to study the effect of a single growth factor. The fractionated serum, both that containing only material greater than 30 kDa molecular weight (> 30 kDa) and that from which material between 30 kDa and 50 kDa had been removed (< 30 kDa + > 50 kDa), caused significant embryonic growth retardation. Addition of different concentrations of basic fibroblast growth factor (bFGF, 18 kDa), vascular endothelial growth factor (VEGF, 45 kDa) and platelet-derived endothelial growth factor (PD-ECGF, 45 kDa), to fractionated serum (bFGF to > 30 kDa serum and VEGF or PD-ECGF to < 30 kDa + > 50 kDa serum) partially restored embryonic growth and development according to a morphological scoring system and protein assay. This restoration was clear by all criteria, as well as in yolk sac vascularisation and heart development. The growth promoting effects of all 3 factors were significant but did not reach the level seen in embryos grown in whole rat serum. The effect of these growth factors was also investigated on anembryonic yolk sac development using a concentration for which maximum whole embryonic growth was seen (128 ng/ml bFGF, 1.6 ng/ml VEGF and 4 ng/ml PD-ECGF), and significant anembryonic yolk sac development was found. These findings suggest that the angiogenic factors may have a growth promoting effect on total embryonic development and vascularisation.en_US
dc.identifier.citationUlger, H., Karabulut, A. K., Pratten, M. K., (2000). The Growth Promoting Effects of bFGF, PD-ECGF and Vegf on Cultured Postimplantation Rat Embryos Deprived of Serum Fractions. Journal of Anatomy, (197), 207-219. Doi: 10.1046/j.1469-7580.2000.19720207.x
dc.identifier.doi10.1046/j.1469-7580.2000.19720207.xen_US
dc.identifier.endpage219en_US
dc.identifier.issn0021-8782en_US
dc.identifier.issn1469-7580en_US
dc.identifier.pmid11005713en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage207en_US
dc.identifier.urihttps://dx.doi.org/10.1046/j.1469-7580.2000.19720207.x
dc.identifier.urihttps://hdl.handle.net/20.500.12395/17267
dc.identifier.volume197en_US
dc.identifier.wosWOS:000089306100007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKarabulut, Ahmet K.
dc.language.isoenen_US
dc.publisherWıleyen_US
dc.relation.ispartofJournal of Anatomyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectangiogenic factorsen_US
dc.subjectwhole embryo cultureen_US
dc.subjectrat yolk sac cultureen_US
dc.subjectgrowthen_US
dc.titleThe Growth Promoting Effects of bFGF, PD-ECGF and Vegf on Cultured Postimplantation Rat Embryos Deprived of Serum Fractionsen_US
dc.typeArticleen_US

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