EFFICIENT REGENERATION AND AGROBACTERIUM TUMEFACIENS MEDIATED GENETIC TRANSFORMATION OF POTATO (SOLANUM TUBEROSUM L.)

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dc.contributor.authorAhmed, Hussein Abdullah
dc.contributor.authorBarpete, Surendra
dc.contributor.authorAkdoğan, Güray
dc.contributor.authorAydın, Gülsüm
dc.contributor.authorSancak, Cengiz
dc.contributor.authorÖzcan, Sebahattin
dc.date.accessioned2020-03-26T19:53:43Z
dc.date.available2020-03-26T19:53:43Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractPotato (Solanum tuberosum L.) is highly demandable world food crop and has become a staple food in many parts of the world including Turkey, India and China. Potato under in vitro culture condition is recalcitrant and genotype dependent. Therefore, we tested several factors to enhance regeneration and genetic transformation efficiency of Turkish potato genotypes. The influence of physical factors and conditions in Agrobacterium mediated transformation of the potato leaves was tested. Using the best combination (10 mg L-1 ZR, 10 mg L-1 NAA) with Agrobacterium-mediated transformation, we regenerated independent putative transformants 61.6% of the total explants cultured. GUS and nptII positive plants were confirmed by molecular analysis (PCR) that showed transformation rate of 24.16%. The protocol described here is simple, efficient, and produces transgenic plant in 7-8 weeks after inoculation with Agrobacterium bacterium.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThe present work is partially supported by the Scientific and Technological Research Council of Turkey (TUBITAK). The authors would like to thank Prof. Dr. Gungor Yilmaz (University of Gaziosmanpasa, Department of Field Crops) and Dr. Huseyin Onaran (Nigde Potato Research Institute) for providing the plant material. The authors would like to thank Dr Jitendra Kumar (Department of Plant Pathology, University of Minnesota, USA) for valuable suggestion and improvement of the present manuscript.en_US
dc.identifier.endpage3027en_US
dc.identifier.issn1018-4619en_US
dc.identifier.issn1610-2304en_US
dc.identifier.issue5en_US
dc.identifier.startpage3020en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/36576
dc.identifier.volume27en_US
dc.identifier.wosWOS:000436522600048en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherPARLAR SCIENTIFIC PUBLICATIONS (P S P)en_US
dc.relation.ispartofFRESENIUS ENVIRONMENTAL BULLETINen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectSolanum tuberosumen_US
dc.subjectAgrobacterium tumefaciensen_US
dc.subjectTransformationen_US
dc.subjectpotatoen_US
dc.subjectCo-cultivationen_US
dc.titleEFFICIENT REGENERATION AND AGROBACTERIUM TUMEFACIENS MEDIATED GENETIC TRANSFORMATION OF POTATO (SOLANUM TUBEROSUM L.)en_US
dc.typeArticleen_US

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