Seroprevalence of viral upper respiratory infections in dairy cattle

Bu araştırmada, İç Anadolu Bölgesi’nde yer alan Konya ve çevresindeki solunum sistemi semptomu gösteren süt sığırlarında Infectious Bovine Rhinotracheitis (IBR), Bovine Viral Diarrhea Virus (BVDV), Bovine Respiratory Syncytial Virus (BRSV), Parainfluenza Virus tip 3 (PI-3) ve Bovine Adenovirus tip 3 (BAV-3) enfeksiyonlarının seroprevalansı araştırıldı. Bu amaçla, özel çiftliklerde bulunan 1 yaş ve üzeri 5800 hayvan tarandı ve solunum sistemi hastalık semptomu gösteren, vücut ısısı normalin üstünde olan 278 hayvandan kan örneği alındı. Bu örnekler IBR, BVDV, BRSV, PI-3 ve BAV-3’e karşı antikorların varlığı yönünden ticari olarak temin edilen Enzyme Linked Immunosorbent Assay (ELISA) ile test edildi. Sığırlarda 5 virusa (IBR, BVDV, BRSV, PI- 3 ve BAV-3) karşı tespit edilen seroprevalans sırasıyla, %35.25, %96.04, %94.40, %92.80 ve %85.97 olarak saptandı. Diğer taraftan, örneklenen süt sığırlarının % 0.35’inde (1 hayvan) araştırmada kullanılan viruslara karşı antikor tespit edilmezken, %1.43’ ünde (4 hayvan) yalnızca bir, %2.87’sinde (8 hayvan) iki, %14.02’sinde (39 hayvan) üç, %49.64’ünde (138 hayvan) dört ve % 31.65’ inde (88 hayvan) ise beş virusa karşı antikor varlığı belirlendi. Solunum sistemi problemi olan süt sığırı işletmelerinde IBR, BVDV, BRSV, PI-3 ve BAV-3 enfeksiyonlarının yaygın ve seroprevalanslarının yüksek düzeyde seyrettiği belirlendi.

In this research, dairy cattle, located in Konya in Central Anatolian Region and its surrounding and had respiratory system symptoms, were investigated for the seroprevalence Infectious Bovine Rhinotracheitis (IBR), Bovine Viral Diarrhea Virus (BVDV), Bovine Respiratory Syncytial Virus (BRSV), Parainfluenza Virus type 3 (PI-3) and Bovine Adenovirus type 3 (BAV-3). For that purpose, 5800 animals, one year old and over, and bred in the private farms, were investigated and the blood samples were collected from 278 animals with respiratory system disease symptoms and high body temperatures. These samples were tested for presence of antibodies against IBR, BVDV, BRSV, PI-3 virus and BAV-3 by ELISA that was bought commercially. The seroprevalences which were determined for 5 viruses in cattle -(IBR, BVDV, BRSV, PI-3 virus and BAV-3)- were 35.25%, 96.04%, 94.40%, 92.80% and 85.97%, respectively. On the other hand, 0.35% (1 animal) of the dairy cattle sampled did not have any antibodies against the viruses. The existence of antibodies against the viruses were as such; 1.43% of cattle (4 animals) had antibodies against only one virus, 2.87% of cattle (8 animals) had antibodies against two, 14.02% of cattle (39 animals) had antibodies against three, 49.64% of cattle (138 animals) had antibodies against four and 31.65% of cattle (88 animals) had antibodies against five viruses. It was determined that IBR, BVDV, BRSV, PI-3 and BAV-3 infections were widespread and seroprevalences were high.