Multi-targeted potential of Pittosporum senacia Putt.: HPLC-ESI-MSn analysis, in silico docking, DNA protection, antimicrobial, enzyme inhibition, anticancer and apoptotic activity

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dc.contributor.authorMahomoodally, Mohamad Fawzi
dc.contributor.authorPicot-Allain, Carene
dc.contributor.authorHosenally, M.
dc.contributor.authorUğurlu, Aslı
dc.contributor.authorMollica, Adriano
dc.contributor.authorStefanucci, Azzurra
dc.contributor.authorLlorent-Martinez, E. J.
dc.contributor.authorBaloğlu, Mehmet Cengiz
dc.contributor.authorZengin, Gökhan
dc.date.accessioned2020-03-26T20:15:21Z
dc.date.available2020-03-26T20:15:21Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractPittosporum senacia (PS) Putt. (Pittosporaceae), indigenous to the Mascarene Islands, is a common ingredient in traditional medicines. However, there is currently a dearth of studies to validate some of these traditional claims. Given the broad traditional uses of PS against several diseases, we aimed to provide a comprehensive insight into the biological and chemical profile of P. senacia. The antioxidant, enzyme inhibitory activity, anticancer, and phytochemical composition of the methanolic extract of P. senacia leaf extracts were studied. The possible interaction and binding mode of the most abundant phytochemicals were studied via in silico docking experiments on tyrosinase and alpha-glucosidase. The mechanism behind the cytotoxic property of P. senacia extract for MDA-MB-231 was also examined using different methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability test checking apoptosis-associated genes, and wound healing assays. Twenty-six compounds were identified, of which caffeoylquinic acid derivatives, ferulic acid derivative, cinnamoylquinic acid derivative and two other polyphenols (oleuropeine and isoramnetin glucoside) being abundant, have been tested using in silico studies, against alpha-glucosidase and tyrosinase. The extract (IC50 = 118.8 mu g/ml) exhibited time and dose dependent anti-proliferative effect on human breast cancer cell line, MDA-MB-231. According to the expression profile of apoptosis inhibitors and apoptosis promoters genes, expression of Bar and Bak genes were significantly increased compared to Bcl-2 and Birc5 genes. Based on wound healing analysis, cell migration was inhibited after the application of the plant extract. The present findings suggested that PS might be a good candidate as sources of bioactive compounds for designing functional applications.en_US
dc.identifier.citationMahomoodally, M. F., Picot-Allain, C., Hosenally, M., Ugurlu, A., Mollica, A., Stefanucci, A., Llorent-Martinez, E. J.., Baloglu, M.C., Zengin, G. (2019). Multi-Targeted Potential Of Pittosporum Senacia Putt.: HPLC-ESI-Msn Analysis, İn Silico Docking, DNA Protection, Antimicrobial, Enzyme İnhibition, Anti-Cancer And Apoptotic Activity. Computational Biology And Chemistry, 83, 107114.
dc.identifier.doi10.1016/j.compbiolchem.2019.107114en_US
dc.identifier.issn1476-9271en_US
dc.identifier.issn1476-928Xen_US
dc.identifier.pmid31493741en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.compbiolchem.2019.107114
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38020
dc.identifier.volume83en_US
dc.identifier.wosWOS:000501651500023en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorZengin, Gokhan
dc.language.isoenen_US
dc.publisherELSEVIER SCI LTDen_US
dc.relation.ispartofCOMPUTATIONAL BIOLOGY AND CHEMISTRYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectPittosporaceaeen_US
dc.subjectMauritiusen_US
dc.subjectDiabetesen_US
dc.subjectAntimicrobialen_US
dc.subjectApoptosisen_US
dc.titleMulti-targeted potential of Pittosporum senacia Putt.: HPLC-ESI-MSn analysis, in silico docking, DNA protection, antimicrobial, enzyme inhibition, anticancer and apoptotic activityen_US
dc.typeArticleen_US

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