Characterisation of drug resistance of nosocomial ESBL-producing E. Coli isolates obtained from a Turkish university hospital between 2009 and 2012 by pulsed field gel electrophoresis and antibiotic resistance tests

Basit Öğe Kaydı
dc.contributor.authorKaragöz A.
dc.contributor.authorSunnetcioglu M.
dc.contributor.authorCeylan M.R.
dc.contributor.authorBayram Y.
dc.contributor.authorYalcin G.
dc.contributor.authorKocak N.
dc.contributor.authorSuvak B.
dc.date.accessioned2020-03-26T19:31:57Z
dc.date.available2020-03-26T19:31:57Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, drug resistance of 28 ESBL-producing Escherichia coli isolates obtained from 144 patients hospitalized at the Yüzüncüyil University Hospital at Van (YUH), Turkey, between 2009 and 2012 were characterized by pulsed field gel electrophoresis and antibiotic susceptibility tests. Antibiotic resistance profile was determined by a Phoenix automated system (BD, USA). The ratio of ESBL-producing E. coli strains was determined to be 19.4% (28 out of 144 E. coli isolates). It was determined that the anaesthesiology, paediatrics and thoracic medicine intensive care units in YUH were cross-contaminated between 2009 and 2012 by ESBL-producing E. coli strains, which is a sign of nosocomial infection in YUH. Analysis of PFGE results gave rise to two main PFGE profiles, profile-A with four subprofiles and profile-B with three subprofiles, where profile-A predominates over profile-B (14%). Comparison of the antibiotic resistance profile with the PFGE profile yielded similarities while some differences also exist due to either identical restriction enzyme cutting sites with slightly different genetic sequences in between the cutting sites or newly formed restriction enzyme cutting sites that do not affect antibiotic resistance genes. Enterobacteriaceae, particularly E. coli, have developed resistance in YUH by producing ESBLs against oxyimino and non-oxyimino cephalosporins, and penicillin-type antibiotics. Therefore, more effective antibiotics such as cefoxitin or cefoperazone- sulbactam should be used for the treatment of future nosocomial infections in YUH while hospital staff should take care with hygiene, such as hand washing. © 2016, EDIMES Edizioni Medico Scientifiche. All rights reserved.en_US
dc.identifier.endpage31en_US
dc.identifier.issn1124-9390en_US
dc.identifier.issue1en_US
dc.identifier.pmid27031893en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage24en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34256
dc.identifier.volume24en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherEDIMES Edizioni Medico Scientificheen_US
dc.relation.ispartofInfezioni in Medicinaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectE. colien_US
dc.subjectESBLen_US
dc.subjectPFGEen_US
dc.titleCharacterisation of drug resistance of nosocomial ESBL-producing E. Coli isolates obtained from a Turkish university hospital between 2009 and 2012 by pulsed field gel electrophoresis and antibiotic resistance testsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu