Investigation of direct toxic and teratogenic effects of anticoagulants on rat embryonic development using in vitro culture method and genotoxicity assay

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dc.contributor.authorUysal, İsmihan İlknur
dc.contributor.authorKarabulut, Ahmet Kağan
dc.contributor.authorÖzdemir, Kurtuluş
dc.contributor.authorAksoy, M.
dc.contributor.authorAltunkeser, Bülent Behlül
dc.contributor.authorAcar, Hasan
dc.date.accessioned2020-03-26T17:03:46Z
dc.date.available2020-03-26T17:03:46Z
dc.date.issued2006
dc.departmentSelçuk Üniversitesien_US
dc.description32nd Conference of the European-Teratology-Society -- SEP 19-22, 2004 -- Thessaloniki, GREECEen_US
dc.description.abstractHeparin and low molecular weight heparins (LMWHs) are used to reduce the incidence of venous thromboembolism in pregnancy. Although, these agents have been shown to be safe when used during pregnancy, the studies about direct toxic and teratogenic effects of these drugs on embryonic development are limited. In this study, the effects of heparin and LMWHs on rat embryonic development were investigated by using in vitro embryo culture and micronucleus (MN) assay methods. Rat embryos were cultured in vitro in the presence of different concentrations of heparin (5-40 IU/ml), dalteparin (2.5-20 IU/ml), enoxaparin (25-100 mu g/ml) and nadroparin (1-4 IU/ml). Effects of anticoagulants on embryonic developmental parameters were compared and embryos were evaluated for the presence of any malformations. After culturing the embryos, classic MN assay was performed. Anticoagulants significantly decreased all growth and developmental parameters dose-dependently. Dalteparin and enoxaparin were found to cause more developmental toxicity than heparin and nadroparin. Along with haematoma in general, heparin and nadroparin caused maxillary deformity, situs inversus and oedema most frequently, while neural tube defects were observed with dalteparin and enoxaparin. All agents also significantly induced MN formation in rat embryonic blood cells. These results indicate the possible genotoxic effects of anticoagulant agents on the developing rat embryo when applied directly.en_US
dc.description.sponsorshipEuropean Teratol Socen_US
dc.identifier.doi10.1111/j.1439-0264.2005.00642.xen_US
dc.identifier.endpage92en_US
dc.identifier.issn0340-2096en_US
dc.identifier.issn1439-0264en_US
dc.identifier.issue2en_US
dc.identifier.pmid16542172en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage84en_US
dc.identifier.urihttps://dx.doi.org/10.1111/j.1439-0264.2005.00642.x
dc.identifier.urihttps://hdl.handle.net/20.500.12395/20551
dc.identifier.volume35en_US
dc.identifier.wosWOS:000235937900003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofANATOMIA HISTOLOGIA EMBRYOLOGIAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleInvestigation of direct toxic and teratogenic effects of anticoagulants on rat embryonic development using in vitro culture method and genotoxicity assayen_US
dc.typeArticleen_US

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