Genetic characterization of Turkish cattle breeds by microsatellite markers: Usefulness for parentage testing

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dc.contributor.authorÖzşensoy, Yusuf
dc.contributor.authorKurar, Ercan
dc.contributor.authorDoğan, Müge
dc.contributor.authorBulut, Zafer
dc.contributor.authorNizamlıoğlu, Mehmet
dc.contributor.authorIşık, Ayşe
dc.contributor.authorÇamlıdağ, Aysun
dc.date.accessioned2020-03-26T18:50:53Z
dc.date.available2020-03-26T18:50:53Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Zootekni ve Hayvan Besleme Bölümüen_US
dc.description.abstractObjective of this study was to evaluate microsatellite markers in paternity testing of native cattle breeds in Turkey. Blood samples were collected from Anatolian Black (n=51), Anatolian Grey (n=54), South Anatolian Red (n=51), Native Southern Anatolian Yellow (n=51), East Anatolian Red (n=45) and Zavot (n=19) cattle. From the blood samples DNA was isolated by using a standard phenol/chloroform method. A total of 20 microsatellite loci were selected from a FAO/ISAG-suggested list. Polymerase chain reaction products were separated by capillary electrophoresis and marker genotypes were determined by fragment analysis. In statistical analyses, allel numbers, observed (Ho) and expected (He) heterozygosities, deviation from Hardy-Weinberg Equilibrium and probability of exclusion (PE) at each microsatellite locus were calculated. A total of 269 different alleles were observed and the mean allele was identified as 13.45. Mean Ho and He values were observed as 0.619-0.852 and 0.669-0.877, respectively. The results indicated that the microsatellite test panel including the most informative 7 loci had total PE value of >0.9999 in each populations and can thereby be used for parentage testing studies of native cattle breeds in Turkey.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [KAMAG - 106G114]; Selcuk University BAPSelcuk University [08202009]en_US
dc.description.sponsorshipThis research was supported by Scientific and Technological Research Council of Turkey (TUBITAK, KAMAG - 106G114) and Selcuk University BAP (08202009)en_US
dc.identifier.citationÖzşensoy, Y., Kurar, E., Doğan, M., Bulut, Z., Nizamlıoğlu, M., Işık, A., Çamlıdağ, A., Altunok, V. (2014). Genetic Characterization of Turkish Cattle Breeds by Microsatellite Markers: Usefulness for Parentage Testing. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 20(4), 521-526.
dc.identifier.doi10.9775/kvfd.2013.10454en_US
dc.identifier.endpage526en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage521en_US
dc.identifier.urihttps://dx.doi.org/10.9775/kvfd.2013.10454
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30889
dc.identifier.volume20en_US
dc.identifier.wosWOS:000344687800007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorKurar, Ercan
dc.institutionauthorDogan, Muge
dc.institutionauthorBulut, Zafer
dc.institutionauthorNizamlioglu, Mehmet
dc.language.isoenen_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCattleen_US
dc.subjectMicrosatelliteen_US
dc.subjectParentage testingen_US
dc.subjectTURKHAYGEN-Ien_US
dc.titleGenetic characterization of Turkish cattle breeds by microsatellite markers: Usefulness for parentage testingen_US
dc.typeArticleen_US

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