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Antioxidant and anticholinesterase effects of frequently consumed cereal grains using in vitro test models
(INFORMA HEALTHCARE, 2012) Senol, F. Sezer; Kan, Asuman; Coksari, Gulay; Orhan, Ilkay Erdogan
The ethyl acetate and ethanol extracts obtained from eight varieties (Faikbey, Y-1779, CI-8357, Cheokota, Seydisehir, Y-330, Sivas and YVD-18) of oat (Avena sativa L.), one variety (Larende) of barley (Hordeum vulgare L.), one variety (Tatlicak 97) of triticale (Triticale sp.) and one rye variety (Aslim 95) (Secale cereale L.) were investigated for their antioxidant effects in seven test systems. Anticholinesterase activity of the extracts was examined by enzyme-linked immunosorbent assay (ELISA) microplate reader. Total phenol and flavonoid contents were calculated using Folin Ciocalteau and AlCl3 reagents, respectively. All of the extracts were ineffective in cholinesterase inhibition assays and had weak-to-moderate activity in antioxidant assays. The extracts exerted better activity in iron-chelation capacity ranging between 43.17 +/- 2.04 and 62.97 +/- 1.29%. Triticale extracts showed higher activity in reducing power experiments. A notable difference in the results of the antioxidant activity assays was observed among the oat varieties.
Atomic absorption spectrometric analysis of Trigonella foenum-graecum L. seeds cultivated in Turkey
(2005) Kan, Yüksel; Kan, Asuman; Ceyhan, Timurhan; Sayar, Esin; Kartal, Murat; Altun, Levent; Aslan, Sinem
Trigonella foenum-graecum L. (Çemen) bitkisi Türkiye'de yaygın olarak yetişen iyi bilinen bir Leguminosae familyası bitkisidir ve bin yıldır baharat olarak, Çemen hazırlamada ve halk ilacı olarak kullanılmaktadır. Tohumların besleyici özelliklerinin olduğu ve sindirimi kolaylaştırdığı rapor edilmiştir. Bu çalışmada, farklı şartlarda kültüre alınmış Trigonella foenum- graecum bitkisinin tohumlarında bazı önemli mineraller (Al, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Ni, Pb and Zn) Atomik Absorbsiyon Spektrometriyöntemi ile ölçülmüştür.
Characterization of the Fatty Acid and Mineral Compositions of Selected Cereal Cultivars from Turkey
(ACG PUBLICATIONS, 2015) Kan, Asuman
In this present study, crude oil, fatty acid and mineral compositions of wheat (Triticum sp. L.), barley (Hordeum vulgare L.), triticale (Triticosecale Wittm. ex A. Camus.), rye (Secale cereale L.), and oat (Avena sativa L.) cultivars, respectively, from Turkey were investigated. Both the distribution of saturated fatty acids (SFA) and unsaturated fatty acids (UFA), and the mineral contents of evaluated cereals were reported. Fatty acid compositions and mineral contents were determined by gas chromatography/mass spectroscopy (GC/MS) and inductively coupled plasma optical emission spectroscopy (ICP-OES) techniques, respectively. The highest crude oil content was found in oat [cv. Seydisehir; 5.35%], whereas the lowest crude oil was in triticale [cv. Aslim-95; 1.19 %]. The results showed that the contents of total UFA in the different cultivars varied between 77.1 - 81.5 %. The major components of the cereal oils were determined as oleic and linoleic acid, respectively. The total macro-, micro-element, and heavy metal contents varied between 8638 -16108 ppm, 113-180 ppm and 1.8 - 6.9 ppm, respectively. As a conclusion, there were significant (p < 0.01) differences between the cereal cultivars in view of their crude oil contents, fatty acid and mineral compositions of the investigated samples from Turkey.
The Chemical Composition Profile of Dorystoechas hastataBoiss. & Heldr. Ex Bentham Cultivatedin Turkey
(ACG PUBLICATIONS, 2015) Kan, Asuman; Gunhan, Rabia Serpil; Celik, S. Ayse
Dorystoechas hastataBoiss. and Heldr. Ex Benthamis a monotypic plant endemic to Antalya province of Turkey. The chemical compositions of the water distilled essential oil of D. hastata different parts were investigated by GC/MS, which cultivated in Konya, Turkey. Three major compounds were identified from the aerial parts of D. hastata. Guaiol was the main component with 26.5 % of the branch essential oil. The leaf essential oil contained20.6 % 1,8-cineole, and the aerial parts contained15.0% borneol, respectively. Additionally, D. hastatacultivated samples were investigated for their macro (P, K, Ca, Mg, Na), micro (Fe, B, Mn, Zn, Cu) and heavy metal (Pb, Cd, Ni, Cr, Co) contents. The phosphorus (P) contents of D. hastata leaf, branch and the aerial parts were found as 3164, 2441 and 1852 ppm, respectively. Also, the amino acid compositions of different parts of D. hastata were investigated by HPLC. Major component of proteins in three different part of the plant was identified as proline. The highest proline content of D. hastata was found, however in the branch as 47.1 mg/100 g. As a conclusion, wihin this present study detailed chemical content of the cultivated D. hastata was investigated for the first time respective to its various parts.
EFFECT OF REDUCED AND INCREASED HERBICIDES DOSES ON WEED CONTROL STRATEGIES IN CHICKPEA (CICER ARIETINUM L.)
(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2018) Kaya, Muharrem; Sener, Aykut; Karaman, Ruziye; Atak, Mehmet; Kan, Asuman
This study was conducted to determine the effects of 50% reduced and 50% increased doses of some herbicides used in chickpea cultivation on seed yield and mineral content of the seed. Field experiments were established in Isparta province of Turkey during 2016-2017 plant growing season. Experiments were conducted to the completely randomized block design with 3 repetitions. Fourteen different weed control treatments were tested (control (T1), hoeing (T2) suggested doses and 50% reduced / increased doses of herbicides that contain active substances of linuron (T3-T5), aclonifen (T6-T8), pendimethalin (T9-T11) isoxaflutale + cyprosulfamide (T12-T14)), respectively. As plant material, drought and lodging tolerant ILC 195 chickpea cultivar seeds were used. Results showed that chlorophyll a, chlorophyll b content of green part of plants, phosphorus, potassium, calcium, iron, zinc, protein content of seeds, 100 seed weight and seed yield were significantly affected by the herbicides treatments. The highest positive effects on both the seed yield and the chemical composition of the seed were obtained at the dose of 125 ml/ha and 250 ml/ha of isoxaflutale + cyprosulfamide (240 g/l) and at 1500 ml/ha and 3000 ml/ha of pendimethalin herbicide treatments in chick pea cultivation.
The Elemental Characterization of Coriander Populations (Coriandrum Sativum L.) Cultivated in Turkey
(2017) Ayran, İrem; Çelık, Sadiye Ayşe; Kan, Asuman
Türkiye'de kültürü yapılan Şemsiyeçiçekliler (Umbelliferae) familyasına ait farklı kişniş populayonlarının mineral içerikleri (makro elementler: Na, K, Ca, Mg ve P; mikro elementler : Fe, Zn, Cu ve Mn; ağır metaller: Al, Ba, Co, Ni, Cr ve Pb) İndüktif Olarak Eşleşmiş Plazma-Optik Emüsyon Spektroskopisi (ICP-OES) ile belirlenmiştir. Kişniş populasyonlarının mineral içeriklerinin arasındaki farklılıklar istatistiki olarak önemli (p0.01) bulunmuştur. Populasyonlar içerisinde en yüksek fosfor (P) miktarı 7878.00 ppm ile Muğla populasyonundan elde edilirken en düşük 2969.91 ppm ile Tokat populasyonundan elde edilmiştir. Mikro elementlerden demir (Fe) içeriği en yüksek 435.00 ppm ile Kırşehir populasyonu iken en düşük 42.04 ppm ile Bursa yöresine ait populasyondan elde edilmiştir. Ağır metal içerikleri bakımından da populasyonlara göre en yüksek Alüminyum (Al) içeriği (850.91 ppm) Hatay populasyonundan elde edilmiştir. Bu çalışma ile, farklı kişniş populasyonlarının mineral içerikleri yeni çalışmalara katkı sunması amaçlanmıştır
Estimation of in vitro neuroprotective properties and quantification of rutin and fatty acids in buckwheat (Fagopyrum esculentum Moench) cultivated in Turkey
(ELSEVIER, 2012) Gulpinar, Ali Rifat; Orhan, Ilkay Erdogan; Kan, Asuman; Senol, Fatma Sezer; Celik, Sadiye Ayse; Kartal, Murat
Buckwheat (Fagopyrum esculentum Moench, Polygonaceae) is a popular food crop plant from Polygonaceae. In this study, we investigated the ethyl acetate and ethanol extracts of the seed, stem, and aerial parts of the plant for their neuroprotective effect through acetylcholinesterase, butyrylcholinesterase, and tyrosinase inhibitory and antioxidant activity. Antioxidant activity was measured using the scavenging activity tests against 2,2-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylendiamine (DMPD), superoxide, and hydrogen peroxide radicals and the metal-related methods; metal-chelating capacity and ferric-reducing antioxidant power (FRAP), and phosphomolibdenum-reducing antioxidant power (PRAP) assays. Total phenol and flavonoid contents were calculated spectrophotometrically. Chemical composition of the seed oil was identified by gas chromatography-mass spectrometry (GC-MS) and rutin was quantified by liquid chromatography-mass spectrometry (LC-MS). The extracts were more active against butyrylcholinesterase and highly able to scavenge DPPH. The ethyl acetate extract of the aerial parts and the ethanol extract of the stems displayed a remarkable effect in the FRAP assay. None of the extracts had capacity to chelate the iron. According to LC-MS analysis, the richest plant part in terms of rutin was found to be the aerial parts. GC-MS analysis revealed that the seed oil was dominated by oleic (33.15%), linoleic (31.93%), and palmitic (13.15%) acids. (c) 2011 Elsevier Ltd. All rights reserved.
THE FATTY ACID AND MINERAL COMPOSITIONS OF DIFFERENT CHICKPEA CULTIVARS CULTIVATED
(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2018) Kaya, Muharrem; Kan, Asuman; Yilmaz, Azime; Karaman, Ruziye; Sener, Aykut
In this study, crude oil content, fatty acid and mineral composition of different chickpea varieties cultivated in Konya were investigated. Fatty acid (saturated and unsaturated) compositions, crude oil and mineral contents were determined; GC-MS, Soxhelet-Henkel and ICP-OES, respectively. There were significant (p<0.01) differences between the chickpea cultivars in view of their crude oil contents and fatty acids (saturated and unsaturated) compositions. The oil yields of the nineteen chickpea cultivars varied between 2.91 and 6.43%. The highest crude oil content was determined in Gokce (6.43%) cultivar. On the other hand, highest compositions of the unsaturated fatty acids were determined in Uzunlu 99 (88.01%). Furthermore, the total compositions of the highest saturated fatty acids were determined in Camtez 87 (17.71%). The linolenic (in ILC 482, 2.68%), linoleic (in ILC 482, 57.25%) and oleic (in Gtiliimser, 42.30%) acids were dominating fatty acids of the cultivars. According to the results, the total macro element composition of the chickpea cultivars were found to range from 16267 to 23634 and K and P were the dominating macro elements of the cultivars. Moreover, the micro element composition of the chickpea cultivars varied from 165.36 to 269.09 Fe and B were the highest micro elements of the cultivars.
In vitro antiviral activities under cytotoxic doses against herpes simples type-1 and parainfluensa-3 viruses of Cicer arietinum L. (Chickpea)
(ACADEMIC JOURNALS, 2009) Kan, Asuman; Ozcelik, Berrin; Kartal, Murat
The objective of this study was to evaluation antiviral activities of the extracts from the seed, fruit skin and aerial parts of ten registered varieties Cicer arietinum (Chickpea) against Herpes simplex type 1 (HSV-1) and Parainfluenza-3 (PI-3) viruses. Madin-Darby Bovine Kidney and Vero cell lines were employed for antiviral assessment of the Cicer arientinum L. extracts, in which acyclovir for HSV-1 and oseltamivir for PI-3 were tested as reference drugs. This is the first report showing that C. arietinum seed extracts of Cse-5 possesses significant antiviral activity both of DNA (32 - 4 mu g ml(-1)) and RNA (32 16 mu g ml(-1)) viruses compared to the fruit skin and aerial part extracts as well as the controls. Besides, the extracts of fruit skin (Cfs-4) and aerial parts (Cap-5) showed remarkable activity against DNA viruses at 32 - 1 mu g ml-(1).
In-vitro Neuroprotective Properties of the Maydis Stigma Extracts From Four Corn Varieties
(Taylor & Francis Ltd, 2012) Kan, Asuman; Orhan, Ilkay; Coksarı, Gulay; Sener, Bilge
Neuroprotective effect of the ethyl acetate and ethanol extracts of the silks of four Zea mays L. varieties (var. intendata, var. indurata, var. everta and var. saccharata) was evaluated by acetylcholinesterase, butrylcholinesterase and tyrosinase inhibitions and by antioxidant activity tests against 2,2-diphenyl-1-picrylhydrazyl and superoxide as well as two iron-related antioxidant methods (iron-chelation capacity and ferric-reducing antioxidant power). Total phenol and flavonoid contents in the extracts were determined spectrophotometrically.
A new plant for Turkey; Buckwheat (Fagopyrum esculentum)
(2014) Kan, Asuman
Türkiye, topografik yapısı ve farklı ekolojik koşulları nedeniyle, dünyanın çok önemli gen merkezi konumundadır. Türkiye aynı zamanda pek çok bitki türünün kültürünün kolaylıkla yapılabileceği farklı agro ekolojik özelliklere sahip dünyanın nadir ülkelerinden birisidir. Türkiye'nin bu ekolojik zenginlikleri nedeni ile yurt dışından temin edilen ve yabancı orijinli bitkilerin kolaylıkla yetiştirilmesinde de ev sahipliği yapmaktadır. Ülkemiz tarım alanlarına son yıllarda yeni giren bitkilerden bir tanesi de karabuğday bitkisidir. Çölyak hastaları tarafından tüketilmesi zorunlu olan gıda ürünlerin başında gelen karabuğday bitkisi yurt dışından introdüksiyon materyali olarak Türkiye'ye girmiştir. Karabuğday bitkisi üzerinde Türkiye'de farklı kurum ve kuruluşlar tarafından hem üretim hem de Ar-Ge çalışmaları yürütülmektedir. Karabuğday (Fagopyrum esculentuntum Moench) kuzukulağıgiller (Polygonaceae) familyasından bir bitki türüdür. Bu çalışma ile, dünyada ve ülkemizde her geçen gün kullanımı artan karabuğdayın bazı bitkisel, kimyasal ve tarımsal özelliklerinin daha çok faydalanıcıya tanıtılması amaçlanmıştır
Orta Anadolu şartlarında ekmeklik buğday ıslahında kullanılabilecek uygun ebeveyn ve melezlerin çoklu dizi (linex tester) yöntemi ile belirlenmesi
(Selçuk Üniversitesi Fen Bilimleri Enstitüsü, 2000-04-21) Kan, Asuman; Sade, Bayram
Orta Anadolu şartlan için uygun ekmeklik buğday melez ve ebeveynlerini belirlemek amacıyla Konya'da üç ekmeklik buğday çeşidi ile 10 ekmeklik buğday hattı arasında 1995 - 1996 üretim yılında çoklu dizi yöntemine göre melezlemeler (30 melez kombinasyonu) yapılmıştır. Bu melezler ebeveynleri ile birlikte 1996 - 1997 üretim yılında yetiştirilmiştir. Fi bitkileri ve ebeveynler üzerinde tek bitki dane verimi, başakta başakçık sayısı, başak uzunluğu, başakta dane sayısı, başakta dane ağırlığı, bitki boyu, üst boğum arası uzunluğu, fertil kardeş sayısı, bin dane ağırlığı, ham protein oranı, kuru öz (gluten) oranı ve sedimantasyon değeri ölçüm, sayım, tartım ve analizleri yapılmıştır. İncelenen özellikler için ebeveyn ve melezlerin çoklu dizi analiz yöntemine göre genel ve özel kombinasyon yetenekleri, heterosis ve heterobeltiosis değerleri, geniş ve dar anlamda kalıtım dereceleri ortaya konmuş ve özellikler arası ilişkiler belirlenmiştir. Tek bitki dane verimi ile danede protein ve gluten oram için eklemeli olmayan gen etkileri ve düşük dar anlamda kalıtım dereceleri bulunmuştur. Tek bitki dane verimi, ham protein ve gluten oranında heterosis değeri pozitif olurken, heterobeltiosis değeri negatif olmuştur. Bu bilgilerin ışığı altında ekmeklik buğdayda verim ve kalite ıslah çalışmalarında kullanılabilecek uygun ebeveyn ve kombinasyonlar belirlenmiştir. 3 ve7 no'lu hatlar hem verim ve hemde kalite, 6 ve 9 no'lu hatların sadece verim potansiyeli, 2 ve 5 nolu hatlar ile Kıraç 66 çeşidi sadece yüksek kalite yönüyle ekmeklik buğday ıslahında kullanılabilecek uygun ebeveynler olarak belirlenmiştir.
A Simple Semi-preparative Reversed-phase HPLC/PDA Method for Separation and Quantification of Glycyrrhizin in Nine Samples of Glycyrrhiza glabra Root Collected from Different Geographical Origins
(WILEY, 2014) Basar, Norazah; Talukdar, Anupam D.; Nahar, Lutfun; Stafford, Angela; Kushiev, Habibjon; Kan, Asuman; Sarker, Satyajit D.
Introduction - Glycyrrhiza glabra L. (Fabaceae), commonly known as 'liquorice', is one of the most popular ingredients in several traditional herbal medicinal preparations, and glycyrrhizin is the major glycoside present in this plant. The content of glycyrrhizin may vary among G. glabra samples collected from various geographical origins, which may affect the therapeutic efficacy. Thus, quantification of glycyrrhizin in G. glabra samples is important. Objective - To develop and validate a simple semi-preparative reversed-phase HPLC with photodiode array (PDA) method for separation and quantification of glycyrrhizin in nine samples of G. glabra root collected from various geographical origins. Methods - Dried and ground root of G. glabra was Soxhlet-extracted sequentially with n-hexane and methanol (MeOH). The separation and quantification of glycyrrhizin was achieved on a C-18 reversed-phase semi-preparative column using a gradient mobile phase, 30-100% solvent B in solvent A in 30 min (solvent A: 0.1% v/v trifluoroacetic acid (TFA) in water and solvent B: 0.1% v/v of TFA in MeOH), at a flow rate of 3.00 mL/min and UV detection at 254 nm. Results - A simple semi-preparative reversed-phase HPLC/PDA method allowing clear separation and quantification of glycyrrhizin content in nine samples has been validated in terms of linearity, selectivity, limits of detection, precision, accuracy and detection. Concentration levels of glycyrrhizin were between 0.177 and 0.688% w/w of dry materials. Conclusion - This method is precise, less time consuming and more cost effective, and can be used for the quality control of any G. glabra sample with regard to its glycyrrhizin contents. Copyright (C) 2014 John Wiley & Sons, Ltd.
A Study on Genotype X Environment Interaction in Chickpea Cultivars (Cicer Arietinum L.) Grown in Arid and Semi-Arid Conditions
(Academic Journals, 2010) Kan, Asuman; Kaya, Muharrem; Gürbüz, Ayşegül; Şanlı, Arif; Özcan, Kamil; Çiftçi, Cemalettin Yaşar
The study was carried out to identify stability and adaptability of 19 chickpea (Cicer arietinum L.) cultivars grown in arid and semi arid conditions at three locations (Field Crops Central Research Institute in Ankara, Bahri Dagdas International Agricultural Research Institute in Konya and Research Farms of Agriculture Faculty of Suleyman Demirel University in Isparta) for two years (2005 and 2006 years). Experiments was set up as randomized complate block design with three replications. Studied parameters were plant height, first pod height, 100 - grain weight and grain yield and stability parameters were calculated according to Finlay-Wilkinson and Ketata methods. Results showed (confirmed) that Menemen 92 (4) and Izmir 92 (6) were the highest yielding and stable cultivars at three locations during the study period in terms of plant height, first pod height and grain yield. For the 100 grain weight, Cagatay (2), Akcin 91 (9) and Er 99 (13) cultivars performed better than other cultivars.

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