Yazar "Mohammed, Bilal Osamah Mohammed" seçeneğine göre listele

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    The determination of some phenotypic and genotypic characteristics of group c streptococcus isolates from different sources
    (Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü, 2019) Mohammed, Bilal Osamah Mohammed; Uçkun Sait Uçan
    The aim of this study was the detection of group C streptococci in 115 isolates from the laboratory collection of Microbiology Department of the Veterinary Faculty, Selcuk University that had been collected from different sources and the determination of some of their phenotypic and genotypic characteristics. This included the evaluation of biofilm production, susceptibility to different antibiotics, detection of SZP virulence factor gene and the determination of sequence variation among strains and genotyping by RAPD method. Streptococcus equi subspecies zooepidemicus ATCC 35246 was used as a standard strain. In this study, 41 of the isolates were confirmed to be group C streptococci by using Latex Streptococcus grouping kit. The identification was completed by both biochemical and molecular methods. The whole strains were Streptococcus equi subspecies zooepidemicus. The biofilm formation was evaluated by three different methods (congo red agar, crystal violet staining method and SEM microscope). In spite of the slight incompatibility among the congo red agar and crystal violet staining methods the majority of isolates were confirmed as strongly biofilm producing strains. The testing of antimicrobial susceptibility revealed a relatively high sensitivity to five antibiotics and mild resistance to the sixth antibiotic. The virulent gene SZP was detected in 35 of the isolates including the reference strain ATCC 35246. The SZP sequence was confirmed to be variable among different strains after comparing all the sequences and generating the phylogenic tree. The genotyping by RAPD method revealed the high variability among strains and also similarity between some of them. In conclusion, group C streptococci originating from the horse respiratory system were found variable.
  • Küçük Resim
    Öğe
    The evaluation of biofilm production by Streptococcus equi zooepidemicus isolates
    (Selçuk Üniversitesi, 2020) Mohammed, Bilal Osamah Mohammed; Uçan, Uçkun Sait
    Amaç: Streptococcus equi zooepidemicus izolatlarının biofilm üretme kapasitelerinin ölçülmesi amaçlandı. Gereç ve Yöntem: Selçuk Üniversitesi Veteriner Fakültesi Mikrobiyoloji Anabilim Dalı kültür koleksiyonundan sağlanan kırk adet Streptococcus equi zooepidemicusizolatı Kongo kırmızısı agar, Kristal moru boyama ve taramalı electron mikroskobu (TEM) olmak üzere 3 farklı yöntem ile biofilm oluşturma yönünden değerlendirildi. Bulgular: Kongo kırmızısı agar ile Kristal moru boyama sonuçları arasında uyumsuzluk vardı. Otuz bir pozitif suşun 19’u Kongo kırmızısı agarda kuvvetli pozitif verirken 30’u Kristal moru boyama ile kuvvetli pozitif bulundu. Biofilm üretimi TEM ile doğrulandı. Öneri: Ülkede at hekimliği hizmeti veren klinisyenlerin, Streptococcus equi zooepidemicus etkenlerinin in vitro şartlarda biofilm oluşturabileceğinin farkında olmaları önerilir.